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Alexa Fluor® 647 anti-human CD66a/c/e Antibody

Pricing & Availability
Clone
ASL-32 (See other available formats)
Regulatory Status
RUO
Other Names
CEACAM1/5/6, BGP, NCA
Isotype
Mouse IgG2b, κ
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Product Citations
publications
1_ASL-32_A647_CD66sce_Antibody_updated_012918
Human colorectal adenocarcinoma cell line HT-29 was stained with CD66a/c/e (clone ASL-32) Alexa Fluor® 647 (filled histogram) or mouse IgG2b, κ Alexa Fluor® 647 isotype control (open histogram).
  • 1_ASL-32_A647_CD66sce_Antibody_updated_012918
    Human colorectal adenocarcinoma cell line HT-29 was stained with CD66a/c/e (clone ASL-32) Alexa Fluor® 647 (filled histogram) or mouse IgG2b, κ Alexa Fluor® 647 isotype control (open histogram).
  • 2_ASL-32_A647_CD66a-c-e_Antibody_2_IHC-P_031318
    Human paraffin-embedded spleen tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate-Buffered 1X pH6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/ml of Alexa Fluor® 647 anti-human CD66a/c/e (clone ASL-32) Antibody (red) and Alexa Fluor® 594 anti-human CD45RO (clone UCHL1) antibody (green) overnight at 4°C. The image was scanned with a 10X objective and stitched with MetaMorph® software.
Compare all formats See Alexa Fluor® 647 spectral data
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342317 25 tests £89
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342318 100 tests £182
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Description

The ASL-32 mAb reacts with an antigen epitope shared by CD66a, c and e. CD66a/c/e are members of the CEA (carcinoembryonic antigen) family of the Ig superfamily. CD66 plays a role in hemophilic and heterophilic adhesion. CD66a, also known as CEACAM1 and BGP (biliary glycoprotein), is mainly expressed on granulocytes, binds to CD66a, c, e, and CD62E. CD66c (known as CEACAM6, NCA) is expressed on granulocytes and epithelial cells. The ligands of CD66c are CD66a-e, CD62E and Galectins. CD66e molecule is also called CEA or CEACAM5, and is primarily found on epithelial cells. CD66e binds to CD66a, c and e.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Shey MS, et al. 2012. J Immunol Methods. 376:79. PubMed.
Product Citations
  1. Xiao Q, et al. 2022. Sci Immunol. 7:eabl3995. PubMed
RRID
AB_2728308 (BioLegend Cat. No. 342317)
AB_2728308 (BioLegend Cat. No. 342318)

Antigen Details

Structure
Member of the CEA (carcinoembryonic antigen) family of the Ig superfamily
Distribution

Granulocytes, epithelial cells

Function
Homophilic and heterophilic adhesion
Ligand/Receptor
CD66a-e, CD62E, galectins
Cell Type
Epithelial cells, Granulocytes
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. Wiley-Liss. A John Wiley & Sons Inc. Publication

Gene ID
634 View all products for this Gene ID
UniProt
View information about CD66a CD66c CD66e on UniProt.org
Go To Top Version: 1    Revision Date: 01/30/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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