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APC anti-human CD158d (KIR2DL4) Antibody

Pricing & Availability
Clone
mAb 33 (33) (See other available formats)
Regulatory Status
RUO
Other Names
KIR2DL4, 2DL4, p49, KIR103
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
33_158d_APC_top_04271
IL-2 stimulated (18hrs) human peripheral blood lymphocytes stained with CD56 FITC and mAb 33 APC (top) or mouse IgG1,κ APC isotype control (bottom)
  • 33_158d_APC_top_04271
    IL-2 stimulated (18hrs) human peripheral blood lymphocytes stained with CD56 FITC and mAb 33 APC (top) or mouse IgG1,κ APC isotype control (bottom)
  • 33_158d_APC_zbottom_042710
    IL-2 stimulated (18hrs) human peripheral blood lymphocytes stained with CD56 FITC and mAb 33 APC (top) or mouse IgG1,κ APC isotype control (bottom)
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347007 25 tests £106
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347008 100 tests £248
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Description

CD158 molecules, also known as KIRs (killer cell immunoglobulin-like receptors), are a family of transmembrane proteins with either two (KIR2D) or three (KIR3D) Ig-like extracellular domains. Some KIRs, with long cytoplasmic domains, contain ITIMs and possess inhibitory functions and others, with short cytoplasmic regions, lack ITIM and have activation functions. Fourteen polymorphic KIR genes have been reported in humans. KIR2DL4 (CD158d) is a unique receptor which has an ITIM in its cytoplasmic domain and a charged residue in the transmembrane domain. It possesses both inhibitory and activation functions. Two common alleles (10A and 9A) of KIR2DL4 have been reported. The 10A allele (with 10 adenines at the end of the transmembrane exon) receptor is expressed on CD56high NK subset, whereas its expression on CD56dim NK cells is inducible upon culture. The major 9A allele receptor is a secreted form. HLA-G is the ligand of CD158d.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NK3.3 cells and KIR2DL4-Ig fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

KIR2DL4 expression in NK cells is highly localized to the endosomes3. mAb 33 can be internalized by NK cells by endocytosis at 37°C3, and induces NK cell cytotoxicity1,2. Based on in-house observations, we suggest incubating for 45 - 60 minutes at room temperature with clone mAb 33 to observe better CD158d staining.

This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of paraffin-embedded tissue sections (IHC-P).

Application References

(PubMed link indicates BioLegend citation)
  1. Rajagopalan S, et al. 2001. J. Immunol. 167:1877. (FC)
  2. Goodridge JP, et al. 2003. J. Immunol. 171:1768. (FC)
  3. Rajagopalan S, et al. 2006. PLoS Biol. 4:e9.
Product Citations
  1. Brusilovsky M, et al. 2013. J Immunol. 191:5256. PubMed
  2. Fu B et al. 2017. Immunity. 47(6):1100-1113 . PubMed
RRID
AB_2130691 (BioLegend Cat. No. 347007)
AB_2130691 (BioLegend Cat. No. 347008)

Antigen Details

Structure
Contains two Ig-like extracellular domains, 45-50 kD
Distribution

On CD56high NK cells and cultured CD56dim NK cells

Ligand/Receptor
HLA-G
Cell Type
NK cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Rajagopalan S, et al. 2006. PLoS Biol. 4:e9.
2. Goodridge JP, et al. 2007. Eur. J. Immunol. 37:199.

Gene ID
3805 View all products for this Gene ID
UniProt
View information about CD158d on UniProt.org
Go To Top Version: 2    Revision Date: 05/04/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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