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Alexa Fluor® 647 anti-mouse CD301a (MGL1) Antibody

Pricing & Availability
Clone
LOM-8.7 (See other available formats)
Regulatory Status
RUO
Other Names
MGL1, MGL, M-ASGP-BP, Macrophage galactose-type C-type lectin, CLEC10A
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
LOM-8.7_AF647_CD301a_Antibody_FC_1_040213
C57BL/6 bone marrow-derived dendritic cells were stained with CD11c PE and CD301a (clone LOM-8.7) Alexa Fluor® 647 (top) or rat IgG2a, κ Alexa Fluor® 647 isotype control (bottom).
  • LOM-8.7_AF647_CD301a_Antibody_FC_1_040213
    C57BL/6 bone marrow-derived dendritic cells were stained with CD11c PE and CD301a (clone LOM-8.7) Alexa Fluor® 647 (top) or rat IgG2a, κ Alexa Fluor® 647 isotype control (bottom).
  • LOM-8.7_AF647_CD301a_Antibody_FC_2_040213
Compare all formats See Alexa Fluor® 647 spectral data
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145603 25 µg £121
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145604 100 µg £262
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Description

Mouse CD301, also known as macrophage galactose-type C-type lectin, has two homologue genes, CD301a (MGL1) and CD301b (MGL2), while there is only one MGL in human and rat.  Mouse CD301a and CD301b are ~42 kD type II transmembrane glycoproteins containing a cytoplasmic domain, a transmembrane domain, a neck domain, and a carbohydrate recognition domain (CRD) within each molecule. CD301a is mainly expressed on a subset of macrophages and immature dendritic cells (DCs). CD301b is mainly found on conventional DCs. Although CD301a and CD301b share high amino acid sequence homology (92% for the intact sequence and 80% for the CRD), they display different carbohydrate specificities. CD301a was found to be highly specific for Lewis X and Lewis A structures, whereas CD301b, more similar to the human MGL, recognizes N-actetylgalactosamine (GalNAc) and galactose, including the O-linked Tn-antigen, TF-antigen, and core 2.  So far, CD301a has been reported to be involved in recognition and  endocytosis of glycoproteins with terminal Gal/GalNAc moieties. This contributes to defense against tumor cell metastasis, tissue remodeling, and clearance of apoptotic cells in embryos. CD301b is involved in the internalization of soluble polyacrylamide polymers (PAA) with α-GalNAc residues (GalNAc-PAA) in bone marrow derived dendritic cells. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Purified and recombinant mouse MGL1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

LOM-8.7 specifically recognizes mouse CD301a. Additional reported applications (for relevant formats) include: immunohistochemical staining of frozen tissue sections1-3, immunoprecipitation4, and blocking of MGL1 binding to its ligand4.

Application References

(PubMed link indicates BioLegend citation)
  1. Denda-Nagai K, et al. 2010. J. Biol. Chem. 285:19193. (IHC)
  2. Sato K, et al. 2005. Blood 106:207. (IHC)
  3. Tsuiji M, et al. 2002. J. Biol. Chem. 277:28892. (IHC)
  4. Kimura T, et al. 1995. J. Biol. Chem. 270:16056. (IP, Block)
Product Citations
  1. Morris CR, et al. 2022. Mucosal Immunol. 15:977. PubMed
RRID
AB_2561987 (BioLegend Cat. No. 145603)
AB_2561987 (BioLegend Cat. No. 145604)

Antigen Details

Structure
42 kD type II transmembrane glycoprotein, containing a cytoplasmic domain, a transmembrane domain, a neck domain, and a carbohydrate recognition domain (CRD) within each molecule
Distribution

Immature myeloid dendritic cells, a subset of macrophages

Function
Cell adhesion, cell-cell signaling, glycoprotein turnover, inflammation, tissue remodeling
Interaction
Carbohydrate determinants, GP envelope glycoprotein on Marburg and Ebola viruses
Ligand/Receptor
Sialoadhesin, apoptotic cells, and commensal bacteria such as Streptococcus sp
Cell Type
Dendritic cells, Macrophages
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Denda-Nagai K, et al. 2010. J. Biol. Chem. 285:19193.
2. Westcott D, et al. 2009. J. Exp. Med. 206:3143.
3. Singh SK, et al. 2008. Mol. Immunol. 46:1240.
4. Sakakura M, et al. 2008. J. Biol. Chem. 283:33665.
5. Tsuiji M, et al. 2002. J. Biol. Chem. 277:28892.

Gene ID
17312 View all products for this Gene ID
UniProt
View information about CD301a on UniProt.org
Go To Top Version: 2    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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