Loading...

APC anti-human MPO

Pricing & Availability
Clone
MPO421-8B2 (See other available formats)
Regulatory Status
RUO
Other Names
Myeloperoxidase
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
MPO421-8B2_APC_MPO_Antibody_032525
Human peripheral blood leukocytes were fixed and permeabilized, then intracellularly stained with anti-human MPO (MPO421-8B2) APC (left) or mouse IgG1, κ APC isotype control (right).
  • MPO421-8B2_APC_MPO_Antibody_032525
    Human peripheral blood leukocytes were fixed and permeabilized, then intracellularly stained with anti-human MPO (MPO421-8B2) APC (left) or mouse IgG1, κ APC isotype control (right).
See APC spectral data
Cat # Size Price Quantity Check Availability Save
347209 25 tests £139
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Myeloperoxidase (MPO) is a heterotetrameric protein consisting of two 60 kD heavy units and two 12 kD light units. A lysosomal enzyme, MPO is able to catalyze the production of hypochlorous acid, a potent microbicidal agent, from hydrogen peroxide and chloride anion during the neutrophil respiratory burst. MPO is a major enzyme involved in the inflammatory responses of polymorphonuclear leucocytes. MPO is localized to the azurophilic granules of mature granulocytes and monocytes and is also expressed in some acute myeloid leukemia cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Additional Product Notes

MPO Staining Procedure

  1. Prepare cells of interest and perform cell surface antigen staining as described in BioLegend’s Cell Surface Immunofluorescence Staining Protocol or Cell Surface Immunofluorescence Staining Protocol for Whole Blood.
  2. Add 50 μL of cell suspension to each tube.
  3. Add 100 μL of FluoroFix™ Buffer (Cat. No. 422101) to each tube and vortex immediately for at least 3 seconds. Assure no sample remains on the side of each tube.
  4. Incubate at room temperature in the dark for 15 minutes.
  5. During fixation, prepare antibody dilutions (if needed) in 1X Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002) (typically in final volume of 50 μL)
  6. Vortex fixed samples briefly, then add 600 μL of 1X Intracellular Staining Permeabilization Wash Buffer to each tube and vortex immediately for at least 3 seconds.
  7. Add appropriate amount of antibody diluted in 1X Intracellular Staining Permeabilization Wash Buffer, vortex briefly, and incubate for 30 minutes in the dark at room temperature.
  8. Add 1 mL of FluoroFix™ Buffer and vortex immediately for at least 10 seconds.
  9. Centrifuge at 300 x g for 5 minutes, decant to discard supernatant
  10. Resuspend cells of each tube in 350 μL of FluoroFix™ buffer
  11. Interrogate by flow cytometry using proper machine settings. Acquire samples within 2 hours. 
RRID
AB_3683370 (BioLegend Cat. No. 347209)

Antigen Details

Structure
MPO is a tetrameric protein, each tetramer contains a two 60 kD heavy units and two 12 kD light units. It is a lysosomal hemoprotein stored in azurophilic granules.
Distribution

Neutrophils, monocytes, and some acute myeloid leukemia cells

Cell Type
Leukemia, Monocytes, Neutrophils
Biology Area
Immunology
Molecular Family
Enzymes and Regulators
Antigen References

1. Goedken M, et al. 2007. J. Biol. Chem. 282:27994
2. Nauseef WM, et al. 1988. Euro. J. Haemat. 40:97
3. Kelebanoff SJ, et al.1999. Proc. Assoc. Am. Phys. 111:383

Gene ID
4353 View all products for this Gene ID
UniProt
View information about MPO on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 02/13/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account