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Purified anti-Pan-Cytokeratin Antibody

Pricing & Availability
Clone
AE-1/AE-3 (See other available formats)
Regulatory Status
RUO
Other Names
Pan-Cytokeratin (AE-1/AE-3), AE1/AE3
Isotype
Mouse IgG1, κ
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Product Citations
publications
AE-1slashAE-3_PURE_Cytokeratin_Antibody_IHC-P_1_103118
IHC staining of purified anti-Pan-Cytokeratin antibody (clone AE-1/AE-3) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 1: Universal, 100X (Cat. No. 927901), the tissue was incubated with 1 µg/ml of the primary antibody for 20 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, AEC, Cat. No. 929401) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • AE-1slashAE-3_PURE_Cytokeratin_Antibody_IHC-P_1_103118
    IHC staining of purified anti-Pan-Cytokeratin antibody (clone AE-1/AE-3) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 1: Universal, 100X (Cat. No. 927901), the tissue was incubated with 1 µg/ml of the primary antibody for 20 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, AEC, Cat. No. 929401) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • AE-1slashAE-3_PURE_Cytokeratin_Antibody_IHC-P_2_103118
    Formalin-fixed paraffin-embedded human tonsil treated with a citrate buffer pH6 and heat for antigen retrieval was stained with purified Pan-Cytokeratin clone AE-1/AE-3, conjugated and detected with a Cy3 conjugated CODEX™ oligonucleotide duplex (red). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
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914204 100 µg £157
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Description

AE-1 immunoreacts with an antigenic determinant present on most of the subfamily A cytokeratins, including cytokeratins with molecular weights of 56.5, 50, 48 and 40 kD. AE-3 reacts with an antigenic determinant shared by the subfamily B cytokeratins including cytokeratins with molecular weights of of 64, 59, 58, 56, and 52 kD.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Rat
Reported Reactivity
Dog, Non-Human Primate
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was developed using human epidermal keratins.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Tiago M, et al. 2020. Br J Cancer. 122:789. PubMed
  2. Metcalf KJ, et al. 2022. Matrix Biol Plus. 14:100105. PubMed
  3. Jiang S, et al. 2022. Immunity. 55:1118. PubMed
  4. Kou R, et al. 2018. Oncol Rep. 7:2013. PubMed
  5. Xie S, et al. 2020. Ann Transl Med. 1.384027778. PubMed
  6. Chang TH, et al. 2020. Cell Death Dis. 11:338. PubMed
  7. Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed
  8. Cai S, et al. 2022. iScience. 25:104980. PubMed
RRID
AB_2616960 (BioLegend Cat. No. 914204)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
NA
UniProt
View information about Pan-Cytokeratin on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 08/16/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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