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Alexa Fluor® 647 anti-Cytochrome c Antibody

Pricing & Availability
Clone
6H2.B4 (See other available formats)
Regulatory Status
RUO
Other Names
Cyt c
Isotype
Mouse IgG1, κ
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Product Citations
publications
1_6H2.B4_AF647_Cytochrome_C_Antibody_ICFC_122414
C57BL/6 splenocytes were treated with BioLegend's Fixation Buffer and Permeabilization Wash Buffer, and then were stained with Cytochrome C (clone 6H2-B4) Alexa Fluor® 647 (filled histogram) or mouse IgG1, κ Alexa Fluor® 647 isotype control (open histogram).
  • 1_6H2.B4_AF647_Cytochrome_C_Antibody_ICFC_122414
    C57BL/6 splenocytes were treated with BioLegend's Fixation Buffer and Permeabilization Wash Buffer, and then were stained with Cytochrome C (clone 6H2-B4) Alexa Fluor® 647 (filled histogram) or mouse IgG1, κ Alexa Fluor® 647 isotype control (open histogram).
  • BH2dotB4_A647_Cytochromec_Antibody_2_101520
    A-431 cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with a 1:250 dilution (2.0 µg/mL) of Alexa Fluor® 647 anti-Cytochrome c Antibody, clone 6H2.B4. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective.
Compare all formats See Alexa Fluor® 647 spectral data
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612310 100 µg £182
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Description

Cytochrome c is a 15 kD protein found in the mitochondrial intermembrane space with a heme-binding domain. Cytochrome c is a component of the electron transport chain; the heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Cytochrome c initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9. Cytochrome c interacts with the cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, and Caspase 9 proteins. The 6H2.B4 monoclonal antibody recognizes human, mouse, and rat cytochrome-c and has been shown to be useful for intracellular flow cytometric staining, Western blotting, immunoprecipitation, and immunofluorescence staining.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat, Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Rat cyt c-OVA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For immunocytochemistry, a concentration range of 2.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: intracellular flow cytometry5, immunofluorescence microscopy3,5, immunoprecipitation4, and immunocytochemistry5.

Application References

(PubMed link indicates BioLegend citation)
  1. Goshorn SC, et al. 1991. J. Biol. Chem. 266:2134.
  2. Jemmerson R, et al. 1991. Eur. J. Immunol. 21:143.
  3. Chandra D, et al. 2002. J. Biol. Chem. 277:50842. (IF)
  4. Semenkova L, et al. 2003. Eur. J. Biochem. 270:4388. (IP)
  5. Shih S-F, et al. 2001. J. Biol. Chem. 276:21870. (ICFC ICC IF)
  6. She P, et al. 2011. Am J. Physiol Endcorinol Metab.301:E49. PubMed
  7. McGuire, KA., et al. 2011. J. Virol 85:10806. PubMed
Product Citations
  1. Hemming ML, et al. 2022. Clin Cancer Res. 28:2397. PubMed
  2. Xu Y, et al. 2023. EMBO Rep. 24:e55503. PubMed
  3. Manzano-Muñoz A, et al. 2022. NPJ Precis Oncol. 6:90. PubMed
  4. Pan R, et al. 2022. Cell. 185:1521. PubMed
  5. Pogacar Z, et al. 2022. Life Sci Alliance. 5:. PubMed
  6. Carter BZ, et al. 2023. Blood Cancer J. 13:57. PubMed
  7. Sekar G, et al. 2022. iScience. 25:105064. PubMed
  8. Alcon C, et al. 2020. Cell Death Dis. 0.898611111. PubMed
  9. Deng J, et al. 2021. Cell Death Dis. 12:978. PubMed
  10. Ramsey HE, et al. 2018. Cancer Discov. 8:1566. PubMed
  11. Meyer LK, et al. 2019. J Clin Invest. 130:863. PubMed
  12. Olesinski EA, et al. 2021. STAR Protocols. 2(2):100461. PubMed
  13. Fraser C, et al. 2022. Nat Commun. 13:5789. PubMed
  14. Bhatt S, et al. 2020. Cancer Cell. 38(6):872-890.e6. PubMed
  15. Jenson J, et al. 2017. Elife. 10.7554/eLife.25541. PubMed
  16. Canedo EC, et al. 2021. JCI Insight. 6:. PubMed
  17. Potter DS, et al. 2021. Cell Death Dis. 12:741. PubMed
  18. Manzano-Muñoz A, et al. 2021. Front Cell Dev Biol. 9:695225. PubMed
  19. Alcon C, et al. 2021. Cells. 10:. PubMed
  20. Victorino F, et al. 2021. eLife. 0.416666666666667. PubMed
  21. Dhimolea E, et al. 2021. Cancer Cell. 39:240. PubMed
  22. Liang Z, et al. 2021. J Immunol. 207:2039. PubMed
RRID
AB_2565241 (BioLegend Cat. No. 612310)

Antigen Details

Structure
Heme binding domain; 15 kD
Distribution

Mitochondrial intermembrane space

Function
Component of electron transport chain; heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9
Interaction
Cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, Casp9
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Mitochondrial Function, Neuroscience, Neuroscience Cell Markers
Molecular Family
Mitochondrial Markers
Antigen References

1. Liu X, et al. 1996. Cell. 86:147.
2. Li P, et al. 1997. Cell. 91:479.
3. Zhang Z, et al. 2003. Gene 312:61.
4. Ferguson H, et al. 2003. J. Biol. Chem. 278:45793.

Gene ID
1355 View all products for this Gene ID
UniProt
View information about Cytochrome c on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 01/02/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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