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Alexa Fluor® 647 anti-mouse CD268 (BAFF-R) Antibody

Pricing & Availability
Clone
7H22-E16 (See other available formats)
Regulatory Status
RUO
Other Names
BR3, BAFFR, BAFF receptor, TNFRSF13C, B cell-activating factor receptor, BLys receptor 3
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
A_7H22-E16_A647_062509.jpg
C57BL/6 splenocytes double stained with 7H22-E16 Alexa Fluor® 647 (upper panel) or rat IgG1 isotype control Alexa Fluor® 647 (lower panel) and B220 (RA3-6B2) PE
  • A_7H22-E16_A647_062509.jpg
    C57BL/6 splenocytes double stained with 7H22-E16 Alexa Fluor® 647 (upper panel) or rat IgG1 isotype control Alexa Fluor® 647 (lower panel) and B220 (RA3-6B2) PE
  • B_7H22-E16_A647_062509.jpg
Compare all formats See Alexa Fluor® 647 spectral data
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134105 25 µg £109
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134106 100 µg £217
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Description

BAFF (B cell-activating factor belonging to the TNF family) receptor (BAFF-R) is one of the three receptors that bind to BAFF. It is a type III transmembrane TNF receptor family member. BAFF-R is expressed at high levels in resting B cells and at lower levels in activated B cells, a small subset of activated/memory CD4+ T cells. It is a major mediator of BAFF dependent co-stimulatory response in peripheral B and T cells. It was reported that the mice expressing defective BAFF-R have disrupted B cell maturation. BAFF receptor appears to be particularly important for the regulation of normal B cell survival and maturation, as well as in the pathophysiology of aggressive B-cell malignancies and autoimmune diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse BAFF receptor transfected rat RBL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Ng LG, et al. 2006. Eur. J. Immunol. 36:1837.
  2. Ng LG, et al. 2004. J Immunol. 173:807.
  3. Batten M, et al. 2000. J. Exp. Med. 192:1453.
  4. Fu L, et al. 2009. Blood 113:4627.
Product Citations
  1. Zhou X, et al. 2013. Mol Cell Biol. 33:2091. PubMed
RRID
AB_2203551 (BioLegend Cat. No. 134105)
AB_2203551 (BioLegend Cat. No. 134106)

Antigen Details

Structure
A type III transmembrane TNF receptor family member
Distribution

Expressed on most B cells, a small subset of CD4+ T cells.

Function
Play an importance role in B cell development, maturation, and survival.
Ligand/Receptor
BAFF (BLyS)
Cell Type
B cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Thompson JS, et al. 2001. Science 293:2108.
2. Schiemann B, et al. 2001. Science 293:2111.
3. Batten M, et al. 2000. J. Exp. Med. 192:1453.
4. Fu L, et al. 2009. Blood 113:4627.

Gene ID
72049 View all products for this Gene ID
UniProt
View information about CD268 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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