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APC anti-human CD49c (integrin α3) Antibody

Pricing & Availability
Clone
ASC-1 (See other available formats)
Regulatory Status
RUO
Workshop
VI A002
Other Names
α3 integrin, integrin α3 chain, VLA-3 α chain, VLA3a, ITGA3
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
ASC-1_APC_081910
Human cervical cancer cell line HeLa cells stained with ASC-1 APC
  • ASC-1_APC_081910
    Human cervical cancer cell line HeLa cells stained with ASC-1 APC
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343808 100 tests £215
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Description

CD49c is a 150 kD α integrin chain known as α3 integrin or VLA-3 α chain. It is a type I transmembrane glycoprotein which is proteolytically cleaved into two disulfide linked fragments of 125 kD and 30 kD. CD49c forms a heterodimer with integrin β1 (α3β1, CD49c/CD29, VLA-3) and is expressed by many types of adhesion cells, such as endothelial cells, epithelial cells, and dermal fibroblasts. Weak expression has been reported on leukocytes. VLA-3 plays a role in cell-cell and cell-matrix adhesion through binding Kalinin, collagen, laminin-1, laminin-5, entactin, and fibronectin.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human squamous cell carcinoma cell line SCC-9
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications include: immunoprecipitation4, Western blotting5, and immunohistochemical staining of frozen tissue sections2.

Application References

(PubMed link indicates BioLegend citation)
  1. Pattaramalai S, et al. 1996. Exp. Cell. Res. 222:281.
  2. Skubitz AP, et al. 1996. Am. J. Pathol. 148:1445. (IHC)
  3. Skubitz AP, et al. 1998. FEBS Lett. 426:386.
  4. Calzada MJ, et al. 2004. Circ. Res. 94:462. (IP)
  5. Zhang X, et al. 2003. Surgery. 133:429. (WB)
Product Citations
  1. Voit RA, et al. 2023. Nat Immunol. 24:69. PubMed
  2. Leduc A, et al. 2022. Genome Biol. 23:261. PubMed
  3. Bao EL, et al. 2020. Nature. 769:586. PubMed
  4. Shlamkovich T, et al. 2020. BMC Biology. 16(1):92. PubMed
RRID
AB_10641282 (BioLegend Cat. No. 343808)

Antigen Details

Structure
150 kD type I transmembrane glycoprotein, integrin family, associate with integrin β1(CD29)
Distribution
Endothelial cells, epithelial cells, dermal fibroblast
Function
adhesion
Ligand/Receptor
Kalinin, collagen, laminin-1, laminin-5 and entactin, fibronectin
Cell Type
Endothelial cells, Epithelial cells, Fibroblasts
Biology Area
Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Zola H, et al. 2007. Leukocyte and stromal Cell Molecules:the CD Markers. John Wiley & Sons Inc.

Gene ID
3675 View all products for this Gene ID
UniProt
View information about CD49c on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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