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Brilliant Violet 421™ anti-mouse CD335 (NKp46) Antibody

Pricing & Availability
Clone
29A1.4 (See other available formats)
Regulatory Status
RUO
Other Names
NKp46, NCR1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
29A1.4_BV421_1_102811
C57BL/6 splenocytes stained with NK1.1 APC and NKp46 (clone 29A1.4) Brilliant Violet 421™ (top) or rat IgG2a Brilliant Violet 421™ isotype control (bottom).
  • 29A1.4_BV421_1_102811
    C57BL/6 splenocytes stained with NK1.1 APC and NKp46 (clone 29A1.4) Brilliant Violet 421™ (top) or rat IgG2a Brilliant Violet 421™ isotype control (bottom).
  • 29A1.4_BV421_2_102811
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
137611 125 µL £140
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137612 50 µg £166
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Description

CD335, also known as NKp46, is a single-pass type I membrane protein of 46 kD. It belongs to the natural cytotoxicity receptor (NCR) family and contains two Ig-like (immunoglobulin-like) domains. It's expression is restricted to NK cells and a subset of NKT cells; it's not expressed in CD1d-restricted NKT cells. CD335 is a receptor for viral hemagglutinins and heparan sulfate proteoglycans and is involved in NK cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
NKP46-IgG1 Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For immunofluorescent staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1,2 and in vitro activation of NK cells1.

Application References

(PubMed link indicates BioLegend citation)
  1. Walzer T, et al. 2007. P. Natl. Acad. Sci. USA 104:3384. (FC, Activ)
  2. Walzer T, et al. 2007. Nat. Immunol. 8:1337. (FC, Activ)
  3. Guerriero JL, et al. 2011. J. Immunol. 186:3517. (IHC) PubMed
Product Citations
  1. Pierson M, et al. 2021. Curr Protoc. 1:e53. PubMed
  2. Dolfi B, et al. 2022. Cell Rep. 39:110949. PubMed
  3. Serra M, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:609. PubMed
  4. Mugarza E, et al. 2022. Sci Adv. 8:eabm8780. PubMed
  5. Luo B, et al. 2022. Oxid Med Cell Longev. 2022:8965903. PubMed
  6. Shen JZ, et al. 2022. Mol Cell. 82:1123. PubMed
  7. Zindl CL, et al. 2022. Immunity. 55:494. PubMed
  8. Ghaffari S, et al. 2022. J Immunol. 208:2246. PubMed
  9. Wu Z, et al. 2023. EMBO Rep. 24:e56524. PubMed
  10. Burrack KS et al. 2018. Immunity. 48(4):760-772 . PubMed
  11. LaMarche NM, et al. 2020. Cell Metabolism. 32(2):243-258.e6. PubMed
  12. Huggins MA, et al. 2019. Cell Rep. 28:1729. PubMed
  13. Maluski M, et al. 2019. J Clin Invest. 129:5108. PubMed
  14. Kelsey E Sivick et al. 2018. Cell reports. 25(11):3074-3085 . PubMed
  15. Alikhanyan K, et al. 2021. Cancers (Basel). 13:. PubMed
  16. Kennedy EM, et al. 2022. Nat Commun. 13:5907. PubMed
  17. Alikhanyan K, et al. 2020. Immun Inflamm Dis. 8:181. PubMed
  18. Shen JZ, et al. 2020. Cell. 184(2):352-369.e23. PubMed
  19. Kaya B, et al. 2020. Cell Reports. 32(5):107979. PubMed
  20. Muthalagu N, et al. 2020. Cancer Discov. 1.022222222. PubMed
  21. Goh W, et al. 2020. Cell Rep. 33:108285. PubMed
  22. Martomo SA, et al. 2021. Mol Cancer Ther. 1.074305556. PubMed
  23. Ponzetta A, et al. 2020. Cell. 178(2):346-360.e24.. PubMed
  24. Kissiov DU, et al. 2022. Elife. 11:. PubMed
  25. Wang X, et al. 2021. Cell. 184:5357. PubMed
  26. Theurich S et al. 2017. Cell metabolism. 26(1):171-184 . PubMed
  27. Wagner AK, et al. 2022. iScience. 25:105137. PubMed
  28. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
RRID
AB_10915472 (BioLegend Cat. No. 137611)
AB_10915472 (BioLegend Cat. No. 137612)

Antigen Details

Structure
Single-pass type I membrane protein, 46 kD; belongs to the natural cytotoxicity receptor (NCR) family; contains 2 Ig-like (immunoglobulin-like) domains
Distribution

Mature and immature NK cells, subset of NKT cells, but not on CD1d-restricted NKT cells

Function
NK cells activation
Ligand/Receptor
Viral hemagglutinins, heparan sulfate proteoglycans
Cell Type
NK cells, NKT cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Colucci F and Cilio CM. 2010. Nat. Immunol. 125:60.
2. Caligiuri MA. 2008. Blood 112:461.
3. Colonna M. 2009. Immunity 31:15.

Gene ID
17086 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD335
Specificity Alt (DOES NOT SHOW ON TDS):
CD335
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD335 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Other Formats

View All CD335 Reagents Request Custom Conjugation
Description Clone Applications
Brilliant Violet 510™ anti-mouse CD335 (NKp46) 29A1.4 FC
Brilliant Violet 711™ anti-mouse CD335 (NKp46) 29A1.4 FC
PE anti-mouse CD335 (NKp46) 29A1.4 FC
PE/Cyanine7 anti-mouse CD335 (NKp46) 29A1.4 FC
Purified anti-mouse CD335 (NKp46) 29A1.4 FC,IHC-F
FITC anti-mouse CD335 (NKp46) 29A1.4 FC
APC anti-mouse CD335 (NKp46) 29A1.4 FC
PerCP/Cyanine5.5 anti-mouse CD335 (NKp46) 29A1.4 FC
Brilliant Violet 421™ anti-mouse CD335 (NKp46) 29A1.4 FC
Biotin anti-mouse CD335 (NKp46) 29A1.4 FC
Brilliant Violet 605™ anti-mouse CD335 (NKp46) 29A1.4 FC
Purified anti-mouse CD335 (NKp46) (Maxpar® Ready) 29A1.4 FC,CyTOF®
Alexa Fluor® 647 anti-mouse CD335 (NKp46) 29A1.4 FC
PE/Dazzle™ 594 anti-mouse CD335 (NKp46) 29A1.4 FC
APC/Fire™ 750 anti-mouse CD335 (NKp46) 29A1.4 FC
Brilliant Violet 650™ anti-mouse CD335 (NKp46) 29A1.4 FC
TotalSeq™-A0184 anti-mouse CD335 (NKp46) 29A1.4 PG
Brilliant Violet 785™ anti-mouse CD335 (NKp46) 29A1.4 FC
Ultra-LEAF™ Purified anti-mouse CD335 (NKp46) 29A1.4 FC,IHC-F
TotalSeq™-B0184 anti-mouse CD335 (NKp46) 29A1.4 PG
TotalSeq™-C0184 anti-mouse CD335 (NKp46) 29A1.4 PG
APC/Cyanine7 anti-mouse CD335 (NKp46) 29A1.4 FC
PE/Cyanine5 anti-mouse CD335 (NKp46) 29A1.4 FC
PerCP/Fire™ 806 anti-mouse CD335 (NKp46) 29A1.4 FC
PerCP/Fire™ 780 anti-mouse CD335 (NKp46) 29A1.4 FC
Spark Red™ 718 anti-mouse CD335 (NKp46) (Flexi-Fluor™) 29A1.4 FC
Spark Blue™ 574 anti-mouse CD335 (NKp46) (Flexi-Fluor™) 29A1.4 FC
Spark Blue™ 550 anti-mouse CD335 (NKp46) (Flexi-Fluor™) 29A1.4 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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