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Alexa Fluor® 647 anti-ZAP70 Antibody

Pricing & Availability
Clone
A15114B (See other available formats)
Regulatory Status
RUO
Other Names
Zeta chain associated protein kinase, SYK related tyrosine kinase, ZAP-70
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A15114B_A647_ZAP70_Antibody_1_010218
Human peripheral blood lymphocytes were fixed with Fixation Buffer (Cat No. 420801), permeabilized with cold True-Phos™ Perm Buffer (Cat No. 425401), then stained with CD3 Alexa Fluor® 488 and anti-ZAP70 (clone A15114B) Alexa Fluor® 647 (top) or mouse IgG2b, κ PE (Bottom).
  • A15114B_A647_ZAP70_Antibody_1_010218
    Human peripheral blood lymphocytes were fixed with Fixation Buffer (Cat No. 420801), permeabilized with cold True-Phos™ Perm Buffer (Cat No. 425401), then stained with CD3 Alexa Fluor® 488 and anti-ZAP70 (clone A15114B) Alexa Fluor® 647 (top) or mouse IgG2b, κ PE (Bottom).
  • A15114B_A647_ZAP70_Antibody_2_010218
Compare all formats See Alexa Fluor® 647 spectral data
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691205 25 tests £94
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691206 100 tests £238
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Description

ZAP70 is a 70 kD protein tyrosine kinase associated with the zeta chain of the T cell receptor. It is expressed in T cells and NK cells and has been shown to be involved in T cell signaling. Defects in ZAP70 have been linked to selective T cell defects. The ZAP70 kinase undergoes multiple phosphorylation events after T cell receptor engagement and interacts with a number of proteins involved in signal transduction. Recently, ZAP70 has been identified as an important prognostic marker in B-cell chronic lymphocytic leukemia (B-CLL).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH-peptide surrounding ZAP70 Y319.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
RRID
AB_2721438 (BioLegend Cat. No. 691205)
AB_2721438 (BioLegend Cat. No. 691206)

Antigen Details

Structure
Protein tyrosine kinase, contains two SH2 domains. Human Zap70 has three isoforms with predicted MW of 70kD, 56kD, 36kD. Mouse Zap70 has three isoforms with predicted MW of 70kD, 36kD, 35kD.
Distribution

T cells, NK cells.

Function
Signal transduction, associates with the T-cell antigen receptor zeta chain tyrosine-based activation motif when phosphorylated. Defects in ZAP70 causes selective T cell defect.
Interaction
Signal transduction, associates with the T-cell antigen receptor zeta chain tyrosine-based activation motif when phosphorylated.
Cell Type
NK cells, T cells
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase, TCRs
Antigen References

1. Chan AC, et al. 1992. Cell. 71:649.
2. Chan AC, et al. 1994. Science. 264:1599.
3. Arpaia E, et al. 1994. Cell. 76:947.

Gene ID
7535 View all products for this Gene ID
UniProt
View information about ZAP70 on UniProt.org
Go To Top Version: 1    Revision Date: 01/03/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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