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Alexa Fluor® 488 anti-human TRA-1-60-R Antibody

Pricing & Availability
Clone
TRA-1-60-R (See other available formats)
Regulatory Status
RUO
Other Names
Podocalyxin, TRA-1
Isotype
Mouse IgM, κ
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Product Citations
publications
Tra-1-60-R_A488_121508.jpg
NCCIT (human teratocarcinoma cell line) stained with TRA-1-60-R Alexa Fluor® 488 (red line) or mouse IgM, κ Alexa Fluor® 488 isotype control(green line).
  • Tra-1-60-R_A488_121508.jpg
    NCCIT (human teratocarcinoma cell line) stained with TRA-1-60-R Alexa Fluor® 488 (red line) or mouse IgM, κ Alexa Fluor® 488 isotype control(green line).
Compare all formats See Alexa Fluor® 488 spectral data
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330613 25 tests £113
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330614 100 tests £221
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Description

TRA-1-60-R antibody recognizes the neuraminidase resistant form of TRA-1-60. TRA-1-60 is a 200-240 kD cell surface antigen expressed by human embryonic stem cells (ESC), embryonic germ cells (EG), and embryonal carcinoma cells. The expression of TRA-1-60 on human ESC is downregulated upon differentiation. The soluble form of TRA-1-60 has been found in the serum of patients with embryonic cancers. Historically, TRA-1-60 is a neuroaminidase-sensitive (TRA-1-60-S) carbohydrate epitope expressed on podocalyxin, a member of the mucin-like glycoprotein CD34 family. Podocalyxin is a type I transmembrane sialoprotein with anti-adhesion properties. Further studies have shown that some of TRA-1-60 is neuraminidase resistant (TRA-1-60-R).  The two forms of TRA-1-60 are essentially indistinguishable in terms of their cellular reactivity except for the difference in neuraminidase sensitivity.  Both of TRA-1-60-S and TRA-1-60-R appear to be the same molecular weight glycoproteins detected by Western blot.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human embryonal carinoma cell line 2102Ep cl.2A6
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy, immunohistochemical staining, acetone-fixed frozen sections, immunoprecipitation, and radioimmunoassay.

Application References

(PubMed link indicates BioLegend citation)
  1. Miyoshi N, et. al. 2010. P. Natl. Acad. Sci. USA 107:40. (IF)
  2. Kuai XL, et al. 2009. Differentiation 77:229. (WB)
  3. King F, et al. 2009. Stem Cells Dev. 18:1441. (FC)
Product Citations
  1. Leavens KF, et al. 2020. Stem Cell Res. 50:102112. PubMed
  2. Krumm L, et al. 2021. Stem Cell Research. 56:102520. PubMed
  3. Slamecka J, et al. 2017. Cell Cycle. 17:330. PubMed
  4. Ababneh NA, et al. 2020. Stem Cell Research. 49:102000. PubMed
  5. Schinzel R, et al. 2011. PLoS One. 6:e27495. PubMed
  6. Handel A, et al. 2016. Hum Mol Genet. 25: 989 - 1000. PubMed
  7. Dong T, et al. 2021. Stem Cell Research. 51:102196. PubMed
  8. Vazquez-Arango P, et al. 2016. Nucleic Acids Res. 44(22):10960-10973. PubMed
  9. Wattanapanitch M, et al. 2014. PLoS One. 9:106952. PubMed
  10. Gagne AL et al. 2018. Stem cell research. 31:157-160 . PubMed
  11. Kuramochi Y, et al. 2021. Stem Cell Research. 53:102337. PubMed
  12. Kaindl J, et al. 2018. Stem Cells Transl Med. 8(3):247-259. PubMed
  13. Dafinca R, et al. 2020. Stem Cell Reports. 14:892. PubMed
  14. Okubo T, et al. 2020. J Biol Chem. 295:3456. PubMed
  15. Ababneh NA, et al. 2020. Stem Cell Research. 48:101967. PubMed
  16. Tsukamoto S, et al. 2021. Stem Cell Research. 53:102363. PubMed
  17. Sommer A et al. 2018. Cell stem cell. 23(1):123-131 . PubMed
  18. Maguire JA et al. 2019. Stem Cell Res. 1723:21:00 . PubMed
  19. Arai Y, et al. 2020. Stem Cell Research. 72.57986111. PubMed
RRID
AB_2119064 (BioLegend Cat. No. 330613)
AB_2119064 (BioLegend Cat. No. 330614)

Antigen Details

Distribution

Embryonic stemcells

Cell Type
Embryonic Stem Cells
Biology Area
Immunology, Stem Cells
Gene ID
5420 View all products for this Gene ID
UniProt
View information about TRA-1-60-R on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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