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Alexa Fluor® 594 anti-mouse CD107a (LAMP-1) Antibody

Pricing & Availability
Clone
1D4B (See other available formats)
Regulatory Status
RUO
Other Names
Lysosome-associated membrane glycoprotein 1, lysosomal membrane glycoprotein 1, CD107 antigen-like family member A, lysosome-associated membrane protein 1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
1D4B_CD107a_Purified_Antibody_IHCF_053019
Thioglycolate-elicited BALB/c mouse peritoneal macrophages were fixed with 1% PFA for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes. Then the cells were stained with 2.5 µg/ml of CD107a (clone 1D4B) Alexa Fluor® 594 (red) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with 40X objective.
  • 1D4B_CD107a_Purified_Antibody_IHCF_053019
    Thioglycolate-elicited BALB/c mouse peritoneal macrophages were fixed with 1% PFA for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes. Then the cells were stained with 2.5 µg/ml of CD107a (clone 1D4B) Alexa Fluor® 594 (red) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with 40X objective.
Compare all formats See Alexa Fluor® 594 spectral data
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121622 100 µg £174
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Description

CD107a, also known as Lysosome-Associated Membrane Protein 1 (LAMP-1) or LGP-120, is a 110-140 kD type I membrane glycoprotein. Mature CD107a is heavily glycosylated from a 40 kD core protein. This molecule is located on the luminal side of lysosomes. Upon activation, CD107a is transferred to the cell membrane surface of activated platelets, activated lymphocytes, macrophages, epithelial cells, endothelial cells, and some tumor cells. CD107a has been suggested to play a role in the protection of lysosomal membrane from lysosomal hydrolases which is involved in cell adhesion and regulation of tumor metastasis, and mediates autoimmune disease progression. CD107a is a ligand for galaptin and E-selectin. Surface expression of LAMP-1 has been shown to correlate with CD8+ T cell and NK cell cytotoxicity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
This monoclonal antibody was raised against NIH/3T3 mouse embryonic fibroblast tissue culture cell membranes. It has been mapped to the N-terminus of LAMP-1.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-F - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 2.5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Product Citations
  1. Griffith AL, et al. 2023. Cell Genom. 100387:3. PubMed
RRID
AB_2563965 (BioLegend Cat. No. 121622)

Antigen Details

Structure
LAMP-1 is a 406 amino acid protein with a molecular mass of 44 kD.
Gene ID
16783 View all products for this Gene ID
UniProt
View information about CD107a on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 09/24/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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