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APC anti-mouse CD140a Antibody

Pricing & Availability
Clone
APA5 (See other available formats)
Regulatory Status
RUO
Other Names
PDGF receptor-α, PDGFR-α
Isotype
Rat IgG2a, κ
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Product Citations
publications
APA5_APC_030510
Mouse fibroblast NIH/3T3 cells stained with APA5 APC
  • APA5_APC_030510
    Mouse fibroblast NIH/3T3 cells stained with APA5 APC
Compare all formats See APC spectral data
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135907 25 µg £62
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135908 100 µg £183
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Description

Platelet-derived growth factor receptor-α (PDGFR-α), CD140a, is one of two receptors for platelet-derived growth factors (PDGFs) and binds to all isoforms of PDGFs: PDGF-AA, PDGF-AB, and PDGF-BB. PDGFRa is a receptor tyrosine kinase that forms homodimers or heterodimers on the surface upon ligand binding and phosphorylates substrates. PDGFRs consist of either homodimers of α/α, β/β, or heterodimers of α/β. PDGF receptors, α and β, are single glycoproteins with intracellular tyrosine kinase domain. Their ligand, PDGF, is a mitogen for connective tissue and glial cells. CD140a is expressed on embryonic tissues and mesenchymal-derived cells of adult mice. PDGF plays a role in wound healing and acts as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes, and neutrophils.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse PDGFR-α-hIgG1 recombinant fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported (for relevant formats) applications include: Western Blot, blocking function2, and immunohistochemical staining of paraffin and frozen sections. The LEAF™ purified antibody is recommended for functional assays.

Application References

(PubMed link indicates BioLegend citation)
  1. Takakura N, et al. 1996. J. Invest. Dermatol. 107:770.
  2. Liao C, et al. 2010. J. Clin. Invest. 120:242. (Block)
  3. Chen H, et al. 2015. ASN Neuro 8:7. PubMed.
  4. Miyawaki T, et al. 2004. J Neurosci. 24(37): 8124-34. (IHC-F)
Product Citations
  1. Wei Y, et al. 2021. Elife. 10: . PubMed
  2. Vasanthakumar A, et al. 2020. Nature. 579:581. PubMed
  3. Hattori Y, et al. 2022. J Neurosci. 42:362. PubMed
  4. Agarwalla P, et al. 2022. Nat Biotechnol. 40:1250. PubMed
  5. Sastourné-Arrey Q, et al. 2023. Nat Commun. 14:80. PubMed
  6. Jadhav U et al. 2017. Cell stem cell. 21(1):65-77 . PubMed
  7. Korsunsky I, et al. 2022. Med. 3:481. PubMed
  8. Akasaka H, et al. 2023. JCI Insight. 8:. PubMed
  9. Corna G, et al. 2016. J Immunol. 197: 1914 - 1925. PubMed
  10. Wietecha MS, et al. 2020. Nat Commun. 2.266666667. PubMed
  11. Chen J, et al. 2021. Sci Adv. 7:. PubMed
  12. Wang W, et al. 2019. Cell Metab. 30:174. PubMed
  13. Nawaz A, et al. 2022. Nat Commun. 13:7058. PubMed
  14. Vazquez-Armendariz AI, et al. 2021. STAR Protocols. 2(2):100594. PubMed
  15. Jard T, et al. 2020. Cell Stem Cell. 27(4):646-662.e7. PubMed
  16. Zhang X, et al. 2020. Cell Reports. 28(3):792-803.e4.. PubMed
  17. Chen YT, et al. 2021. Nat Commun. 12:6845. PubMed
  18. Paris AJ, et al. 2020. Nat Cell Biol. 1.747916667. PubMed
  19. Gui J, et al. 2020. Nat Cancer. 1:603. PubMed
  20. Kinsella S, et al. 2021. Cell Rep. 37:109789. PubMed
  21. Ghelman J, et al. 2021. Glia. 69(11):2699-2716. PubMed
  22. Wang B,et al. 2017. Cell Discov.. 10.1038/celldisc.2017.36. PubMed
  23. Reddy A, et al. 2020. Cell. 183:62. PubMed
  24. Bahar Halpern K, et al. 2020. Nat Commun. 11:1936. PubMed
  25. Progatzky F, et al. 2021. Nature. 599:125. PubMed
  26. Ko T, et al. 2022. Nat Commun. 13:3275. PubMed
  27. Taguchi A, et al. 2017. Cell Stem Cell. 21:730. PubMed
  28. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
  29. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  30. Shook BA, et al. 2020. Cell Stem Cell. 26(6):880-895. PubMed
  31. Rebekka K Schneider et al. 2017. Cell stem cell. 20(6):785-800 . PubMed
  32. Hepler C et al. 2018. eLife. 7 pii: e39636. PubMed
  33. Hiebert P et al. 2018. Developmental cell. 46(2):145-161 . PubMed
  34. Zhao T et al. 2018. Cell stem cell. 23(1):31-45 . PubMed
  35. Murakami Y, et al. 2019. Sci Rep. 1.188888889. PubMed
  36. Shi X, et al. 2022. Clin Transl Med. 12:e711. PubMed
  37. Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed
  38. Jing Y, et al. 2020. Cancer Gene Ther. 27:910. PubMed
  39. Hogarth MW, et al. 2019. Nat Commun. 10:2430. PubMed
  40. Avraham S, et al. 2019. Oncogene. 38:3812. PubMed
  41. Wang QA et al. 2018. Cell metabolism. 28(2):282-288 . PubMed
  42. Hu Y, et al. 2021. EMBO Rep. 22:e52481. PubMed
  43. Nowlan B, et al. 2019. Haematologica. 105:71. PubMed
  44. Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed
  45. Moiseenko A, et al. 2020. Cell Rep. 33:108549. PubMed
  46. Lu Z, et al. 2021. J Clin Invest. 131:. PubMed
  47. Wang W, et al. 2014. Proc Natl Acad Sci U S A. 111:14466. PubMed
  48. Kurowska-Stolarska M, et al. 2016. J Allergy Clin Immunol. S0091-6749(16)31132-0. PubMed
  49. Grainger N, et al. 2020. J Physiol. 598:3283. PubMed
  50. Cangkrama M, et al. 2020. EMBO Mol Med. 12:e11466. PubMed
  51. Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed
  52. Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed
  53. Chandrakanthan V, et al. 2016. Proc Natl Acad Sci U S A. 113: 2306-2315. PubMed
  54. Kastenschmidt JM, et al. 2021. Cell Rep. 35:108997. PubMed
  55. Pinho AV, et al. 2018. Nat Commun. 9:5083. PubMed
  56. Wong PP, et al. 2020. Cell. 181(6):1346-1363.e21. PubMed
RRID
AB_2043969 (BioLegend Cat. No. 135907)
AB_2043969 (BioLegend Cat. No. 135908)

Antigen Details

Structure
Alpha chain of the platelet-derived growth factor receptor, a receptor tyrosine kinase that forms homo- or hetero-dimers on the surface after ligand binding.
Distribution

Expressed on embryonic tissues and mesenchymal-derived cells of adult mouse.

Function
Play a role in wound healing and act as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes and neutrophils.
Ligand/Receptor
PDGFs
Cell Type
Embryonic Stem Cells, Mesenchymal cells, Mesenchymal Stem Cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Mukouyama YS, et al. 2006. Proc Natl Acad Sci USA. 103(5):1551
2. Miyawaki T, et al. 2004. J Neurosci. 24(37):8124
3. Takakura N, et al. 1997. J Histochem Cytochem. 45(6):883

Gene ID
18595 View all products for this Gene ID
UniProt
View information about CD140a on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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