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Alexa Fluor® 647 anti-rat CD103 (αE Integrin) Antibody

Pricing & Availability
Clone
OX-62 (See other available formats)
Regulatory Status
RUO
Other Names
Integrin αE, integrin E, integrin αIEL, integrin αOX-62, Itgae
Isotype
Mouse IgG1
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Product Citations
publications
OX-62_AF647_1_111710
LOU rat intestinal intraepithelial lymphocytes were stained with CD3 FITC and CD103 (clone OX-62) Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 isotype control (bottom).
  • OX-62_AF647_1_111710
    LOU rat intestinal intraepithelial lymphocytes were stained with CD3 FITC and CD103 (clone OX-62) Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 isotype control (bottom).
  • OX-62_AF647_2_111710
Compare all formats See Alexa Fluor® 647 spectral data
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205509 100 µg £150
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Description

CD103, also known as Integrin αE or integrin αIEL, is a single pass transmembrane protein composed by two chains, heavy and light, linked by a disulfide bond. It is expressed in the cell surface as an αE/β7 heterodimer. CD103 mediates adhesion of intraepithelial T cells (IEL) to the epithelium. CD103 is expressed by IEL, effector and memory Tregs, and dendritic cells and their immediate precursors. E-cadherin is the ligand for CD103.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Density gradient enriched lymph veiled cells from PVG rats
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen skin and intestine epithelium1,4, immunocytochemical staining of cytospin preparations4, and Western blot1.

Application References

(PubMed link indicates BioLegend citation)
  1. Brenan M, et al. 1992. J. Exp. Med. 175:1457. (IHC, WB)
  2. Chen-Woan M, et al. 1995. J. Immunol. Methods 178:157.
  3. Chen-Woan M, et al. 1996. J. Leuk. Biol. 59:196.
  4. Brenan M and Rees DJ. 2000. Eur. J. Immunol. 30:1527. (IHC)
Product Citations
  1. Wu H, et al. 2016. Sci Rep. 6: 24548. PubMed
RRID
AB_10682895 (BioLegend Cat. No. 205509)

Antigen Details

Structure
Single pass transmembrane protein, composed by two chains, heavy and light, linked by a disulfide bond. Associates with integrin β7 to form the αE/β7 complex.
Distribution

Intraepithelial T lymphocytes, effector and memory Tregs, dendritic cells and their immediate precursors.

Function
Mediates adhesion of intraepithelial T cells (IEL) to epithelium.
Ligand/Receptor
E-cadherin
Cell Type
Dendritic cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Varas A, et al. 1998. Blood 92:93.
2. Brissette-Storkus CS, et al. 2002. J. Leukoc. Biol. 71:941.
3. Hubert FX, et al. 2004. J. Immunol. 172:7485.
4. Tsuchihashi S, et al. 2006. J. Immunol. 176:616.
5. Lührmann A, et al. 2007. Immunol. Lett. 108:167.

Gene ID
83577 View all products for this Gene ID
UniProt
View information about CD103 on UniProt.org
Go To Top Version: 2    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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