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PerCP/Cyanine5.5 anti-human CD56 (NCAM) Antibody

Pricing & Availability
Clone
HCD56 (See other available formats)
Regulatory Status
RUO
Other Names
Leu-19, NKH1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
HCD56_PerCPCyanine55_CD56_Antibody_1_060719
Human peripheral lymphocytes were stained with CD16 APC and CD56 (NCAM) (clone HCD56) PerCP/Cyanine5.5 (left) or Mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right).
  • HCD56_PerCPCyanine55_CD56_Antibody_1_060719
    Human peripheral lymphocytes were stained with CD16 APC and CD56 (NCAM) (clone HCD56) PerCP/Cyanine5.5 (left) or Mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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318321 25 tests £121
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318322 100 tests £262
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Description

CD56 is a single transmembrane glycoprotein also known as NCAM (Neural Cell Adhesion Molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK cells and NK-T cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparin sulfate.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The CD56 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and 564 nm and a maximun emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone HCD56 is not recommended for immunohistochemistry formalin-fixed paraffin-embedded tissue.

Additional Product Notes
View more applications data for this product in our Scientific Poster Library.
Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  2. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
Product Citations
  1. Vielle NJ, et al. 2018. Sci Rep. 8:5440. PubMed
  2. Shemesh A, et al. 2022. J Exp Med. 219: . PubMed
  3. Edwards CL, et al. 2023. J Clin Invest. 133: . PubMed
  4. Zhang B, et al. 2023. Signal Transduct Target Ther. 8:28. PubMed
  5. Shen Q, et al. 2022. Cancer Res Commun. 2:258. PubMed
  6. Montes de Oca M, et al. 2016. Cell Rep. 17:399-412. PubMed
  7. de Jonge K et al. 2019. Scientific reports. 9(1):4487 . PubMed
  8. Kuang Z, et al. 2020. Antib Ther. 3:227. PubMed
  9. Yokoyama K, et al. 2022. Front Immunol. 13:1016914. PubMed
  10. Walk J, et al. 2019. Nat Commun. 10:874. PubMed
  11. Jürchott K, et al. 2016. PLoS One. 11: 0150812. PubMed
  12. Ponath V, et al. 2021. Int J Mol Sci. 22:. PubMed
  13. Webb K et al. 2018. Frontiers in immunology. 2.574305556 . PubMed
  14. Shemesh A, et al. 2022. J Exp Med. 219:. PubMed
  15. Gannon PO, et al. 2020. Cytotherapy. 22(12):780-791. PubMed
  16. Ciccia F, et al. 2012. Ann Rheum Dis. 71:295. PubMed
  17. McDonnell A, et al. 2017. BMC Cancer . 10.1186/s12885-017-3403-5. PubMed
  18. Theurich S et al. 2017. Cell metabolism. 26(1):171-184 . PubMed
  19. Teirlinck A, et al. 2015. Infect Immun . 83: 3732-3739. PubMed
  20. Carrion B, et al. 2021. Neurol Neuroimmunol Neuroinflamm. 8:. PubMed
RRID
AB_893389 (BioLegend Cat. No. 318321)
AB_893389 (BioLegend Cat. No. 318322)

Antigen Details

Structure
Ig superfamily, single transmembrane or GPI-anchored glycoprotein
Distribution

NK cells, T subset, neural tissue, some LGL and myeloid leukemias

Function
Adhesion
Ligand/Receptor
Heparin sulfate
Cell Type
B cells, Leukemia, Mesenchymal Stem Cells, Neurons, NK cells, T cells
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Lanier L, et al. 1991. J. Immunol. 146:4421.
2. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71.
3. Cremer H, et al. 1994. Nature 367:455.

Gene ID
4684 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD56
Specificity Alt (DOES NOT SHOW ON TDS):
CD56
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD56 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 3    Revision Date: 06/07/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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