- Clone
- 9F10 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- V S215
- Other Names
- VLA-4 α chain, α4 integrin, Integrin α4 chain, ITGA4
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
304335 | 25 tests | £105 | ||||
304336 | 100 tests | £217 |
CD49d is a 150 kD α integrin chain known as α4 integrin or VLA-4 α chain. It forms a heterodimer with either integrin β1 (α4β1, VLA-4) or β7 (α4β7). CD49d is expressed broadly on T lymphocytes, B lymphocytes, monocytes, thymocytes, eosinophils, basophils, mast cells, NK cells, dendritic cells, and some non-hematopoietic cells, but not on normal red blood cells, platelets or neutrophils. VLA-4 binds to VCAM-1 (CD106) and fibronectin. α4β7 is the receptor for VCAM-1 and MAdCAM-1. CD49d participates in mononuclear cell trafficking to endothelial sites of inflammation and has roles in cell-cell interactions and cell adhesion to extracellular matrices. CD49d is involved in lymphocyte migration, T cell activation, and hematopoietic stem cell differentiation. CD49d is a marker to isolate pure populations of Treg cells due to its absence on Foxp3+ cells.
Product DetailsProduct Details
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Baboon, Cat, Cow, Chimpanzee, Common Marmoset, Dog, Horse, Sheep, Squirrel Monkey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, and in vitro T cell costimulation2,3. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 304339 and 304340).
-
Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Jeong SH, et al. 2004. J. Virol. 78:6995. (Costim)
- Vogel TU, et al. 2002. J. Immunol. 169:4511. (Costim)
- Kleinewietfeld M, et al. 2009. Blood 113:827. (FC) PubMed
- Palacious F, et al. 2010. Blood 115:4488. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.
- Mattapallil MJ, et al. 2011. J. Immunol. 187:1977. PubMed
- RRID
-
AB_2728240 (BioLegend Cat. No. 304335)
AB_2728241 (BioLegend Cat. No. 304336)
Antigen Details
- Structure
- Integrin, type I transmembrane glycoprotein, 150 kD.
- Distribution
-
T cells, B cells, NK , dendritic cells, thymocytes, monocytes, eosinophils, mast cells.
- Function
- Lymphocyte migration, T cell activation, stem cell differentiation.
- Ligand/Receptor
- Fibronectin, VCAM-1, MAdCAM-1
- Cell Type
- B cells, Dendritic cells, Eosinophils, Mast cells, Monocytes, NK cells, T cells, Thymocytes, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Elices M, Ed.1995. Springer Semin. Immunopathol. 16(4).
2. Lobb RR and Helmer ME. et al. 1994. J. Clin. Invest. 94:1722. - Gene ID
- 3676 View all products for this Gene ID
- UniProt
- View information about CD49d on UniProt.org
Related FAQs
Other Formats
View All CD49d Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
APC anti-human CD49d
Human peripheral blood lymphocytes stained with 9F10 APC -
PE anti-human CD49d
Human peripheral blood lymphocytes stained with 9F10 PE -
PE/Cyanine5 anti-human CD49d
Human peripheral blood lymphocytes stained with 9F10 PE/Cyan... -
Purified anti-human CD49d
Human peripheral blood lymphocytes stained with CD49d (clone... -
Alexa Fluor® 594 anti-human CD49d
Human paraffin-embedded placenta tissue slice was stained wi... -
PerCP/Cyanine5.5 anti-human CD49d
Human peripheral blood lymphocytes stained with 9F10 PerCP/C... -
PE/Cyanine7 anti-human CD49d
Human peripheral blood lymphocytes stained with 9F10 PE/Cyan... -
FITC anti-human CD49d
Human peripheral blood lymphocytes were stained with CD49d (... -
Brilliant Violet 510™ anti-human CD49d
Human peripheral blood lymphocytes were stained with CD49d (... -
Brilliant Violet 421™ anti-human CD49d
Human peripheral blood lymphocytes were stained with CD49d (... -
Purified anti-human CD49d (Maxpar® Ready)
Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 141Pr-an... -
Brilliant Violet 605™ anti-human CD49d
Human peripheral blood lymphocytes were stained with CD49d (... -
PE/Dazzle™ 594 anti-human CD49d
Human peripheral blood lymphocytes were stained with CD49d (... -
APC/Cyanine7 anti-human CD49d
Human peripheral blood lymphocytes were stained with CD49d (... -
Brilliant Violet 711™ anti-human CD49d
Human peripheral blood lymphocytes were stained with CD49d (... -
Alexa Fluor® 647 anti-human CD49d
Human peripheral blood lymphocytes were stained with CD49d (... -
Biotin anti-human CD49d
Human peripheral blood lymphocytes were stained with biotiny... -
TotalSeq™-A0576 anti-human CD49d
-
Brilliant Violet 785™ anti-human CD49d
Human peripheral blood lymphocytes were stained with CD49d (... -
APC/Fire™ 750 anti-human CD49d
Human peripheral blood lymphocytes were stained with CD49d (... -
Ultra-LEAF™ Purified anti-human CD49d
Human peripheral blood lymphocytes stained with CD49d (clone... -
TotalSeq™-C0576 anti-human CD49d
-
TotalSeq™-B0576 anti-human CD49d Antibody
-
TotalSeq™-D0576 anti-human CD49d
-
APC/Fire™ 750 anti-human CD49d
Typical results from human peripheral blood lymphocytes stai...
Follow Us