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Ultra-LEAF™ Purified anti-mouse DR3 (TNFRSF25) Antibody

Pricing & Availability
Clone
4C12 (See other available formats)
Regulatory Status
RUO
Other Names
Apo-3, LARD, TNFR25, TRAMP, WSL-1, DR3
Isotype
Armenian Hamster IgG
Ave. Rating
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Product Citations
publications
a-4C12_LEAF_Purified_DR3_TNFRSF25_FC_2_082112
TNFRSF25 transfected p815 cells were stained with LEAF™ purified DR3 (TNFRSF25, clone 4C12) (filled histogram) or LEAF™ purified Armenian hamster IgG (open histogram), followed by anti-hamster-PE.
  • a-4C12_LEAF_Purified_DR3_TNFRSF25_FC_2_082112
    TNFRSF25 transfected p815 cells were stained with LEAF™ purified DR3 (TNFRSF25, clone 4C12) (filled histogram) or LEAF™ purified Armenian hamster IgG (open histogram), followed by anti-hamster-PE.
  • b-4C12_LEAF_Purified_DR3_TNFRSF25_FC_1_082112
    TNFRSF25 transfected p815 cells were stained with LEAF™ purified DR3 (TNFRSF25, clone 4C12) (filled histogram) or LEAF™ purified Armenian hamster IgG (open histogram), followed by anti-hamster-PE.
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Cat # Size Price Quantity Check Availability Save
144411 100 µg 212€
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144412 1 mg 551€
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

DR3, also known as TNFRSF25, lymphocyte-associated receptor of death (LARD), WSL-1, TRAMP, TR3 and APO3, is a 55 kD glycoprotein belonging to the TNF receptor superfamily (TNFRSF). TNFSF25 is expressed at low level on CD4-positive, CD8-positive T cells and NKT cells. It has been shown to stimulate NF-κB activity and regulate cell apoptosis. The signal transduction of this receptor is mediated by various death domains containing adaptor proteins. Recently, it has been shown that TNFRSF25 is also expressed on Treg cells and regulates Treg proliferation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mtnfr25-Ig fusion protein
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Agonist - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

4C12 is an agonistic antibody. It leads to rapid and selective proliferation of pre-existing Tregs in vivo through TCR engagement with MHC class II (MHC-II) and IL-2 signaling. We recommend our Ultra-LEAF™ purified 4C12 antibody (Cat. No. 144411 and 144412) for agonist or in vivo studies.

Application References

(PubMed link indicates BioLegend citation)
  1. Fang L, et al. 2008. J. Exp. Med. 205:1037.
  2. Wolf D, et al. 2012. Transplantation. 94:569. PubMed
  3. Schreiber TH, et al. 2012. J. Immunol. 189:3311.
  4. Longman RS, et al. 2014. J Exp Med. 211:1571. PubMed
Product Citations
  1. Marfil-Garza BA, et al. 2022. Am J Transplant. 22:1101. PubMed
RRID
AB_2800672 (BioLegend Cat. No. 144411)
AB_2800672 (BioLegend Cat. No. 144412)

Antigen Details

Structure
55 kD glycoprotein, contains one death domain and four TNFR-Cys repeats
Distribution

CD4, CD8, NKT and Tregs at low level

Function
Plays a role in lymphocyte homeostasis, induces NFKB activation and apoptosis
Ligand/Receptor
TL1-A
Cell Type
NKT cells, T cells, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction
Molecular Family
Cytokine/Chemokine Receptors
Antigen References
  1. Taraban VY, et al. 2011. J. Clin. Invest. 121:463.
  2. Slebioda TJ, et al. 2011. Eur. J. Immunol. 41:2606.
  3. Schreiber TH, et al. 2010. J. Clin. Invest. 120:3629.
  4. Reddy PB, et al. 2012. J. Virol. 2012. [Epub].
Gene ID
85030 View all products for this Gene ID
UniProt
View information about DR3 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 03.28.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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