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Cyanine3 Donkey anti-rabbit IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4064 (See other available formats)
Regulatory Status
RUO
Isotype
Donkey Polyclonal Ig
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Product Citations
publications
Poly4064_Cyanine3_IgG_Antibody_050520
HeLa cells were fixed with ice cold methanol for six minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 1 µg/mL of purified anti-Giantin (clone Poly19087) and followed by Cyanine3 Donkey anti-rabbit IgG (clone Poly4064) antibody (red) for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
  • Poly4064_Cyanine3_IgG_Antibody_050520
    HeLa cells were fixed with ice cold methanol for six minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 1 µg/mL of purified anti-Giantin (clone Poly19087) and followed by Cyanine3 Donkey anti-rabbit IgG (clone Poly4064) antibody (red) for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
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406402 100 µg £73
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Description

This polyclonal donkey anti-rabbit IgG antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other subclasses of rabbit immunoglobulins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rabbit
Antibody Type
Polyclonal
Host Species
Donkey
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with Cyanine3 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
Recommended Usage

It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.

Product Citations
  1. Liu J, et al. 2019. Mol Med Rep. 20:33. PubMed
  2. Zenker S, et al. 2014. J Immunol. 192:2830. PubMed
  3. Meinen S, et al. 2012. PLoS One. 7:e44148. PubMed
  4. Sun ZC, et al. 2021. Front Immunol. 12:762162. PubMed
  5. Rauner G, Barash I 2014. Exp Cell Res. 328:186. PubMed
  6. Noh KH, et al. 2022. Transl Oncol. 15:101255. PubMed
  7. Ali H, et al. 2014. J Control Release. 183:167. PubMed
  8. Shibuya T, et al. 2021. Front Immunol. 12:663115. PubMed
  9. Han JK, et al. 2021. eLife. 10:00. PubMed
  10. Hu J, et al. 2017. Cell Death Dis. 10.1038/cddis.2017.72. PubMed
RRID
AB_893532 (BioLegend Cat. No. 406402)

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Go To Top Version: 4    Revision Date: 05/05/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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