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- Clone
- JK1B-1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interleukin-1β, Catabolin, Hematopoietin-1 (H1), IFNβ-inducing factor, Interleukin-β, Osteoclast activating factor (OAF)
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 508201 | 50 µg | £73 | ||||
| 508202 | 500 µg | £238 | ||||
IL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1β is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells. The JK1B-1 antibody reacts with human interleukin-1β (IL-1β) and baboon IL-1β. The JK1B-1 antibody does not recognize mouse IL-1β or human IL-1α or other cytokines. The JK1B-1 antibody can neutralize the bioactivity of natural or recombinant IL-1β.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Baboon
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human IL-1 beta
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ELISA Capture - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2-6 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-1β recombinant protein ranging from 250 to 2 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
ELISA and ELISPOT Capture: The purified JK1B-1 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated JK1B-2 antibody (Cat. No. 508301) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture.
Flow Cytometry: The fluorochrome-labeled JK1B-1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-1β-producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section. -
Application References
(PubMed link indicates BioLegend citation) -
- Lu Y, et al. 2015. PNAS. 112:607. PubMed
- Product Citations
-
- RRID
-
AB_315509 (BioLegend Cat. No. 508201)
AB_315509 (BioLegend Cat. No. 508202)
Antigen Details
- Structure
- Cytokine; 17.3 kD (Mammalian)
- Bioactivity
- Stimulates T cells, B cells, proliferation/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia
- Cell Sources
- Monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma, keratinocytes,
- Cell Targets
- B cells, T cells, monocytes
- Receptors
- Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Bomford R, et al. Eds. 1989. Interleukin-1 inflammation and disease. Elsevier New York.
3. Brazel D, et al. 1991. Biotechnol. Ther. 2:241.
4. Dinarello C. 1996. Blood 87:2095. - Regulation
- Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α2-macroglobulin
- Gene ID
- 3553 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- IL-1beta
- Specificity Alt (DOES NOT SHOW ON TDS):
- IL-1β
- App Abbreviation (DOES NOT SHOW ON TDS):
- ELISA Capture
- UniProt
- View information about IL-1beta on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Does this clone recognize the pro or mature form of IL-1 beta?
-
The monoclonal anti-human IL-1b antibody clone H1b-98 was generated and screened using the mature form of IL-1b as the immunogen. Since the pro-form contains the mature sequence, it is believed that the antibody also recognize pro-IL-1beta. Similarly, we believe clone JK1B-1 should also recognize both pro and mature forms of IL-1b. However, this information has not been confirmed with in-house testing data.
Other Formats
View All IL-1β Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human IL-1β | JK1B-1 | ELISA Capture |
| FITC anti-human IL-1β | JK1B-1 | ICFC |
| Alexa Fluor® 647 anti-human IL-1β | JK1B-1 | ICFC |
| TotalSeq™-B1153 anti-human IL-1β | JK1B-1 | ICPG |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-human IL-1β
-
FITC anti-human IL-1β
LP-stimulated (6 hours) human peripheral blood monocytes su... -
Alexa Fluor® 647 anti-human IL-1β
LPS-stimulated (6 hour) human peripheral blood monocytes sur... -
TotalSeq™-B1153 anti-human IL-1β
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