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PE anti-mouse CD335 (NKp46) Antibody

Pricing & Availability
Clone
29A1.4 (See other available formats)
Regulatory Status
RUO
Other Names
NKp46, NCR1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
29A1dot4_PE_030510
C57BL/6 mouse splenocytes stained with 29A1.4 PE and NK1.1 (PK136) APC
  • 29A1dot4_PE_030510
    C57BL/6 mouse splenocytes stained with 29A1.4 PE and NK1.1 (PK136) APC
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137603 25 µg £92
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137604 100 µg £196
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Description

CD335, also known as NKp46, is a single-pass type I membrane protein of 46 kD. It belongs to the natural cytotoxicity receptor (NCR) family and contains two Ig-like (immunoglobulin-like) domains. It's expression is restricted to NK cells and a subset of NKT cells; it's not expressed in CD1d-restricted NKT cells. CD335 is a receptor for viral hemagglutinins and heparan sulfate proteoglycans and is involved in NK cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
NKP46-IgG1 Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1,2 and in vitro activation of NK cells1.

Application References

(PubMed link indicates BioLegend citation)
  1. Walzer T, et al. 2007. P. Natl. Acad. Sci. USA 104:3384. (FC, Activ)
  2. Walzer T, et al. 2007. Nat. Immunol. 8:1337. (FC, Activ)
  3. Guerriero JL, et al. 2011. J. Immunol. 186:3517. (IHC) PubMed
Product Citations
  1. Palacios-Arreola M, et al. 2017. Sci Rep. 10.1038/s41598-017-10135-1. PubMed
  2. Vogt M, et al. 2011. J Virol. 85:11567. PubMed
  3. Hattori K, et al. 2023. Biomed Rep. 18:1. PubMed
  4. Li T, et al. 2023. Nat Commun. 14:2498. PubMed
  5. Fu R, et al. 2020. Sci Rep. 10:1455. PubMed
  6. Fu B et al. 2017. Immunity. 47(6):1100-1113 . PubMed
  7. Li Y, et al. 2014. PLoS One. 9:86927. PubMed
  8. Cluff E, et al. 2022. Cancer Immunol Immunother. . PubMed
  9. Almeida AS, et al. 2021. Bio Protoc. 11:e4012. PubMed
  10. Victorino F, et al. 2015. J Immunol. 195: 4973 - 4985. PubMed
  11. Starkl P, et al. 2020. Immunity. 53(4):793-804.e9. PubMed
  12. Dolgova EV, et al. 2022. Int J Mol Sci. 23:. PubMed
  13. Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed
  14. Best SA, et al. 2018. Cell Metab. 27:935. PubMed
  15. Schaffert A, et al. 2020. Front Immunol. 10:3024. PubMed
  16. Li J, et al. 2020. Cancer Discov. . PubMed
  17. Almeida AS, et al. 2021. Bio Protoc. 11:e4012. PubMed
  18. Li J, et al. 2020. Cancer Immunol Res. 0.529166667. PubMed
  19. Adams NM et al. 2018. Immunity. 48(6):1172-1182 . PubMed
  20. Diaz–Salazar C, et al. 2020. Cell Rep. 32:108186. PubMed
  21. Wiedemann GM, et al. 2020. Cell Rep. 33:108498. PubMed
  22. Progatzky F, et al. 2021. Nature. 599:125. PubMed
  23. Ye X, et al. 2021. Cancer Biol Med. Online ahead of print. PubMed
  24. Ruiz–Manzano RA, et al. 2020. Frontiers in Oncology. 0.927777778. PubMed
  25. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  26. Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
  27. Zhao L,et al. 2017. Nat Commun. . 10.1038/s41467-017-01181-4. PubMed
  28. Sheppard S, et al. 2021. Cell Reports. 35(9):109210. PubMed
  29. Teng F, et al. 2021. Cell Rep. 37:110051. PubMed
  30. Geary CD et al. 2018. Cell reports. 24(8):1949-1957 . PubMed
  31. Liu CF, et al. 2017. Front Immunol. 1.445833333. PubMed
  32. Guo S, et al. 2021. Frontiers in Immunology. 11:614667. PubMed
RRID
AB_10552741 (BioLegend Cat. No. 137603)
AB_10552741 (BioLegend Cat. No. 137604)

Antigen Details

Structure
Single-pass type I membrane protein, 46 kD; belongs to the natural cytotoxicity receptor (NCR) family; contains 2 Ig-like (immunoglobulin-like) domains
Distribution

Mature and immature NK cells, subset of NKT cells, but not on CD1d-restricted NKT cells

Function
NK cells activation
Ligand/Receptor
Viral hemagglutinins, heparan sulfate proteoglycans
Cell Type
NK cells, NKT cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Colucci F and Cilio CM. 2010. Nat. Immunol. 125:60.
2. Caligiuri MA. 2008. Blood 112:461.
3. Colonna M. 2009. Immunity 31:15.

Gene ID
17086 View all products for this Gene ID
UniProt
View information about CD335 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All CD335 Reagents Request Custom Conjugation
Description Clone Applications
Brilliant Violet 510™ anti-mouse CD335 (NKp46) 29A1.4 FC
Brilliant Violet 711™ anti-mouse CD335 (NKp46) 29A1.4 FC
PE anti-mouse CD335 (NKp46) 29A1.4 FC
PE/Cyanine7 anti-mouse CD335 (NKp46) 29A1.4 FC
Purified anti-mouse CD335 (NKp46) 29A1.4 FC,IHC-F
FITC anti-mouse CD335 (NKp46) 29A1.4 FC
APC anti-mouse CD335 (NKp46) 29A1.4 FC
PerCP/Cyanine5.5 anti-mouse CD335 (NKp46) 29A1.4 FC
Brilliant Violet 421™ anti-mouse CD335 (NKp46) 29A1.4 FC
Biotin anti-mouse CD335 (NKp46) 29A1.4 FC
Brilliant Violet 605™ anti-mouse CD335 (NKp46) 29A1.4 FC
Purified anti-mouse CD335 (NKp46) (Maxpar® Ready) 29A1.4 FC,CyTOF®
Alexa Fluor® 647 anti-mouse CD335 (NKp46) 29A1.4 FC
PE/Dazzle™ 594 anti-mouse CD335 (NKp46) 29A1.4 FC
APC/Fire™ 750 anti-mouse CD335 (NKp46) 29A1.4 FC
Brilliant Violet 650™ anti-mouse CD335 (NKp46) 29A1.4 FC
TotalSeq™-A0184 anti-mouse CD335 (NKp46) 29A1.4 PG
Brilliant Violet 785™ anti-mouse CD335 (NKp46) 29A1.4 FC
Ultra-LEAF™ Purified anti-mouse CD335 (NKp46) 29A1.4 FC,IHC-F
TotalSeq™-B0184 anti-mouse CD335 (NKp46) 29A1.4 PG
TotalSeq™-C0184 anti-mouse CD335 (NKp46) 29A1.4 PG
APC/Cyanine7 anti-mouse CD335 (NKp46) 29A1.4 FC
PE/Cyanine5 anti-mouse CD335 (NKp46) 29A1.4 FC
PerCP/Fire™ 806 anti-mouse CD335 (NKp46) 29A1.4 FC
PerCP/Fire™ 780 anti-mouse CD335 (NKp46) 29A1.4 FC
Spark Red™ 718 anti-mouse CD335 (NKp46) (Flexi-Fluor™) 29A1.4 FC
Spark Blue™ 574 anti-mouse CD335 (NKp46) (Flexi-Fluor™) 29A1.4 FC
Spark Blue™ 550 anti-mouse CD335 (NKp46) (Flexi-Fluor™) 29A1.4 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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