- Clone
- IT2.2 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- VI CD86.8
- Other Names
- B7-2, B70
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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305449 | 100 µg | 181€ | ||||
305450 | 1 mg | 524€ |
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, monocytes/macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is the ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce costimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can bind to CD152 as well, also known as CTLA-4, to deliver an inhibitory signal to T cells.
Product DetailsProduct Details
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Baboon, Capuchin Monkey, Common Marmoset, Cotton-topped Tamarin, Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
IHC-F, WB - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6, Western blotting3, and blocking of T cell activation2,4,5. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 305449 & 305450).
-
Application References
(PubMed link indicates BioLegend citation) -
- Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
- Dieu M. 1998. J. Exp. Med. 188:373. (Block)
- Esser M, et al. 2001. J. Virol. 75:6173. (WB)
- Jeannin P, et al. 1999. J. Immunol. 162:2044. (Block)
- Kapsogeorgou EK, et al. 2001. J. Immunol. 166:3107. (Block)
- Geissmann F, et al. 2001. Blood 97:1241. (IHC)
- RRID
-
AB_2814140 (BioLegend Cat. No. 305449)
AB_2814140 (BioLegend Cat. No. 305450)
Antigen Details
- Structure
- Ig superfamily, single-chain transmembrane glycoprotein, 80 kD
- Distribution
-
Monocytes/macrophages, activated B cells and T cells, dendritic cells
- Function
- T cell costimulation
- Ligand/Receptor
- CD28, CD152
- Cell Type
- B cells, Dendritic cells, Macrophages, Monocytes, T cells, Tregs
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Hathcock K, et al. 1996. Adv. Immunol. 62:131.
2. June C, et al. 1994. Immunol. Today 15:321. - Gene ID
- 942 View all products for this Gene ID
- UniProt
- View information about CD86 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD86 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-human CD86
Human peripheral blood monocytes were stained with biotinyla... -
PE anti-human CD86
Human peripheral blood monocytes stained with IT2.2 PE -
PE/Cyanine5 anti-human CD86
Human peripheral blood monocytes were stained with CD86 (clo... -
Purified anti-human CD86
Human peripheral blood monocytes were stained with purified ... -
APC anti-human CD86
Human peripheral blood monocytes stained with IT2.2 APC -
Alexa Fluor® 488 anti-human CD86
Human peripheral blood monocytes with IT2.2 Alexa Fluor®... -
Alexa Fluor® 647 anti-human CD86
Human peripheral blood monocytes stained with IT2.2 Alexa Fl... -
Pacific Blue™ anti-human CD86
Human peripheral blood monocytes stained with IT2.2 Pacific ... -
PE/Cyanine7 anti-human CD86
Human peripheral blood monocytes stained with IT2.2 PE/Cyani... -
PerCP/Cyanine5.5 anti-human CD86
Human peripheral blood monocytes were stained with CD86 (clo... -
Brilliant Violet 421™ anti-human CD86
Human peripheral blood monocytes were stained with CD86 (clo... -
Brilliant Violet 650™ anti-human CD86
Human peripheral blood monocytes were stained with CD86 (clo... -
Brilliant Violet 605™ anti-human CD86
Human peripheral blood monocytes were stained with CD86 (clo... -
Brilliant Violet 510™ anti-human CD86
Human peripheral blood monocytes were stained with CD86 (clo... -
PE/Dazzle™ 594 anti-human CD86
Human peripheral blood monocytes were stained with CD86 (clo... -
Purified anti-human CD86 (Maxpar® Ready)
Human PBMCs stained with 156Gd-anti-CD86 [B7.2] (IT2.2). Mon... -
Brilliant Violet 711™ anti-human CD86
Human peripheral blood monocytes were stained with CD86 (clo... -
Brilliant Violet 785™ anti-human CD86
Human peripheral blood monocytes were stained with CD86 (clo... -
TotalSeq™-A0006 anti-human CD86
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TotalSeq™-B0006 anti-human CD86
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TotalSeq™-C0006 anti-human CD86
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Ultra-LEAF™ Purified anti-human CD86
Human peripheral blood monocytes were stained with LEAF™ pur... -
KIRAVIA Blue 520™ anti-human CD86
Human peripheral blood monocytes were stained with anti-huma... -
TotalSeq™-D0006 anti-human CD86
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PE/Fire™ 810 anti-human CD86
Human peripheral monocytes were stained with CD86 (clone IT2... -
PE/Fire™ 640 anti-human CD86
Human peripheral blood cells were blocked with True-Stain Mo... -
PerCP/Fire™ 806 anti-human CD86
Human peripheral blood monocytes were stained with anti-huma... -
PerCP/Fire™ 780 anti-human CD86
Human peripheral blood monocytes were stained with anti-huma... Human peripheral blood monocytes were stained with anti-huma... -
PE/Fire™ 700 anti-human CD86
Human peripheral blood monocytes were stained with anti-huma...
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