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Purified anti-human CD18 Antibody

Pricing & Availability
Clone
CBR LFA-1/2 (See other available formats)
Regulatory Status
RUO
Other Names
Integrin β2 subunit, LFA -1 β subunit, β2 integrin, ITGB2, b2 integrin, beta 2 integrin
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
CBRspaceLFA-1slash2_Purified_CD18_Antibody_FC_010815
Human peripheral blood lymphocytes were stained with purified CD18 (clone CBR LFA1/2) (filled histogram), or purified mouse IgG1, κ isotype control (open histogram), and was then followed by anti-mouse IgG FITC.
  • CBRspaceLFA-1slash2_Purified_CD18_Antibody_FC_010815
    Human peripheral blood lymphocytes were stained with purified CD18 (clone CBR LFA1/2) (filled histogram), or purified mouse IgG1, κ isotype control (open histogram), and was then followed by anti-mouse IgG FITC.
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366302 100 µg £89
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Description

CD18, also known as integrin β2 subunit, LFA-1 β subunit, and β2 integrin, is a 90 - 95 kD type I glycoprotein. CD18 non-covalently associates with CD11a, CD11b, or CD11c. CD18 is expressed on all leukocytes. CD18 and associated α chains function in the adhesion and signaling in hematopoietic cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified human LFA-1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats of this clone) include: activation of LFA-1 and MAC-11,2. The LEAF™ or Ultra-LEAF™ purified antibody (Endotoxin < EU/µg, Azide-Free, 0.2 µm filtered) is recommended for function assays (contact our custom solutions team).

Application References

(PubMed link indicates BioLegend citation)
  1. Petruzzelli L, et al. 1995. J. Immunol. 155:854. (Activ)
  2. Lu C, et al. 2001. J. Immunol. 166:5629. (Activ)
Product Citations
  1. Blandine Monel et al. 2019. Cell reports. 27(1):142-153 . PubMed
RRID
AB_2565276 (BioLegend Cat. No. 366302)

Antigen Details

Structure
90 - 95 kD type I transmembrane glycoprotein.
Distribution

Leukocytes.

Function
Cell adhesion and signaling.
Interaction
Integrin, type I transmembrane glycoprotein, associates with CD11a, CD11b, or CD11c
Ligand/Receptor
ICAMs.
Cell Type
Leukocytes
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Anderson DC, et al. 1987. Annu. Rev. Med. 38:175.
2. Springer TA. 1994. Cell 76:301.

Gene ID
3689 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD18
Specificity Alt (DOES NOT SHOW ON TDS):
CD18
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD18 on UniProt.org
Go To Top Version: 1    Revision Date: 01/21/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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