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APC anti-human CD18 Antibody

Pricing & Availability
Clone
1B4/CD18 (See other available formats)
Regulatory Status
RUO
Other Names
Integrin beta-2, integrin beta chain, beta 2
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
1B4slashCD18_APC_CD18_Antibody_082517
Human peripheral blood lymphocytes were stained with CD18 (clone 1B4/CD18) APC (filled histogram) or mouse IgG2a, κ APC isotype control (open histogram).
  • 1B4slashCD18_APC_CD18_Antibody_082517
    Human peripheral blood lymphocytes were stained with CD18 (clone 1B4/CD18) APC (filled histogram) or mouse IgG2a, κ APC isotype control (open histogram).
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373405 25 tests £86
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373406 100 tests £208
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Description

CD18 is a 90-95 kD type I transmembrane protein also known as integrin β2 subunit, LFA-1 β subunit, and β2 integrin. CD18 non-covalently associates with CD11a, CD11b or CD11c. CD18 is expressed on all leukocytes. CD18 and associated α chains function in adhesion and signaling in hematopoietic cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human peripheral blood lymphocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

The 1B4/CD18 antibody was able to partially block the Ts1/18 antibody, indicating the two anti-CD18 antibodies recognize distinct but overlapping epitopes. 

Application References

(PubMed link indicates BioLegend citation)
  1. Singer II, et al. 1993. J. Immunol. 150:2844.
  2. Seth R, et al. 1991. FEBS Lett. 282:193.
Product Citations
  1. Zhang W, et al. 2022. Dev Cell. 57:329. PubMed
  2. Velázquez–Avila M, et al. 2019. Leukemia. 33:1337. PubMed
RRID
AB_2716022 (BioLegend Cat. No. 373405)
AB_2716022 (BioLegend Cat. No. 373406)

Antigen Details

Structure
Integrin, type I transmembrane glycoprotein
Distribution

Leukocytes

Function
Adhesion and signaling in hematopoietic cells
Interaction
Associates with CD11a, CD11b, or CD11c
Ligand/Receptor
ICAMs
Cell Type
Leukocytes, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Hogg N, et al. 1999. J. Clin. Invest. 103:97.
2. Anderson DC, et al. 1987. Annu. Rev. Med. 38:175.
3. Springer TA. 1994. Cell 76:301.

Gene ID
3689 View all products for this Gene ID
UniProt
View information about CD18 on UniProt.org
Go To Top Version: 0    Revision Date: 08/28/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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