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Alexa Fluor® 700 anti-human CD18 Antibody

Pricing & Availability
Clone
TS1/18 (See other available formats)
Regulatory Status
RUO
Workshop
V AS162
Other Names
Integrin β2 subunit, LFA -1 β subunit, β2 integrin, ITGB2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
TS1slash18_A700_CD18_Antibody_091418
Human peripheral blood lymphocytes were stained with CD18 (clone TS1/18) Alexa Fluor® 700 (filled histogram) or Mouse IgG1, κ Alexa Fluor® 700 isotype control (open histogram).
  • TS1slash18_A700_CD18_Antibody_091418
    Human peripheral blood lymphocytes were stained with CD18 (clone TS1/18) Alexa Fluor® 700 (filled histogram) or Mouse IgG1, κ Alexa Fluor® 700 isotype control (open histogram).
Compare all formats See Alexa Fluor® 700 spectral data
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302123 25 tests £109
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302124 100 tests £238
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Description

CD18 is a 90-95 kD type I transmembrane protein also known as integrin β2 subunit, LFA-1 β subunit, and β2 integrin. CD18 non-covalently associates with CD11a, CD11b or CD11c. CD18 is expressed on all leukocytes. CD18 and associated α chains function in adhesion and signaling in hematopoietic cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Pig
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: inhibition of cell adhesion and migration3,4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 302116) is recommended for functional assays.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Kishimoto T, et al. 1997. Leucocyte Typing VI. Garland Press. London.
  3. Van Epps DE, et al. 1989. J. Immunol. 143:3207. (Block)
  4. Meerschaert J, et al. 1994. J. Immunol. 152:1915. (Block)
  5. Sithu SD, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M611273200.
  6. Sommaggio R, et al. 2012. J. Immunol. 188:2075. PubMed
  7. Valenzuela NM, et al. 2013. J. Immunol. 190:6635. PubMed
Product Citations
  1. Reif T, et al. 2021. Cell Reports Medicine. 2(6):100317. PubMed
RRID
AB_2750394 (BioLegend Cat. No. 302123)
AB_2750394 (BioLegend Cat. No. 302124)

Antigen Details

Structure
Integrin, type I transmembrane glycoprotein, associates with CD11a, CD11b, or CD11c, 90-95 kD
Distribution

Leukocytes

Function
Adhesion and signaling in hematopoietic system
Ligand/Receptor
ICAMs
Cell Type
Leukocytes, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Anderson D, et al. 1987. Annu. Rev. Med. 38:175.
2. Springer T. 1994. Cell 76:301.

Gene ID
3689 View all products for this Gene ID
UniProt
View information about CD18 on UniProt.org
Go To Top Version: 1    Revision Date: 09/18/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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