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Purified anti-mouse CD140a Antibody

Pricing & Availability
Clone
APA5 (See other available formats)
Regulatory Status
RUO
Other Names
PDGF receptor-α, PDGFR-α
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A_APA5_PE_112409
Mouse fibroblast cell line NIH/3T3 stained with APA5 PE
  • A_APA5_PE_112409
    Mouse fibroblast cell line NIH/3T3 stained with APA5 PE
  • B_APA5_PE_112409
    C57BL/6 splenocytes stained with APA5 PE
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135901 25 µg £37
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135902 100 µg £74
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Description

Platelet-derived growth factor receptor-α (PDGFR-α), CD140a, is one of two receptors for platelet-derived growth factors (PDGFs) and binds to all isoforms of PDGFs: PDGF-AA, PDGF-AB, and PDGF-BB. PDGFRa is a receptor tyrosine kinase that forms homodimers or heterodimers on the surface upon ligand binding and phosphorylates substrates. PDGFRs consist of either homodimers of α/α, β/β, or heterodimers of α/β. PDGF receptors, α and β, are single glycoproteins with intracellular tyrosine kinase domain. Their ligand, PDGF, is a mitogen for connective tissue and glial cells. CD140a is expressed on embryonic tissues and mesenchymal-derived cells of adult mice. PDGF plays a role in wound healing and acts as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes, and neutrophils.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse PDGFR-α-hIgG1 recombinant fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
WB, IHC-P, IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported (for relevant formats) applications include: Western Blot, blocking function2, and immunohistochemical staining of paraffin and frozen sections. The LEAF™ purified antibody is recommended for functional assays.

Application References

(PubMed link indicates BioLegend citation)
  1. Takakura N, et al. 1996. J. Invest. Dermatol. 107:770.
  2. Liao C, et al. 2010. J. Clin. Invest. 120:242. (Block)
  3. Chen H, et al. 2015. ASN Neuro 8:7. PubMed.
  4. Miyawaki T, et al. 2004. J Neurosci. 24(37): 8124-34. (IHC-F)
Product Citations
  1. Kim J, et al. 2016. PLoS One. 11: 0158067. PubMed
  2. Dittmer M, et al. 2018. Mol Brain. 11:25. PubMed
  3. Engelbrecht E, et al. 2022. Microcirculation. 29:e12752. PubMed
  4. Weng L, et al. 2016. Sci Rep. 6:38632. PubMed
  5. Albrecht S, et al. 2016. PLoS One. 11:e0163841. PubMed
  6. Paulo E, et al. 2018. Sci Rep. 8:11001. PubMed
  7. Jarjour AA, et al. 2020. Glia. 68(9):1840-1858. PubMed
  8. Gibson EM, et al. 2019. Cell. 176:43:00. PubMed
  9. Ghelman J, et al. 2021. Glia. 69(11):2699-2716. PubMed
  10. Chen H, et al. 2015. ASN Neuro. 8:7. PubMed
  11. Liu M, et al. 2018. Diabetologia. 61:2333. PubMed
  12. Shin J, et al. 2018. Diabetes. 67:1068. PubMed
  13. Yoshioka K, et al. 2020. Front Cell Dev Biol. 0.884027778. PubMed
  14. Yin W, et al. 2022. Development. 149:. PubMed
  15. Iida Y, et al. 2020. J Immunother Cancer. 8:. PubMed
  16. Maller O, et al. 2020. Nat Mater. 20:548. PubMed
RRID
AB_1953328 (BioLegend Cat. No. 135901)
AB_1953328 (BioLegend Cat. No. 135902)

Antigen Details

Structure
Alpha chain of the platelet-derived growth factor receptor, a receptor tyrosine kinase that forms homo- or hetero-dimers on the surface after ligand binding.
Distribution

Expressed on embryonic tissues and mesenchymal-derived cells of adult mouse.

Function
Play a role in wound healing and act as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes and neutrophils.
Ligand/Receptor
PDGFs
Cell Type
Embryonic Stem Cells, Mesenchymal cells, Mesenchymal Stem Cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Mukouyama YS, et al. 2006. Proc Natl Acad Sci USA. 103(5):1551
2. Miyawaki T, et al. 2004. J Neurosci. 24(37):8124
3. Takakura N, et al. 1997. J Histochem Cytochem. 45(6):883

Gene ID
18595 View all products for this Gene ID
UniProt
View information about CD140a on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 03/25/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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