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Brilliant Violet 421™ anti-human CD66a/c/e Antibody

Pricing & Availability
Clone
ASL-32 (See other available formats)
Regulatory Status
RUO
Other Names
CEACAM1/5/6, BGP, NCA
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
ASL-32_BV421_CD66ace_Antibody_082317
Human colorectal adenocarcinoma cell line HT-29 was stained with CD66a/c/e (clone ASL-32) Brilliant Violet 421™ (filled histogram) or mouse IgG2b, κ Brilliant Violet 421™ isotype control (open histogram).
  • ASL-32_BV421_CD66ace_Antibody_082317
    Human colorectal adenocarcinoma cell line HT-29 was stained with CD66a/c/e (clone ASL-32) Brilliant Violet 421™ (filled histogram) or mouse IgG2b, κ Brilliant Violet 421™ isotype control (open histogram).
  • ASL-32_BV421_CD66ace_IHC-P_010318
    Human paraffin-embedded spleen tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Citrate-Buffered Saline 1X (pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for 5 minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/ml of Alexa Fluor® 594 anti-CD45RO (clone UCHL1) antibody (red) and Brilliant Violet 421™ anti-CD66a/c/e antibody (blue) overnight at 4°C. The image was scanned with a 10X objective and stitched with MetaMorph® software.
Compare all formats See Brilliant Violet 421™ spectral data
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342313 25 tests £133
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342314 100 tests £275
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Description

The ASL-32 mAb reacts with an antigen epitope shared by CD66a, c and e. CD66a/c/e are members of the CEA (carcinoembryonic antigen) family of the Ig superfamily. CD66 plays a role in hemophilic and heterophilic adhesion. CD66a, also known as CEACAM1 and BGP (biliary glycoprotein), is mainly expressed on granulocytes, binds to CD66a, c, e, and CD62E. CD66c (known as CEACAM6, NCA) is expressed on granulocytes and epithelial cells. The ligands of CD66c are CD66a-e, CD62E and Galectins. CD66e molecule is also called CEA or CEACAM5, and is primarily found on epithelial cells. CD66e binds to CD66a, c and e.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Shey MS, et al. 2012. J Immunol Methods. 376:79. PubMed.
RRID
AB_2716132 (BioLegend Cat. No. 342313)
AB_2716132 (BioLegend Cat. No. 342314)

Antigen Details

Structure
Member of the CEA (carcinoembryonic antigen) family of the Ig superfamily
Distribution

Granulocytes, epithelial cells

Function
Homophilic and heterophilic adhesion
Ligand/Receptor
CD66a-e, CD62E, galectins
Cell Type
Epithelial cells, Granulocytes
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. Wiley-Liss. A John Wiley & Sons Inc. Publication

Gene ID
634 View all products for this Gene ID
UniProt
View information about CD66a CD66c CD66e on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 0    Revision Date: 08/23/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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