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PE/Cyanine7 anti-mouse IgD Antibody

Pricing & Availability
Clone
11-26c.2a (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin D
Isotype
Rat IgG2a, κ
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Product Citations
publications
11-26C2A_PECyanine7_IgD_Antibody_1_041024
C57BL/6 splenocytes were stained with CD45R/B220 FITC and IgD (clone 11-26c.2a) PE/Cyanine7 (left) or rat IgG2a, κ PE/Cyanine7 isotype control (right).
  • 11-26C2A_PECyanine7_IgD_Antibody_1_041024
    C57BL/6 splenocytes were stained with CD45R/B220 FITC and IgD (clone 11-26c.2a) PE/Cyanine7 (left) or rat IgG2a, κ PE/Cyanine7 isotype control (right).
Compare all formats See PE/Cyanine7 spectral data
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405719 25 µg £89
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405720 100 µg £233
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Description

Surface IgD is an important B cell differentiation marker.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2,3, and spatial biology (IBEX)10,11.

Application References

(PubMed link indicates BioLegend citation)
  1. Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC)
  2. Weih D, et al. 2001. J. Immunol. 167:1909. (IHC)
  3. Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
  4. Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed
  5. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  6. Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
  7. Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed
  8. Park SY, et al. 2013. J. Immunol. 190:1094. PubMed
  9. Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed
  10. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Lee JH, et al. 2022. Cell Rep. 38:110485. PubMed
  2. Yang K, et al. 2022. Sci Immunol. 7:eabn2888. PubMed
  3. Grégoire C, et al. 2022. Immunity. 55:1216. PubMed
  4. Melzi E, et al. 2022. Immunity. 55:2168. PubMed
  5. Zhang J, et al. 2021. MedComm (Beijing). 2:256. PubMed
  6. Zhu X, et al. 2017. Arch Oral Biol. 10.1016/j.archoralbio.2017.03.010. PubMed
  7. Tu X, et al. 2022. Nat Commun. 13:6977. PubMed
  8. Bourque J, et al. 2021. Heliyon. 7:e08311. PubMed
  9. Alameh MG, et al. 2021. Immunity. 54:2877. PubMed
  10. Wang X, et al. 2021. EMBO J. 40:e105926. PubMed
  11. Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed
  12. Werner A, et al. 2021. iScience. 24:103076. PubMed
  13. Escolano A, et al. 2016. Cell. 166(6):1445-1458.e12. PubMed
  14. Lederer K, et al. 2020. Immunity. 53(6):1281-1295.e5. PubMed
  15. Stienne C, et al. 2022. Cell Rep. 38:110553. PubMed
RRID
AB_2561875 (BioLegend Cat. No. 405719)
AB_2561875 (BioLegend Cat. No. 405720)

Antigen Details

Structure
Ig family
Distribution

B cells

Function
B cell differentiation
Cell Type
B cells
Biology Area
Immunology
Gene ID
380797 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
IgD
Specificity Alt (DOES NOT SHOW ON TDS):
IgD
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about IgD on UniProt.org
Go To Top Version: 1    Revision Date: 02/20/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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