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Purified anti-human CD48 Antibody

Pricing & Availability
Clone
BJ40 (See other available formats)
Regulatory Status
RUO
Other Names
Blast-1, SLAMF2, HuLy-m3
Isotype
Mouse IgG1, κ
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Product Citations
publications
BJ40_Pure_021009.jpg
Human peripheral blood lymphocytes stained with purified BJ40, followed by anti-mouse IgG FITC
  • BJ40_Pure_021009.jpg
    Human peripheral blood lymphocytes stained with purified BJ40, followed by anti-mouse IgG FITC
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336702 100 µg 85€
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Description

CD48 is a 40-47 kD GPI-anchored membrane protein, also known as Blast-1 and HuLy-m3. It is a member of the CD2 family that contains 2 IgSF domains and is widely expressed on both resting and activated hematopoietic cells with the exception of granulocytes, platelets, and erythrocytes. CD48 binds to CD2 at a considerably (>100-fold) lower affinity than CD58. It is thought to contribute to T cell activation. The cytoplasmic tail of CD48 has been shown to bind to the kinases Lck and Fyn.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, it is recommended to use at ≤ 2.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation.

Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. 1997. Leucocyte Typing VI:White Cell Differentiation Antigens. Garland Publishing Inc.
  2. Wang R, et al. 2012. J. Leukoc Biol. 91:299. PubMed
Product Citations
  1. Sander J et al. 2017. Immunity. 47(6):1051-1066 . PubMed
  2. Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
RRID
AB_1227561 (BioLegend Cat. No. 336702)

Antigen Details

Structure
CD2 family, contains 2 IgSF domains, GPI-membrane anchored, 40-47 kD
Distribution

Widely expressed on hematopoietic cells except granulocytes, platelets, and erythrocytes. Increased on T and B cells after activation

Function
Contributes to T cell antigen recognition
Ligand/Receptor
CD2
Cell Targets
Lck, Fyn interact with cytoplasmic domain
Cell Type
B cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Fisher RC and Thorley-Lawson DA. 1991. Mol. Cell. Biol. 11:1614.
  2. Korinek V, et al. 1991. Immunogenetics 33:108.
  3. Leukocyte Typing IV. Knapp W, et al. (Eds) Oxford University Press (1989)
  4. Leukocyte Typing V. Schlossman S, et al. (Eds) Oxford University Press (1995)
Gene ID
962 View all products for this Gene ID
UniProt
View information about CD48 on UniProt.org
Go To Top Version: 2    Revision Date: 08.18.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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