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Alexa Fluor® 488 anti-Bax Antibody

Pricing & Availability
Clone
2D2 (See other available formats)
Regulatory Status
RUO
Other Names
BCL2 associated X protein, apoptosis regulator Bax
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
2D2_A488_BAX_Antibody_101918
Human B lymphoblast cell line RAMOS was fixed and permeabilized using the True-Nuclear™ Transcription Factor Buffer Set, then stained with Bax (clone 2D2) Alexa Fluor® 488 (filled histogram) or mouse IgG1, κ Alexa Fluor® 488 isotype control (open histogram).
  • 2D2_A488_BAX_Antibody_101918
    Human B lymphoblast cell line RAMOS was fixed and permeabilized using the True-Nuclear™ Transcription Factor Buffer Set, then stained with Bax (clone 2D2) Alexa Fluor® 488 (filled histogram) or mouse IgG1, κ Alexa Fluor® 488 isotype control (open histogram).
See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Check Availability Save
633603 25 µg £109
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633604 100 µg £241
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Description

Bax is a 21 kD pro-apoptotic protein known to regulate apoptosis. Bax is found in the cytoplasm, mitochondria, and nucleus and is highly expressed in hematopoietic stem cells, ovaries, and lymph nodes. Bax binds the anti-apoptotic protein Bcl-2 as a heterodimer or forms homodimers. The relative levels of pro-apoptotic proteins such as Bax and anti-apoptotic proteins such as Bcl-2 determines whether cell death will occur following an apoptotic stimulus. Bax accelerates the opening of mitochondrial VDAC altering membrane potential and allowing cytochrome c to pass out of the mitochondria into the cytosol to initiate downstream caspase activation. p53 can transcriptionally activate the Bax gene to induce apoptosis. Bax has been shown to be mutated in some human cancers. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Amino acids 3-16 of human Bax protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone 2D2 has been shown to be useful for Western blotting1, immunoprecipitation2, immunofluorescence3 and immunohistochemical staining3 of formalin-fixed and paraffin-embedded tissue sections. This antibody does not cross-react with Bcl-2 or Bcl-XL proteins.

Clone 2D2 does not show specificity for mouse BAX.

Application References

(PubMed link indicates BioLegend citation)
  1. Hsu YT, et al. 1997. J. Biol. Chem. 272:13829. (WB)
  2. Cartron PF, et al. 2004. FEBS Lett. 578:41. (IP)
  3. Pucci S, et al. 2009. Cell Cycle 8:473. (IF, IHC)
Product Citations
  1. Yang HL, et al. 2020. Am J Reprod Immunol. 84:e13261. PubMed
  2. Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed
  3. Lin J, Sorger M 2015. Sci Rep. 6: 8390. PubMed
  4. Herrera FG, et al. 2019. Int J Radiat Oncol Biol Phys. 103:320. PubMed
RRID
AB_2562172 (BioLegend Cat. No. 633603)
AB_2562172 (BioLegend Cat. No. 633604)

Antigen Details

Structure
Forms homodimers and heterodimers with Bcl-2, approximately 21 kD
Distribution

Cytoplasm, mitochondria, nucleus; expressed in hematopoietic stem cells, ovaries, lymph nodes

Function
Induces cell death through the process of apoptosis by acting on mitochondria
Interaction
14-3-3 theta, VDAC1, Bcl-2, solute carrier family 25, mitochondrial carrier member 4
Cell Type
Hematopoietic stem and progenitors
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Antigen References

1. LeBlanc H, et al. 2002. Nat. Med. 8:274.
2. Marzo I, et al. 1998. Science 281:2027.
3. Miyashita T et al. 1995. Cell 80:293.
4. Oltvai ZN, et al. 1993. Cell 74:609.

Gene ID
581 View all products for this Gene ID
UniProt
View information about Bax on UniProt.org
Go To Top Version: 3    Revision Date: 10/19/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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