- Clone
- IT2.2 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- VI CD86.8
- Other Names
- B7-2, B70
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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305435 | 100 µg | 169€ |
CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, monocytes/macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is the ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce costimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can bind to CD152 as well, also known as CTLA-4, to deliver an inhibitory signal to T cells.
Product DetailsProduct Details
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Baboon, Capuchin Monkey, Common Marmoset, Cotton-topped Tamarin, Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
CyTOF® - Verified - Recommended Usage
-
This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6, Western blotting3, and blocking of T cell activation2,4,5. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 305449 & 305450).
- Additional Product Notes
-
Maxpar® is a registered trademark of Standard BioTools Inc.
-
Application References
(PubMed link indicates BioLegend citation) -
- Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
- Dieu M. 1998. J. Exp. Med. 188:373. (Block)
- Esser M, et al. 2001. J. Virol. 75:6173. (WB)
- Jeannin P, et al. 1999. J. Immunol. 162:2044. (Block)
- Kapsogeorgou EK, et al. 2001. J. Immunol. 166:3107. (Block)
- Geissmann F, et al. 2001. Blood 97:1241. (IHC)
- Product Citations
-
- RRID
-
AB_2563764 (BioLegend Cat. No. 305435)
Antigen Details
- Structure
- Ig superfamily, single-chain transmembrane glycoprotein, 80 kD
- Distribution
-
Monocytes/macrophages, activated B cells and T cells, dendritic cells
- Function
- T cell costimulation
- Ligand/Receptor
- CD28, CD152
- Cell Type
- B cells, Dendritic cells, Macrophages, Monocytes, T cells, Tregs
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Hathcock K, et al. 1996. Adv. Immunol. 62:131.
2. June C, et al. 1994. Immunol. Today 15:321. - Gene ID
- 942 View all products for this Gene ID
- UniProt
- View information about CD86 on UniProt.org
Related FAQs
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
-
We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
-
We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
-
We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
-
The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
Other Formats
View All CD86 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-human CD86
Human peripheral blood monocytes were stained with biotinyla... -
PE anti-human CD86
Human peripheral blood monocytes stained with IT2.2 PE -
PE/Cyanine5 anti-human CD86
Human peripheral blood monocytes were stained with CD86 (clo... -
Purified anti-human CD86
Human peripheral blood monocytes were stained with purified ... -
APC anti-human CD86
Human peripheral blood monocytes stained with IT2.2 APC -
Alexa Fluor® 488 anti-human CD86
Human peripheral blood monocytes with IT2.2 Alexa Fluor®... -
Alexa Fluor® 647 anti-human CD86
Human peripheral blood monocytes stained with IT2.2 Alexa Fl... -
Pacific Blue™ anti-human CD86
Human peripheral blood monocytes stained with IT2.2 Pacific ... -
PE/Cyanine7 anti-human CD86
Human peripheral blood monocytes stained with IT2.2 PE/Cyani... -
PerCP/Cyanine5.5 anti-human CD86
Human peripheral blood monocytes were stained with CD86 (clo... -
Brilliant Violet 421™ anti-human CD86
Human peripheral blood monocytes were stained with CD86 (clo... -
Brilliant Violet 650™ anti-human CD86
Human peripheral blood monocytes were stained with CD86 (clo... -
Brilliant Violet 605™ anti-human CD86
Human peripheral blood monocytes were stained with CD86 (clo... -
Brilliant Violet 510™ anti-human CD86
Human peripheral blood monocytes were stained with CD86 (clo... -
PE/Dazzle™ 594 anti-human CD86
Human peripheral blood monocytes were stained with CD86 (clo... -
Purified anti-human CD86 (Maxpar® Ready)
Human PBMCs stained with 156Gd-anti-CD86 [B7.2] (IT2.2). Mon... -
Brilliant Violet 711™ anti-human CD86
Human peripheral blood monocytes were stained with CD86 (clo... -
Brilliant Violet 785™ anti-human CD86
Human peripheral blood monocytes were stained with CD86 (clo... -
TotalSeq™-A0006 anti-human CD86
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TotalSeq™-B0006 anti-human CD86
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TotalSeq™-C0006 anti-human CD86
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Ultra-LEAF™ Purified anti-human CD86
Human peripheral blood monocytes were stained with LEAF™ pur... -
KIRAVIA Blue 520™ anti-human CD86
Human peripheral blood monocytes were stained with anti-huma... -
TotalSeq™-D0006 anti-human CD86
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PE/Fire™ 810 anti-human CD86
Human peripheral monocytes were stained with CD86 (clone IT2... -
PE/Fire™ 640 anti-human CD86
Human peripheral blood cells were blocked with True-Stain Mo... -
PerCP/Fire™ 806 anti-human CD86
Human peripheral blood monocytes were stained with anti-huma... -
PerCP/Fire™ 780 anti-human CD86
Human peripheral blood monocytes were stained with anti-huma... Human peripheral blood monocytes were stained with anti-huma... -
PE/Fire™ 700 anti-human CD86
Human peripheral blood monocytes were stained with anti-huma...
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