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Alexa Fluor® 647 Donkey anti-rabbit IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4064 (See other available formats)
Regulatory Status
RUO
Isotype
Donkey Polyclonal Ig
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Product Citations
publications
Poly4064_AF647_IgG_ANtibody_FC_022014.jpg
C57/BL6 splenocytes were stained with NK-1.1 Brilliant Violet 421™ and purified rabbit anti-Asialo-GM1, followed by donkey anti-rabbit IgG (clone Poly4064) Alexa Fluor® 647.
  • Poly4064_AF647_IgG_ANtibody_FC_022014.jpg
    C57/BL6 splenocytes were stained with NK-1.1 Brilliant Violet 421™ and purified rabbit anti-Asialo-GM1, followed by donkey anti-rabbit IgG (clone Poly4064) Alexa Fluor® 647.
Compare all formats See Alexa Fluor® 647 spectral data
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406414 100 µg £97
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Description

This polyclonal donkey anti-rabbit IgG antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other subclasses of rabbit immunoglobulins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rabbit
Antibody Type
Polyclonal
Host Species
Donkey
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

This conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.

Product Citations
  1. Guo H, et al. 2021. Nat Immunol. 22:423. PubMed
  2. Schneider J, et al. 2022. EMBO J. 41:e110409. PubMed
  3. Madi A, et al. 2022. Int J Cancer. 151:797. PubMed
  4. Clifton C, et al. 2022. Front Cell Infect Microbiol. 12:1035566. PubMed
  5. Huang Z, et al. 2022. Cell Rep. 39:110575. PubMed
  6. Wang JX, et al. 2023. JCI Insight. 8:. PubMed
  7. Okano F, et al. 2023. Cancer Res Commun. 3:640. PubMed
  8. Song F, et al. 2021. Ann Transl Med. 9:1374. PubMed
  9. Meryk A et al. 2019. Cell reports. 26(10):2681-2691 . PubMed
  10. Sun Z, et al. 2021. eLife. 10:00. PubMed
  11. Mukherjee T, et al. 2022. Autophagy. 18:391. PubMed
  12. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  13. Yadav P, et al. 2021. J Immunol. 206:2888. PubMed
  14. Lu Q, et al. 2021. Immunity. 54(6):1304-1319.e9. PubMed
  15. Tiwarekar V, et al. 2019. Viruses. 11:. PubMed
  16. Niemeyer BF, et al. 2021. Nano Sel. . PubMed
  17. Gagnon JD, et al. 2019. Cell Rep. 28:2169. PubMed
  18. Kyle J, et al. 2019. Front Cell Neurosci. 13:97. PubMed
  19. von Furstenberg RJ, et al. 2017. Cell Mol Gastroenterol Hepatol. 0.434027778. PubMed
  20. Lauridsen H, et al. 2017. FASEB J. 10.1016/j.ajpath.2017.04.008. PubMed
  21. So L, et al. 2016. Sci Signal. 9: ra57. PubMed
  22. Daneshmandi S, et al. 2021. Cell Reports. 34(10):108831. PubMed
  23. Mead BE et al. 2018. BMC biology. 16(1):62 . PubMed
  24. Vickman RE, et al. 2019. Mol Cancer Res. 17:1253. PubMed
  25. Lee GR, et al. 2021. JCI Insight. 6:. PubMed
  26. Guo X, et al. 2021. iScience. 24:103295. PubMed
RRID
AB_2563202 (BioLegend Cat. No. 406414)

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Go To Top Version: 3    Revision Date: 02/09/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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