- Clone
- WM53 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- IV M-505
- Other Names
- Siglec-3, gp67, p67
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
303437 | 100 µg | 190€ | ||||
303438 | 1 mg | 551€ |
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD33 is a 67 kD type I transmembrane glycoprotein also known as Siglec-3, gp67, and p67. It is a sialoadhesion immunoglobulin superfamily member expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. CD33 is absent on normal platelets, lymphocytes, erythrocytes and hematopoietic stem cells. CD33 functions as a sialic acid-dependent cell adhesion molecule with carbohydrate/lectin binding activity.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human myeloid leukaemia cells.
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
CyTOF® - Verified
ICC, IP, WB - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting3, induction of cytokine production3, and immunofluorescence4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 303437 & 303438).
-
Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Favaloro E, et al. 1988. Br. J. Haematol. 69:163.
- Garnache-Ottou F, et al. 2005. Blood 105:1256. (WB)
- Pèrez-Oliva AB, et al. 2011. Glycobiology. 21:757. (epitope, FC, IF)
- RRID
-
AB_2814130 (BioLegend Cat. No. 303437)
AB_2814130 (BioLegend Cat. No. 303438)
Antigen Details
- Structure
- Ig superfamily, sialoadhesins, type I glycoprotein, 67 kD
- Distribution
-
Myeloid progenitor, monocytes, granulocytes, dendritic cells, mast cells
- Function
- Adhesion and lectin activity
- Ligand/Receptor
- Sugar chains containing sialic acid
- Cell Type
- B cells, Dendritic cells, Granulocytes, Hematopoietic stem and progenitors, Mast cells, Monocytes, Neutrophils
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules, Siglec Molecules
- Antigen References
-
1. Favaloro E, et al. 1988. Br. J. Haematol. 69:163.
2. Freeman S, et al. 1995. Blood 85:2005. - Gene ID
- 945 View all products for this Gene ID
- UniProt
- View information about CD33 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD33 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
APC anti-human CD33
Human peripheral blood lymphocytes, monocytes, and granulocy... -
PE anti-human CD33
Human peripheral blood lymphocytes, monocytes and granulocyt... -
PE/Cyanine5 anti-human CD33
-
Purified anti-human CD33
Human peripheral blood lymphocytes, monocytes, and granulocy... -
PerCP/Cyanine5.5 anti-human CD33
Human peripheral blood monocytes were stained with CD33 (clo... -
Brilliant Violet 711™ anti-human CD33
Human peripheral blood monocytes were stained with CD33 (clo... -
Brilliant Violet 421™ anti-human CD33
Human peripheral blood monocytes were stained with CD33 (clo... -
Brilliant Violet 570™ anti-human CD33
Human peripheral blood monocytes were stained with CD33 (clo... -
Purified anti-human CD33 (Maxpar® Ready)
Human PBMCs stained with 166Er-anti-CD44 (BJ18) and 158Gd-an... -
Brilliant Violet 510™ anti-human CD33
Human peripheral blood monocytes were stained with CD33 (clo... -
Biotin anti-human CD33
Human peripheral blood monocytes were stained with biotinyla... -
Brilliant Violet 785™ anti-human CD33
Human peripheral blood monocytes were stained with Brilliant... -
Brilliant Violet 650™ anti-human CD33
Human peripheral blood monocytes were stained with CD33 (clo... -
PE/Cyanine7 anti-human CD33
Human peripheral blood monocytes were stained with a mixture... Human peripheral blood lymphocytes, monocytes, and granulocy... -
PE/Dazzle™ 594 anti-human CD33
Human peripheral blood monocytes were stained with a mixture... Human peripheral blood lymphocytes, monocytes, and granulocy... -
APC anti-human CD33
Typical results from human peripheral blood lymphocytes, mon... -
Alexa Fluor® 700 anti-human CD33
Human peripheral blood monocytes were stained with human CD3... -
Ultra-LEAF™ Purified anti-human CD33
Human peripheral blood lymphocytes, monocytes, and granulocy... -
APC/Fire™ 750 anti-human CD33
Human peripheral blood monocytes were stained with CD33 (clo... -
PerCP/Cyanine5.5 anti-human CD33
Typical results from human peripheral blood monocytes staine... -
APC/Cyanine7 anti-human CD33
Human peripheral blood monocytes were stained with anti-huma... -
PE anti-human CD33
Typical results from human peripheral blood lymphocytes, mon... -
PE/Cyanine7 anti-human CD33
Typical results from human peripheral blood monocytes staine... -
GMP PerCP/Cyanine5.5 anti-human CD33
Typical results from human peripheral blood monocytes staine... -
Alexa Fluor® 647 anti-human CD33
Human peripheral blood monocytes were stained with anti-huma... -
GMP APC anti-human CD33
Typical results from human peripheral blood monocytes staine... -
GMP PE anti-human CD33
Typical results from human peripheral blood monocytes staine... -
PE/Fire™ 810 anti-human CD33
Human peripheral blood monocytes were stained with anti-huma... -
PE/Cyanine5 anti-human CD33
Typical results from human peripheral blood monocytes staine... -
Spark Blue™ 515 anti-human CD33
Human peripheral blood cells were surface stained with (left... -
PE/Fire™ 700 anti-human CD33
Human peripheral blood monocytes were stained with anti-huma... -
GMP PE/Cyanine5 anti-human CD33
Typical results from human peripheral blood monocytes staine... -
Spark Violet™ 423 anti-human CD33
Human peripheral blood monocytes were stained with anti-huma... -
Brilliant Violet 605™ anti-human CD33
Human peripheral blood monocytes were stained with anti-huma...
Follow Us