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Alexa Fluor® 647 anti-IRF7 Antibody

Pricing & Availability
Clone
12G9A36 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon regulatory factor-7 (IRF-7)
Isotype
Mouse IgG2b, κ
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Product Citations
publications
12G9A36_AF647_IRF7_Antibody_FC_051414.jpg
Overnight starved Jurkat cells were treated with or without IFN-α for 24 hours, then fixed and permeabilized with FOXP3 Fix/Perm Buffer Set. Cells were then stained with IRF7 (clone 12G9A36) Alexa Fluor® 647 (solid line with open histogram for untreated cells, filled histogram for stimulated cells) or mouse IgG2b, κ Alexa Fluor® 647 isotype control (dashed line for unstimulated, dotted line for stimulated).
  • 12G9A36_AF647_IRF7_Antibody_FC_051414.jpg
    Overnight starved Jurkat cells were treated with or without IFN-α for 24 hours, then fixed and permeabilized with FOXP3 Fix/Perm Buffer Set. Cells were then stained with IRF7 (clone 12G9A36) Alexa Fluor® 647 (solid line with open histogram for untreated cells, filled histogram for stimulated cells) or mouse IgG2b, κ Alexa Fluor® 647 isotype control (dashed line for unstimulated, dotted line for stimulated).
Compare all formats See Alexa Fluor® 647 spectral data
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656007 25 tests £117
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656008 100 tests £278
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Description

IRF-7 is a member of the interferon regulatory transcription factor family that plays key regulatory roles in innate immunity. Upon virus infection, IRF-7 forms a complex with MyD88 and TRAF6 when stimulated by toll-like receptors TLR7, TLR8, and TLR9, which in turn results in nuclear translocation and induction of interferon gene expression. IRF-7 gene expression is upregulated by the stimulation of interferon through the Jak-STAT signaling pathway, which forms a positive feedback loop. The protein level of IRF-7 after activation is then downregulated by the ubiquitin-proteasome system to avoid excessive production of interferons that may cause damage to the host. Virus induced type I interferon expression was completely abrogated in IRF-7 knockout mice, suggesting that IRF-7 is critical for interferon mediated host defense during virus infection.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial IRF7 recombinant protein (127-503 a.a.)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

Product Citations
  1. Martínez-Fábregas J, et al. 2020. Cell Rep. 33:108545. PubMed
RRID
AB_2563531 (BioLegend Cat. No. 656007)
AB_2563531 (BioLegend Cat. No. 656008)

Antigen Details

Structure
503 amino acids, predicted molecular weight of 54 kD, contains an IRF tryptophan pentad repeat responsible for DNA binding
Distribution

Shuttles between the cytoplasm and nucleus; activated (phosphorylated) form of IRF-7 accumulates in the nucleus

Function
Transcription factor that transactivates proinflamatory cytokines and type I interferon genes in response to virus infection
Interaction
Forms homodimer or heterodimer with IRF-3, interacts with TICAM-1, TICAM-2, MyD88, and TRAF6
Cell Type
Dendritic cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction, Transcription Factors
Antigen References

1. Owens BM, et al. 2012. PLoS One. 7:e41050.
2. Yu Y, et al. 2010. Immunity. 33:863.
3. Liang Q, et al. 2011. J. Immunol. 186:1001.
4. Buss C, et al. 2010. J. Immunol. 184:3072.
5. Joo CH, et al. 2007. J. Virol. 81:8282.
6. Balachandran S, et al. 2007. J. Immunol. 178:2429.

Gene ID
3665 View all products for this Gene ID
UniProt
View information about IRF7 on UniProt.org
Go To Top Version: 2    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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