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Alexa Fluor® 700 anti-mouse/human CD44 Antibody

Pricing & Availability
Clone
IM7 (See other available formats)
Regulatory Status
RUO
Other Names
Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
1_IM7_Alx700_020608
C57BL/6 mouse splenocytes stained with IM7 Alexa Fluor® 700
  • 1_IM7_Alx700_020608
    C57BL/6 mouse splenocytes stained with IM7 Alexa Fluor® 700
  • 2_53_Mouse_Liver_CD8_CD44_NK1.1
    Confocal image of C57BL/6 mouse liver sample acquired using the IBEX method of highly multiplexed antibody-based imaging: CD8 (cyan) in Cycle 2, CD44 (blue) in Cycle 2, and NK1.1 (red) in Cycle 3. Tissues were prepared using ~1% (vol/vol) formaldehyde and a detergent. Following fixation, samples are immersed in 30% (wt/vol) sucrose for cryoprotection. Images are courtesy of Drs. Andrea J. Radtke and Ronald N. Germain of the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
Compare all formats See Alexa Fluor® 700 spectral data
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103025 25 µg £65
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103026 100 µg £148
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Description

CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Chimpanzee, Baboon, Cynomolgus, Rhesus, Squirrel Monkey, Horse, Cow, Pig, Dog, Cat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Dexamethasone-induced myeloid leukemia M1 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The CD44 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

SB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633nm / 635nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD4417,18 that is located between amino acids 145 and 18620. This clone has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F). Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections6,7, complement-mediated cytotoxicity1, immunoprecipitation1,3, in vivo inhibition of DTH4,5, and spatial biology (IBEX)23,24. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103046, 103065 - 103069).

Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.

Additional Product Notes

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

Application References

(PubMed link indicates BioLegend citation)
  1. Trowbridge IS, et al. 1982. Immunogenetics 15:299. (ICFC, IP, CMCD)
  2. Katoh S, et al. 1994. J. Immunol. 153:3440. (ELISA)
  3. Budd RC, et al. 1987. J. Immunol. 138:3120. (IP)
  4. Camp RL, et al. 1993. J. Exp. Med. 178:497. (Block)
  5. Weiss JM, et al. 1997. J. Cell Biol. 137:1137. (Block)
  6. Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC) PubMed
  7. Cuff CA, et al. 2001. J. Clin. Invest. 108:1031. (IHC)
  8. Lee JW, et al. 2006. Nature Immunol. 8:181.
  9. Zhang N, et al. 2005. J. Immunol. 174:6967. PubMed
  10. Huabiao C, et al. 2005. J. Immunol. 175:591. PubMed
  11. Gui J, et al. 2007. Int. Immunol. 19:1201. PubMed
  12. Wang XY, et al. 2008. Blood 111:2436. PubMed
  13. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  14. Yamazaki J, et al. 2009. Blood PubMed
  15. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  16. Chen YW, et al. 2010. Mol. Cancer Ther. 9:2879. PubMed
  17. Zheng Z, et al. 1995. J. Cell. Biol. 130:485.
  18. Wiranowska M, et al. 2010. Int. J. Cancer 127:532.
  19. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  20. Sandmaier BM, et al. 1998. Blood 91:3494.
  21. Yang Y, et al. 2015. Hypertension. 65:1047. PubMed
  22. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  23. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  24. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Bradley CP et al. 2017. Cell host & microbe. 22(5):697-704 . PubMed
  2. Zheng X, et al. 2019. PLoS Pathog. 15:e1008036. PubMed
  3. Hudson WH, et al. 2020. Immunity. 51(6):1043-1058.e4.. PubMed
  4. Soon MSF, et al. 2020. Nat Immunol. 1.984027778. PubMed
  5. Schenkel JM, et al. 2021. Immunity. 54:2338. PubMed
  6. Grebinoski S, et al. 2022. Nat Immunol. 23:868. PubMed
  7. Campisi L, et al. 2022. Nature. 606:945. PubMed
  8. VanDyke D, et al. 2022. Cell Rep. 41:111478. PubMed
  9. Briukhovetska D, et al. 2023. Immunity. 56:143. PubMed
  10. Ai L, et al. 2023. Cell Discov. 9:9. PubMed
  11. Konjar S, et al. 2022. Proc Natl Acad Sci U S A. 119:e2202144119. PubMed
  12. Wu Z, et al. 2023. EMBO Rep. 24:e56524. PubMed
  13. Le DT, et al. 2023. iScience. 26:106059. PubMed
  14. Dijkman K, et al. 2023. NPJ Vaccines. 8:66. PubMed
  15. Denk D, et al. 2022. Immunity. 55:2059. PubMed
  16. Nguyen NDNT, et al. 2020. NPJ Vaccines. 5:7. PubMed
  17. Chen H, et al. 2005. J Immunol. 175:591. PubMed
  18. Krummey SM, et al. 2020. Cell Reports. 30(5):1282-1291.e5.. PubMed
  19. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  20. Wang D, et al. 2018. Immunity. 48:659. PubMed
  21. Zhang P et al. 2019. Cell Rep. 27(11):3117-3123 . PubMed
  22. Muthumani K, et al. 2015. Sci Transl Med. 7: 301ra132. PubMed
  23. Webb LMC, et al. 2021. Aging Cell. 20:e13295. PubMed
  24. Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed
  25. Uzhachenko RV, et al. 2021. Cell Reports. 35(1):108944. PubMed
  26. Manivasagam S, et al. 2022. J Immunol. 208:1341. PubMed
  27. Rao E, et al. 2021. Sci Immunol. 6:. PubMed
  28. Clemmensen HS, et al. 2021. MBio. 12:. PubMed
  29. Pack AD, et al. 2021. Cell Reports. 36:109586. PubMed
  30. Lu SX, et al. 2021. Cell. . PubMed
  31. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  32. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  33. Konrath KM, et al. 2022. Cell Rep. 38:110318. PubMed
  34. Woodworth JS, et al. 2021. Nat Commun. 12:6658. PubMed
  35. Marangoni F, et al. 2021. Cell. . PubMed
  36. Perner C, et al. 2020. Immunity. 53(5):1063-1077.e7. PubMed
  37. Best SA, et al. 2018. Cell Metab. 27:935. PubMed
  38. Wiesner D, et al. 2015. PLoS Pathog. 11:1004701. PubMed
  39. Panciera T, et al. 2016. Cell Stem Cell. 19:725-737. PubMed
  40. Wei H, et al. 2016. J Immunol. 196: 3537 - 3541. PubMed
  41. Dekhtiarenko I, et al. 2013. J Immunol. 190:3399. PubMed
  42. Hojo MA, et al. 2019. Nat Commun. 2.224305556. PubMed
  43. Ng SS, et al. 2020. Nat Immunol. 21:1205. PubMed
  44. Silver J, et al. 2020. Methods Mol Biol. 2121:115. PubMed
  45. Mirando AC, et al. 2020. Oncoimmunology. 9:1760685. PubMed
  46. Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
  47. Carty S, et al. 2014. PLoS One. 9:106659. PubMed
  48. Clark GC, et al. 2022. Front Oncol. 12:913656. PubMed
  49. Andersen L, et al. 2020. Cell Reports. 29(13):4447-4459.e6.. PubMed
  50. Bosnjak B, et al. 2019. Front Immunol. 10:840. PubMed
  51. Holz LE et al. 2018. Cell reports. 25(1):68-79 . PubMed
  52. Beura LK, et al. 2018. Immunity. 48:327. PubMed
  53. Akkaya B, et al. 2017. J Immunol Methods. 441:67. PubMed
  54. Severance AL, et al. 2022. iScience. 25:104400. PubMed
  55. Rive CM, et al. 2022. Mol Ther Methods Clin Dev. 26:4. PubMed
  56. Ben-Yehuda H, et al. 2021. Mol Neurodegener. 16:39. PubMed
  57. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  58. Campesato LF, et al. 2020. Nat Commun. 3.24375. PubMed
  59. Cabrera-Perez J, et al. 2016. J Immunol. 197: 1692 - 1698. PubMed
  60. Cabrera-Perez C, et al. 2015. J Immunol . 194:1609-20. PubMed
  61. Papa I, et al. 2017. Nature. 547:318. PubMed
  62. Nelson CE et al. 2019. Cell Rep. 28(12):3092-3104 . PubMed
  63. Miyajima M,et al. 2017. Nat Immunol.. 10.1038/ni.3867. PubMed
  64. Emily A Thompson et al. 2019. Cell reports. 26(11):2859-2867 . PubMed
  65. Raju S, et al. 2020. Cell Reports. 29(9):2556-2564.e3.. PubMed
  66. Rouleau N, et al. 2020. Immunohorizons. 0.695833333. PubMed
  67. Elliot TAE, et al. 2021. Immunity. 54:2481. PubMed
  68. Vijay R, et al. 2021. J Exp Med. 218:. PubMed
  69. Filskov J, et al. 2019. Sci Rep. 9:14085. PubMed
  70. Pei B, et al. 2015. J Immunol. 194:5872. PubMed
  71. Vijay R, et al. 2020. Nat Immunol. 21:790. PubMed
  72. Little A, et al. 2016. Oncogenesis. 5:e261. PubMed
  73. Kumar R, et al. 2020. Cell Reports. 30(8):2512-2525. PubMed
  74. Buchlis G, et al. 2013. J Immunol. 191:228. PubMed
  75. Alterauge D, et al. 2020. Cell Rep. 33:108232. PubMed
  76. Gonzalez-Figueroa P, et al. 2021. Cell. 184(7):1775-1789.e19. PubMed
  77. Tsai S, et al. 2018. Cell Metab. 28:922. PubMed
RRID
AB_493712 (BioLegend Cat. No. 103025)
AB_493712 (BioLegend Cat. No. 103026)

Antigen Details

Structure
Variable splicing of CD44 gene generates many CD44 isoforms, 80-95 kD
Distribution

All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells

Function
Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM
Ligand/Receptor
Hyaluronan, MIP-1β, fibronectin, collagen
Cell Type
B cells, Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Haynes BF, et al. 1991. Cancer Cells 3:347.
3. Goldstein LA, et al. 1989. Cell 56:1063.
4. Mikecz K, et al. 1995. Nat. Med. 1:558.
5. Hegde V, et al. 2008. J. Leukocyte Biol. 84:134.
6. Liu T, et al. 2009. Biol. Direct 4:40.

Gene ID
12505 View all products for this Gene ID 960 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD44
Specificity Alt (DOES NOT SHOW ON TDS):
CD44
App Abbreviation (DOES NOT SHOW ON TDS):
FC,SB
UniProt
View information about CD44 on UniProt.org

Related FAQs

If an antibody clone has been previously successfully used in IBEX in one fluorescent format, will other antibody formats work as well?

It’s likely that other fluorophore conjugates to the same antibody clone will also be compatible with IBEX using the same sample fixation procedure. Ultimately a directly conjugated antibody’s utility in fluorescent imaging and IBEX may be specific to the sample and microscope being used in the experiment. Some antibody clone conjugates may perform better than others due to performance differences in non-specific binding, fluorophore brightness, and other biochemical properties unique to that conjugate.

Will antibodies my lab is already using for fluorescent or chromogenic IHC work in IBEX?

Fundamentally, IBEX as a technique that works much in the same way as single antibody panels or single marker IF/IHC. If you’re already successfully using an antibody clone on a sample of interest, it is likely that clone will have utility in IBEX. It is expected some optimization and testing of different antibody fluorophore conjugates will be required to find a suitable format; however, legacy microscopy techniques like chromogenic IHC on fixed or frozen tissue is an excellent place to start looking for useful antibodies.

Are other fluorophores compatible with IBEX?

Over 18 fluorescent formats have been screened for use in IBEX, however, it is likely that other fluorophores are able to be rapidly bleached in IBEX. If a fluorophore format is already suitable for your imaging platform it can be tested for compatibility in IBEX.

The same antibody works in one tissue type but not another. What is happening?

Differences in tissue properties may impact both the ability of an antibody to bind its target specifically and impact the ability of a specific fluorophore conjugate to overcome the background fluorescent signal in a given tissue. Secondary stains, as well as testing multiple fluorescent conjugates of the same clone, may help to troubleshoot challenging targets or tissues. Using a reference control tissue may also give confidence in the specificity of your staining.

How can I be sure the staining I’m seeing in my tissue is real?

In general, best practices for validating an antibody in traditional chromogenic or fluorescent IHC are applicable to IBEX. Please reference the Nature Methods review on antibody based multiplexed imaging for resources on validating antibodies for IBEX.

Other Formats

View All CD44 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse/human CD44 IM7 FC
Biotin anti-mouse/human CD44 IM7 FC,ICC,IHC-P
FITC anti-mouse/human CD44 IM7 FC,ICC,IHC-P
PE/Cyanine5 anti-mouse/human CD44 IM7 FC
Purified anti-mouse/human CD44 IM7 FC,IHC-F,CyTOF®,ELISA,ICC,IHC-P,IP,CMCD,Stim
Brilliant Violet 605™ anti-mouse/human CD44 IM7 FC
PE anti-mouse/human CD44 IM7 FC
Alexa Fluor® 488 anti-mouse/human CD44 IM7 FC,ICC,IHC-P,SB
Alexa Fluor® 647 anti-mouse/human CD44 IM7 FC,ICC,SB
Pacific Blue™ anti-mouse/human CD44 IM7 FC
Alexa Fluor® 700 anti-mouse/human CD44 IM7 FC,SB
PE/Cyanine7 anti-mouse/human CD44 IM7 FC
APC/Cyanine7 anti-mouse/human CD44 IM7 FC
PerCP/Cyanine5.5 anti-mouse/human CD44 IM7 FC
PerCP anti-mouse/human CD44 IM7 FC
Brilliant Violet 421™ anti-mouse/human CD44 IM7 FC,ICC,IHC-P
Brilliant Violet 570™ anti-mouse/human CD44 IM7 FC
Brilliant Violet 785™ anti-mouse/human CD44 IM7 FC
Brilliant Violet 510™ anti-mouse/human CD44 IM7 FC
Ultra-LEAF™ Purified anti-mouse/human CD44 IM7 FC,CyTOF®,ELISA,IHC,IP,CMCD,Stim
Brilliant Violet 650™ anti-mouse/human CD44 IM7 FC
Purified anti-mouse/human CD44 (Maxpar® Ready) IM7 FC,CyTOF®
Alexa Fluor® 594 anti-mouse/human CD44 IM7 ICC,FC,IHC-P,SB
PE/Dazzle™ 594 anti-mouse/human CD44 IM7 FC
Brilliant Violet 711™ anti-mouse/human CD44 IM7 FC
APC/Fire™ 750 anti-mouse/human CD44 IM7 FC
TotalSeq™-A0073 anti-mouse/human CD44 IM7 PG
TotalSeq™-C0073 anti-mouse/human CD44 IM7 PG
TotalSeq™-B0073 anti-mouse/human CD44 IM7 PG
Spark YG™ 570 anti-mouse/human CD44 IM7 FC,IHC-P
Spark YG™ 593 anti-mouse/human CD44 IM7 FC
TotalSeq™-D0073 anti-mouse/human CD44 IM7 PG
Brilliant Violet 750™ anti-mouse/human CD44 IM7 FC
PerCP/Fire™ 806 anti-mouse/human CD44 IM7 FC
Spark Red™ 718 anti-mouse/human CD44 IM7 FC
PE/Fire™ 810 anti-mouse/human CD44 IM7 FC
Spark Blue™ 550 anti-mouse/human CD44 (Flexi-Fluor™) IM7 FC
Spark Red™ 718 anti-mouse/human CD44 (Flexi-Fluor™) IM7 FC
Go To Top Version: 3    Revision Date: 09/11/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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