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PE anti-Phosphothreonine Antibody

Pricing & Availability
Clone
M380A (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
M380A_PE_Phosphothreonine_Antibody_031224
PBMCs untreated (negative control, open histogram) or treated with Cell Activation Cocktail (Cat. No. 423301) (positive control, solid histogram) were fixed and permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with PE anti-Phosphothreonine (clone M380A), or PE anti-Mouse IgG1, κ isotype control (open histogram, dashed line is representative for both, untreated and treated cells) (Cat No. 400140).
  • M380A_PE_Phosphothreonine_Antibody_031224
    PBMCs untreated (negative control, open histogram) or treated with Cell Activation Cocktail (Cat. No. 423301) (positive control, solid histogram) were fixed and permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with PE anti-Phosphothreonine (clone M380A), or PE anti-Mouse IgG1, κ isotype control (open histogram, dashed line is representative for both, untreated and treated cells) (Cat No. 400140).
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943003 25 tests £117
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943004 100 tests £250
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Description

Phosphorylation of specific threonine residues is a post-translational modification event critical for regulating the activity of many proteins.  Protein phosphorylation events can alter protein function through a variety of molecular mechanisms, ranging from conformational changes that can stimulate or inhibit protein function to facilitating protein-protein interactions with binding partners. Antibodies that can detect phosphothreonine residues are valuable tools for identifying novel phospho-sites and characterizing changes in the post-translational state of a broad range of phosphorylated proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide containing phosphorylated threonine
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_3097636 (BioLegend Cat. No. 943003)
AB_3097636 (BioLegend Cat. No. 943004)

Antigen Details

Function
Cell signaling
Antigen References

1. Zhao et. al. 2016. Sci. Reports. 6:34817.
2. De Verdier C. H. 1952. Nature. 170:804-805.
3. Steinfield J. et. al. 2014. ACS Chem. Biol. 9(5):1104-1112.
4. Mora N. et. al. 2009. Int. Journ. of Peptide and Protein Research. 45(1):53-63.

Gene ID
NA
UniProt
View information about Phosphothreonine on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 03/12/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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