Purified anti-mouse CD301a MGL1 Antibody anti-CD301a

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Clone: LOM-8.7 (See other available formats)
Regulatory Status: RUO
Other Names: MGL1, MGL, M-ASGP-BP, Macrophage galactose-type C-type lectin, CLEC10A
Isotype: Rat IgG2a, κ
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LOM-8.7_Purified_CD301a_Antibody_FC_1_032613 — C57BL/6 bone marrow-derived dendritic cells were stained with CD11c APC and purified CD301a (MGL1) (clone LOM-8.7) (top) or purified rat IgG2a, κ (bottom), followed by anti-rat IgG PE.

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145602

100 µg

£177

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Description

Mouse CD301, also known as macrophage galactose-type C-type lectin, has two homologue genes, CD301a (MGL1) and CD301b (MGL2), while there is only one MGL in human and rat. Mouse CD301a and CD301b are ~42 kD type II transmembrane glycoproteins containing a cytoplasmic domain, a transmembrane domain, a neck domain, and a carbohydrate recognition domain (CRD) within each molecule. CD301a is mainly expressed on a subset of macrophages and immature dendritic cells (DCs). CD301b is mainly found on conventional DCs. Although CD301a and CD301b share high amino acid sequence homology (92% for the intact sequence and 80% for the CRD), they display different carbohydrate specificities. CD301a was found to be highly specific for Lewis X and Lewis A structures, whereas CD301b, more similar to the human MGL, recognizes N-actetylgalactosamine (GalNAc) and galactose, including the O-linked Tn-antigen, TF-antigen, and core 2. So far, CD301a has been reported to be involved in recognition and endocytosis of glycoproteins with terminal Gal/GalNAc moieties. This contributes to defense against tumor cell metastasis, tissue remodeling, and clearance of apoptotic cells in embryos. CD301b is involved in the internalization of soluble polyacrylamide polymers (PAA) with α-GalNAc residues (GalNAc-PAA) in bone marrow derived dendritic cells.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Purified and recombinant mouse MGL1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: LOM-8.7 specifically recognizes mouse CD301a. Additional reported applications (for relevant formats) include: immunohistochemical staining of frozen tissue sections^1-3, immunoprecipitation⁴, and blocking of MGL1 binding to its ligand⁴.
RRID: AB_2561958 (BioLegend Cat. No. 145602)

Antigen Details

Structure: 42 kD type II transmembrane glycoprotein, containing a cytoplasmic domain, a transmembrane domain, a neck domain, and a carbohydrate recognition domain (CRD) within each molecule
Distribution: Immature myeloid dendritic cells, a subset of macrophages
Function: Cell adhesion, cell-cell signaling, glycoprotein turnover, inflammation, tissue remodeling
Interaction: Carbohydrate determinants, GP envelope glycoprotein on Marburg and Ebola viruses
Ligand/Receptor: Sialoadhesin, apoptotic cells, and commensal bacteria such as Streptococcus sp
Cell Type: Dendritic cells, Macrophages
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Denda-Nagai K, et al. 2010. J. Biol. Chem. 285:19193.
2. Westcott D, et al. 2009. J. Exp. Med. 206:3143.
3. Singh SK, et al. 2008. Mol. Immunol. 46:1240.
4. Sakakura M, et al. 2008. J. Biol. Chem. 283:33665.
5. Tsuiji M, et al. 2002. J. Biol. Chem. 277:28892.
Gene ID: 17312 View all products for this Gene ID
UniProt: View information about CD301a on UniProt.org

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Other Formats

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Description	Clone	Applications
Purified anti-mouse CD301a (MGL1)	LOM-8.7	FC,IHC-F,IP
Alexa Fluor® 647 anti-mouse CD301a (MGL1)	LOM-8.7	FC
PE anti-mouse CD301a (MGL1)	LOM-8.7	FC
PE/Cyanine7 anti-mouse CD301a (MGL1)	LOM-8.7	FC
TotalSeq™-A0551 anti-mouse CD301a (MGL1)	LOM-8.7	PG
TotalSeq™-B0551 anti-mouse CD301a (MGL1)	LOM-8.7	PG

Certificate of Analysis

Go To Top Version: 1 Revision Date: 04/05/2013

For Research Use Only. Not for diagnostic or therapeutic use.

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