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Purified anti-Bax Antibody

Pricing & Availability
Clone
2D2 (See other available formats)
Regulatory Status
RUO
Other Names
BCL2 associated X protein, apoptosis regulator Bax
Isotype
Mouse IgG1, κ
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Product Citations
publications
2D2_Purified_Bax_Antibody_1_031620
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1 µg/mL of purified anti-BAX antibody (clone 2D2) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • 2D2_Purified_Bax_Antibody_1_031620
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1 µg/mL of purified anti-BAX antibody (clone 2D2) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
Cat # Size Price Quantity Check Availability Save
633601 25 µg £70
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633602 100 µg £150
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Description

Bax is a 21 kD pro-apoptotic protein known to regulate apoptosis. Bax is found in the cytoplasm, mitochondria, and nucleus and is highly expressed in hematopoietic stem cells, ovaries, and lymph nodes. Bax binds the anti-apoptotic protein Bcl-2 as a heterodimer or forms homodimers. The relative levels of pro-apoptotic proteins such as Bax and anti-apoptotic proteins such as Bcl-2 determines whether cell death will occur following an apoptotic stimulus. Bax accelerates the opening of mitochondrial VDAC altering membrane potential and allowing cytochrome c to pass out of the mitochondria into the cytosol to initiate downstream caspase activation. p53 can transcriptionally activate the Bax gene to induce apoptosis. Bax has been shown to be mutated in some human cancers. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Amino acids 3-16 of human Bax protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted at between 2°C and 8°C.
Application

WB - Quality tested
IP, IHC-P, ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel.  It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 2D2 has been shown to be useful for Western blotting1, immunoprecipitation2, immunofluorescence3 and immunohistochemical staining3 of formalin-fixed and paraffin-embedded tissue sections. This antibody does not cross-react with Bcl-2 or Bcl-XL proteins.

Clone 2D2 does not show specificity for mouse BAX.

Application References

(PubMed link indicates BioLegend citation)
  1. Hsu YT, et al. 1997. J. Biol. Chem. 272:13829. (WB)
  2. Cartron PF, et al. 2004. FEBS Lett. 578:41. (IP)
  3. Pucci S, et al. 2009. Cell Cycle 8:473. (IF, IHC)
Product Citations
  1. Wang HH, et al. 2022. J Biomed Sci. 29:36. PubMed
  2. Xu ZH, et al. 2021. Mediators Inflamm. 2021:8856326. PubMed
  3. Dang L, et al. 2017. Immunol Lett. 10.1016/j.imlet.2017.06.012. PubMed
  4. Bi R, et al. 2020. Oncol Rep. 44:1939. PubMed
  5. Saranaruk P, et al. 2020. Int J Mol Med. 45:1005. PubMed
  6. Wang F, et al. 2020. Int J Oncol. 57:707. PubMed
  7. Linetsky M, et al. 2020. Free Radic Biol Med. 152:280. PubMed
  8. Karimabad M, et al. 2017. Life Sciences. 10.1016/j.lfs.2017.05.026. PubMed
  9. Lu IT, et al. 2022. Mar Drugs. 20:. PubMed
RRID
AB_2061544 (BioLegend Cat. No. 633601)
AB_2061544 (BioLegend Cat. No. 633602)

Antigen Details

Structure
Forms homodimers and heterodimers with Bcl-2, approximately 21 kD
Distribution

Cytoplasm, mitochondria, nucleus; expressed in hematopoietic stem cells, ovaries, lymph nodes

Function
Induces cell death through the process of apoptosis by acting on mitochondria
Interaction
14-3-3 theta, VDAC1, Bcl-2, solute carrier family 25, mitochondrial carrier member 4
Cell Type
Hematopoietic stem and progenitors
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Antigen References

1. LeBlanc H, et al. 2002. Nat. Med. 8:274.
2. Marzo I, et al. 1998. Science 281:2027.
3. Miyashita T et al. 1995. Cell 80:293.
4. Oltvai ZN, et al. 1993. Cell 74:609.

Gene ID
581 View all products for this Gene ID
UniProt
View information about Bax on UniProt.org
Go To Top Version: 2    Revision Date: 03/16/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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