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Alexa Fluor® 647 anti-Phosphothreonine Antibody

Pricing & Availability
Clone
M380A (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
M380A_A-647_Phosphothreonine_ICFC_072224
PBMCs either untreated (negative control, open histogram) or treated for with Cell Activation Cocktail (without Brefeldin A)(Cat. No. 423301) (positive control, filled histogram) were fixed and permeabilized using True Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with Alexa Fluor® 647 anti-Phosphothreonine (clone M380A), or Alexa Fluor® 647 anti-mouse IgG1, κ isotype control (Cat. No. 400136) (Open histogram, dashed line is representative for both, untreated and treated PBMC).
  • M380A_A-647_Phosphothreonine_ICFC_072224
    PBMCs either untreated (negative control, open histogram) or treated for with Cell Activation Cocktail (without Brefeldin A)(Cat. No. 423301) (positive control, filled histogram) were fixed and permeabilized using True Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with Alexa Fluor® 647 anti-Phosphothreonine (clone M380A), or Alexa Fluor® 647 anti-mouse IgG1, κ isotype control (Cat. No. 400136) (Open histogram, dashed line is representative for both, untreated and treated PBMC).
Compare all formats See Alexa Fluor® 647 spectral data
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943005 25 tests £120
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943006 100 tests £301
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Description

Phosphorylation of specific threonine residues is a post-translational modification event critical for regulating the activity of many proteins.  Protein phosphorylation events can alter protein function through a variety of molecular mechanisms, ranging from conformational changes that can stimulate or inhibit protein function to facilitating protein-protein interactions with binding partners. Antibodies that can detect phosphothreonine residues are valuable tools for identifying novel phospho-sites and characterizing changes in the post-translational state of a broad range of phosphorylated proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide containing phosphorylated threonine
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

For use of this antibody (clone M380A) in intracellular staining for flow cytometry (ICFC), we recommend using True-Phos ™ Perm buffer set (Cat. No. 425401) for fixation/permeabilization.

RRID
AB_3662344 (BioLegend Cat. No. 943005)
AB_3662344 (BioLegend Cat. No. 943006)

Antigen Details

Function
Cell signaling
Antigen References

1. Zhao et. al. 2016. Sci. Reports. 6:34817.
2. De Verdier C. H. 1952. Nature. 170:804-805.
3. Steinfield J. et. al. 2014. ACS Chem. Biol. 9(5):1104-1112.
4. Mora N. et. al. 2009. Int. Journ. of Peptide and Protein Research. 45(1):53-63.

Gene ID
NA
UniProt
View information about Phosphothreonine on UniProt.org
Go To Top Version: 1    Revision Date: 07/22/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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