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Anti-MAP2 Antibody (Previously Covance catalog# PRB-547C)

Pricing & Availability
Clone
Poly18406 (See other available formats)
Regulatory Status
RUO
Other Names
Microtubule-Associated Protein 2, Map-2
Previously
Covance Catalog# PRB-547C
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly18406_Serum_MAP2_Antibody_031521
Figure Legend: IHC staining of purified anti-MAP2 antibody (Poly18406) on formalin-fixed, paraffin-embedded mouse brain. The tissue was incubated with primary antibody (1:2000 dilution) for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929501) was used for detection followed by hemotoxylin counterstaining, according to the protocol provided.
  • Poly18406_Serum_MAP2_Antibody_031521
    Figure Legend: IHC staining of purified anti-MAP2 antibody (Poly18406) on formalin-fixed, paraffin-embedded mouse brain. The tissue was incubated with primary antibody (1:2000 dilution) for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929501) was used for detection followed by hemotoxylin counterstaining, according to the protocol provided.
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840601 100 µL £257
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Description

Microtubules are required for many well characterized functions in eukaryotic cells, including the movement of chromosomes in mitosis and meiosis, intracellular transport, establishment and maintenance of cellular morphology, cell growth, cell migration and morphogenesis in multicellular organisms. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which include the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two 280 kD apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at 70 kD on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Reported Reactivity
Other species
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
The MAP2 antibody was raised using bovine brain MAP2, electrophoretically purified from heat stable MAP fraction and recognizes mammalian MAP2.
Preparation
Serum
Storage & Handling
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Application

IHC-P - Quality tested
ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a dilution range of 1:1000 - 1:2000 is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB), immunofluorescence (IF) and immunohistochemistry (IHC).

Positive control: Mouse brain

The antibody will stain neuronal cell bodies and dendritic processes, and will not stain glial cells or axons. It is the best marker to distinguish dendrites from axons in the brain.

This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. De Camilli P, et al. 1984. Neuroscience. 11:817-46. (IHC)
  2. Bloom GS, et al. 1984. J Cell Biol. 98:320-30. (IHC)
  3. Maschietto M, et al. 2013. Front Cell Neurosci. 25:7. (IF) PubMed
  4. La Via L,et al. 2013. Nucelic Acids Res. 41:617. (IF) PubMed
  5. Ji B, et al. 2014. Hum Mol Genet. 23:5683-705. (ICC) PubMed
  6. Fu X, et al. 2013. J Neurosci. 33:709.
Product Citations
  1. Karacosta LG, et al. 2019. Nat Commun. 5587:10. PubMed
  2. Luo PW, et al. 2019. Adv Biosyst. e1800254:3. PubMed
  3. Castro VIB, et al. 2023. ACS Appl Mater Interfaces. 29998:15. PubMed
  4. Ji B, et al. 2014. Hum Mol Genet. 23:5683-5705. PubMed
  5. Maschietto M, et al. 2013. Front Cell Neurosci. 7:2. PubMed
  6. Via L, et al. 2013. Nucleic Acids Res. 41:617-631. PubMed
  7. Kawashima T, et al. 2021. Journal of Neurochemistry. 157(4):1207-1221. PubMed
  8. Zhou X, et al. 2020. International Journal of Molecular Sciences. 21(8):2841.. PubMed
  9. Cai T, et al. 2021. Cell Reports. 36(2):109337. PubMed
RRID
AB_2565455 (BioLegend Cat. No. 840601)

Antigen Details

Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Microtubules
Gene ID
4133 View all products for this Gene ID
UniProt
View information about MAP2 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All MAP2 Reagents Request Custom Conjugation
Description Clone Applications
Anti-MAP2 Poly18406 IHC-P,ICC
Go To Top Version: 5    Revision Date: 09/22/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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