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Alexa Fluor® 594 anti-Pan-Cytokeratin Antibody

Pricing & Availability
Clone
AE-1/AE-3 (See other available formats)
Regulatory Status
RUO
Other Names
Pan-Cytokeratin (AE-1/AE-3), AE1/AE3
Isotype
Mouse IgG1, κ
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Product Citations
publications
a. AE1-AE3_PURE_Cytokeratin_Antibody_081822
Formalin-fixed paraffin-embedded human tonsil tissue stained with purified Alexa Fluor® 594 anti-Pan-Cytokeratin (clone AE-1/AE-3). Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), tissue was incubated with (panel B) or without (panel A) 5.0 µg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI (blue) and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale: 50 µm
  • a. AE1-AE3_PURE_Cytokeratin_Antibody_081822
    Formalin-fixed paraffin-embedded human tonsil tissue stained with purified Alexa Fluor® 594 anti-Pan-Cytokeratin (clone AE-1/AE-3). Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), tissue was incubated with (panel B) or without (panel A) 5.0 µg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI (blue) and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale: 50 µm
  • b. AE1-AE3_PURE_Cytokeratin_Antibody_2_081822
    MCF7 cells were fixed with 4% paraformaldehyde, permeabilized with 0.5% Triton X-100, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with Alexa Fluor® 594 mouse IgG1, κ isotype control (Cat No. 400174) (panel A) or Alexa Fluor® 594 anti-Pan-Cytokeratin (clone AE-1/AE-3) (panel B) overnight at 4°C. Nuclei were counterstained with DAPI and the image was captured with a 60X objective. Scale: 50 µm
  • c.AE1AE3_AF594_Cytokeratin_Antibody_083022
    MCF7 cells (positive control) (closed histogram), and Jurkat cells (negative control) (open histogram) were fixed and permeabilized using Cyto-Fast Fix/Perm Buffer (Cat. No. 426803) and intracellularly stained with anti-Pan-Cytokeratin (clone AE-1/AE-3) Alexa Fluor® 594, or mouse IgG1, κ Alexa Fluor® 594 isotype control (open histogram, dashed line is representative for both Jurkat and MCF7 cells) (Cat No. 400174).
Compare all formats See Alexa Fluor® 594 spectral data
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914205 25 µg £103
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914206 100 µg £192
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Description

AE-1 immunoreacts with an antigenic determinant present on most of the subfamily A cytokeratins, including cytokeratins with molecular weights of 56.5, 50, 48 and 40 kD. AE-3 reacts with an antigenic determinant shared by the subfamily B cytokeratins including cytokeratins with molecular weights of of 64, 59, 58, 56, and 52 kD.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Rat
Reported Reactivity
Dog, Non-Human Primate
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was developed using human epidermal keratins.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
ICC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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RRID
AB_2924628 (BioLegend Cat. No. 914205)
AB_2924628 (BioLegend Cat. No. 914206)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
NA
UniProt
View information about Pan-Cytokeratin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/19/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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