Loading...

PE/Cyanine7 anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
Ave. Rating
Submit a Review
Product Citations
publications
HK1dot4_PECy7_083109
C57BL/6 bone marrow cells stained with HK1.4 PE/Cyanine7
  • HK1dot4_PECy7_083109
    C57BL/6 bone marrow cells stained with HK1.4 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
128017 25 µg £85
Check Availability


Need larger quantities of this item?
Request Bulk Quote
128018 100 µg £213
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Application References

(PubMed link indicates BioLegend citation)
  1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
  3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Product Citations
  1. Drobek A, et al. 2015. J Immunol. 195: 3416 - 3426. PubMed
  2. Moyat M, et al. 2015. Infect Immun . 83: 4217 - 4228. PubMed
  3. Dudek M, et al. 2015. Mucosal Immunol. . PubMed
  4. Daemen S, et al. 2021. Cell Reports. 34(2):108626. PubMed
  5. Oliver AJ, et al. 2020. Oncoimmunology. 9:1802979. PubMed
  6. Rosina M, et al. 2022. Cell Metab. 34:533. PubMed
  7. Bernasconi R, et al. 2021. EMBO Mol Med. :e14392. PubMed
  8. Tan YY, et al. 2022. Bio Protoc. 12:e4307. PubMed
  9. Yang Y, et al. 2022. FEBS J. 289:5279. PubMed
  10. Agarwalla P, et al. 2022. Nat Biotechnol. 40:1250. PubMed
  11. Aghayev T, et al. 2022. Cancer Discov. 12:1960. PubMed
  12. Pavliuchenko N, et al. 2023. Front Immunol. 13:1035226. PubMed
  13. Wang L, et al. 2023. Oxid Med Cell Longev. 2023:6480848. PubMed
  14. Boroumand P, et al. 2022. Elife. 11:. PubMed
  15. Li J, et al. 2022. Elife. 11:. PubMed
  16. Wang JX, et al. 2023. JCI Insight. 8:. PubMed
  17. Petenkova A, et al. 2023. Nat Commun. 14:2761. PubMed
  18. Wang C, et al. 2023. Int J Oral Sci. 15:19. PubMed
  19. Garcia-Fabiani MB, et al. 2020. Methods Enzymol. 632:369. PubMed
  20. Stein K, et al. 2016. J Leukoc Biol. 99: 231 - 239. PubMed
  21. Gonçalves NP, et al. 2017. J Neuroinflammation. 14:115. PubMed
  22. Blecher-Gonen R, et al. 2019. Cell Syst. 8:109. PubMed
  23. Albarrán–Juárez J, et al. 2018. J Exp Med. 215:2655. PubMed
  24. Zasłona Z, et al. 2014. J Immunol. 193:4245. PubMed
  25. Clark K, et al. 2015. J Autoimmun. Available online 21 August 2015. PubMed
  26. Choi YY, et al. 2021. Cell Death Dis. 12:826. PubMed
  27. Paubelle E, et al. 2020. Cell Reports. 30(3):739-754.e4.. PubMed
  28. Stolp B, et al. 2022. Cell Rep. 38:110387. PubMed
  29. Brandt S, et al. 2020. J Am Soc Nephrol. 31:2589. PubMed
  30. Kleinholz CL, et al. 2021. Sci Rep. 11:15071. PubMed
  31. LeBlond ND, et al. 2020. J Lipid Res. 61:1697. PubMed
  32. Mathur S, et al. 2021. JCI Insight. 6:. PubMed
  33. Yang F, et al. 2020. Cancer Res. 80:3677. PubMed
  34. Tran L, et al. 2017. Cancer Immunol Res. 5:1141. PubMed
  35. Diao N, et al. 2016. Sci Rep. 6:34672. PubMed
  36. Wang C, et al. 2021. Cell Rep. 37:110021. PubMed
  37. Sugita J, et al. 2021. Nat Commun. 12:1910. PubMed
  38. Fujita Y et al. 2018. Cell reports. 24(12):3296-3311 . PubMed
  39. Syed A, et al. 2015. Infect Immun . 83: 3428-3437. PubMed
  40. Koren N, et al. 2021. Cell Host Microbe. 29(2):197-209.e5. PubMed
  41. Ratitong B, et al. 2021. Cell Reports. 35(7):109139. PubMed
  42. Wang F, et al. 2021. Cell Mol Gastroenterol Hepatol. 13:257. PubMed
  43. Palathingal Bava E, et al. 2022. JCI Insight. 7:. PubMed
  44. Wang Y, et al. 2021. Sci Rep. 1.429861111. PubMed
  45. Alshetaiwi H, et al. 2020. Sci Immunol. 5:00. PubMed
  46. Shami A, et al. 2015. Sci Rep. 5: 13904. PubMed
  47. Wu W, et al. 2018. J Am Soc Nephrol. 29:168. PubMed
  48. Pascual–García M, et al. 2019. Nat Commun. 10:2416. PubMed
  49. Molina M, et al. 2015. PLoS One. 10:127262. PubMed
  50. Tang CC, et al. 2021. eLife. 10:00. PubMed
  51. Zhang F, et al. 2020. EMBO Mol Med. e12034:12. PubMed
  52. Iizasa E, et al. 2021. Nat Commun. 12:2299. PubMed
  53. Geng S, et al. 2021. JCI Insight. 6:. PubMed
  54. Warheit-Niemi HI, et al. 2022. JCI Insight. 7:. PubMed
  55. Du Y, et al. 2022. Nat Commun. 13:231. PubMed
  56. Formaglio P, et al. 2021. Immunity. 54:2724. PubMed
  57. Knizkova D, et al. 2022. Nat Immunol. 23:1644. PubMed
  58. Dmitrieva‐Posocco O et al. 2019. Immunity. 50(1):166-180 . PubMed
  59. Niknam S, et al. 2018. Clin Cancer Res. 24:5735. PubMed
  60. Calcinotto A, et al. 2018. Nat Commun. 9:4832. PubMed
  61. Garg M, et al. 2021. Cell Reports. 34(6):108736. PubMed
  62. Momin N, et al. 2022. Nat Commun. 13:109. PubMed
  63. Pradhan K, et al. 2021. Front Immunol. 12:778830. PubMed
  64. Zhou G, et al. 2019. FASEB J. 33:11396. PubMed
  65. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
  66. Albarrán-Juárez J, et al. 2016. Atherosclerosis. 251:445-53. PubMed
  67. Saha S, et al. 2016. Nat Commun. 7:13096. PubMed
  68. Runyan CE, et al. 2020. Aging Cell. 19:00. PubMed
  69. Gurusamy M, et al. 2021. Nat Commun. 12:6798. PubMed
  70. Steiger S, et al. 2015. J Immunol. 195: 5495 - 5502. PubMed
  71. Yang YS, et al. 2020. Mol Ther Methods Clin Dev. 17:922. PubMed
  72. Hasegawa T, et al. 2020. Sci Rep. 10:13480. PubMed
  73. Perry JL, et al. 2020. ACS Nano. 5.583333333. PubMed
  74. Souza SP, et al. 2021. PLoS Pathog. 17:e1010081. PubMed
  75. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  76. Muri J, et al. 2020. eLife. 9:e53627.. PubMed
  77. Gladow N, et al. 2020. PLoS One. 15:e0227734. PubMed
  78. Nowak W, et al. 2020. EBioMedicine. 50:290-305.. PubMed
  79. Hirata SI, et al. 2020. Allergy. 75:1939. PubMed
  80. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  81. Qadir AS, et al. 2021. iScience. 24:103348. PubMed
  82. Penke LR, et al. 2020. Life Sci Alliance. 3:. PubMed
  83. Weinstock NI, et al. 2020. Neuron. 107(1):65-81. PubMed
  84. Wang X, et al. 2016. J Immunol. 197(12):4750-4761. PubMed
  85. Cartwright ANR, et al. 2021. Cancer Immunol Res. 9:470. PubMed
  86. LeBlond ND, et al. 2020. J Lipid Res. 61:1697. PubMed
  87. Feng L, et al. 2022. Signal Transduct Target Ther. 7:21. PubMed
  88. Rasid O, et al. 2020. Cell Reports. 29(12):3933-3945.e3.. PubMed
  89. Yuan C, et al. 2015. Biochem Biophys Res Commun. 464: 249-255. PubMed
  90. van Geffen C, et al. 2021. Front Immunol. 12:695933. PubMed
  91. Johnson RM, et al. 2021. Front Immunol. 11:614697. PubMed
  92. Porrello A, et al. 2018. Nat Commun. 9:1988. PubMed
  93. Golan K et al. 2018. Cell stem cell. 23(4):572-585 . PubMed
  94. Zhang T, et al. 2020. JCI Insight. 5:00. PubMed
  95. Dai X, et al. 2021. Molecular Cell. 81(11):2317-2331.e6. PubMed
  96. Pradhan K, et al. 2021. J Immunol. 206:2980. PubMed
  97. Liu SS, et al. 2021. Immunity. :. PubMed
  98. Nakayama Y, et al. 2020. Proc Natl Acad Sci U S A. 117:14365. PubMed
  99. E O’Koren, R Mathew, D Saban 2016. Sci Rep. 6:20636. PubMed
  100. Rodriguez-Tirado C, et al. 2022. Cancers (Basel). 14:. PubMed
  101. Zhang J, et al. 2020. Cell Metabolism. 31(6):1136-1153. PubMed
  102. Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed
  103. Clement M, et al. 2014. Proc Natl Acad Sci U S A. 111:1101. PubMed
  104. Ang QY, et al. 2020. Cell. 181(6):1263-1275.e16. PubMed
  105. Dourcy M, et al. 2020. Mucosal Immunol. 13:799. PubMed
  106. Lau A, et al. 2022. Sci Adv. 8:eabm0142. PubMed
  107. Kubli SP, et al. 2019. Nat Commun. 10:2678. PubMed
  108. Syed I et al. 2018. Cell metabolism. 27(2):419-427 . PubMed
  109. Geng S, et al. 2016. Nat Commun. 7:13436. PubMed
  110. Fearon AE, et al. 2021. iScience. 24:103143. PubMed
RRID
AB_1732082 (BioLegend Cat. No. 128017)
AB_1732082 (BioLegend Cat. No. 128018)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
Ly-6C
Specificity Alt (DOES NOT SHOW ON TDS):
Ly-6C
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about Ly-6C on UniProt.org
Go To Top Version: 2    Revision Date: 12/09/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account