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APC anti-mouse CD25 Antibody

Pricing & Availability
Clone
3C7 (See other available formats)
Regulatory Status
RUO
Other Names
IL-2Rα, Ly-43, p55, Tac
Isotype
Rat IgG2b, κ
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Product Citations
publications
3C7_APC_CD25_Antibody_101518
ConA/IL-2 stimulated (3 days) C57BL/6 splenocytes were stained with CD25 (clone 3C7) APC (filled histogram) or rat IgG2b, κ APC isotype control (open histogram).
  • 3C7_APC_CD25_Antibody_101518
    ConA/IL-2 stimulated (3 days) C57BL/6 splenocytes were stained with CD25 (clone 3C7) APC (filled histogram) or rat IgG2b, κ APC isotype control (open histogram).
Compare all formats See APC spectral data
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101909 25 µg £70
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101910 100 µg £204
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Description

CD25 is a 55 kD glycoprotein, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132(common γ chain), CD25 forms the high affinity signaling IL-2 receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-dependent BALB/c mouse helper T-cell clone HT-2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: in vitro blocking of IL-2 binding to low- and high-affinity receptors1,2, and immunohistochemical staining of acetone-fixed frozen sections. 3C7 antibody recognizes different epitope of PC61 antibody (Cat. No. 102002). The Ultra-LEAF™Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 101925 and 101926).

Application References

(PubMed link indicates BioLegend citation)
  1. Ortega RG, et al. 1984. J. Immunol. 133:1970. (Block)
  2. Moreau JL, et al. 1987. Eur. J. Immunol. 17:929. (Block)
Product Citations
  1. Sohn HS, et al. 2022. Sci Adv. 8:eabo5284. PubMed
  2. Wang Y, et al. 2023. iScience. 26:106630. PubMed
  3. Yao RQ, et al. 2023. Mil Med Res. 10:27. PubMed
  4. Shen Z, et al. 2022. EBioMedicine. 85:104285. PubMed
  5. Zhu M, et al. 2022. Front Immunol. 13:1019248. PubMed
  6. Laura C Burzynski et al. 2019. Immunity. 50(4):1033-1042 . PubMed
  7. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
  8. Li X, et al. 2019. Mol Med Rep. 19:4195. PubMed
  9. Rosalia R, et al. 2013. Immunobiology. 218:851. PubMed
  10. Andreas N, et al. 2021. Arthritis Res Ther. 23:222. PubMed
  11. Periasamy S, et al. 2017. Nat Commun. 8:15564. PubMed
  12. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  13. Gorczynski RM, et al. 2017. Immunology. 150:418. PubMed
  14. Ren HZ, et al. 2022. J Clin Transl Hepatol. 10:1138. PubMed
  15. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  16. Fujiwara H, et al. 2021. Nat Immunol. 22:1440. PubMed
  17. Shi R, et al. 2022. Theranostics. 12:875. PubMed
  18. Akazawa S, et al. 2021. Diabetologia. 64:878. PubMed
  19. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
  20. Sharma M, et al. 2020. Circ Res. 127:335. PubMed
  21. Rashid MH, et al. 2021. Oncol Rep. 45:1171. PubMed
  22. Mushaben E, et al. 2011. J Immunol. 187:5756. PubMed
  23. Pham D, et al. 2019. Cell Rep. 29:1203. PubMed
  24. Ali S, et al. 2021. PLoS One. 16:e0246646. PubMed
  25. Yang Z, et al. 2021. Nat Commun. 12:4852. PubMed
  26. Li J, et al. 2022. Nat Commun. 13:4032. PubMed
  27. Zeng Q, et al. 2022. iScience. 25:105151. PubMed
  28. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  29. Takaku S, et al. 2022. Evid Based Complement Alternat Med. 2022:6122955. PubMed
  30. Zhang S, et al. 2022. Nat Commun. 13:4744. PubMed
  31. Wang Y, et al. 2021. Nat Commun. 12:4964. PubMed
  32. He S, et al. 2021. Front Pharmacol. 12:743837. PubMed
  33. Kiuchi M, et al. 2021. J Exp Med. 218:. PubMed
  34. Di Lorenzo A, et al. 2022. Oncoimmunology. 11:2086752. PubMed
  35. Chen C, et al. 2020. Cell Rep. 2136:30. PubMed
  36. Baglaenko Y, et al. 2016. PLoS One. 11: 0150515. PubMed
  37. MaruYama T, et al. 2015. J Leukoc Biol. 98: 385-393. PubMed
  38. Wang J, et al. 2019. Aging (Albany NY). 11:3463. PubMed
  39. Blaszczak AM, et al. 2020. Immunometabolism. 2:00. PubMed
  40. Singh KS, et al. 2020. Nat Commun. 0.786805556. PubMed
  41. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  42. Satoh–Takayama N, et al. 2020. Immunity. 52(4):635-649. PubMed
  43. Heckler M, et al. 2021. Cancer Discov. Online ahead of prin. PubMed
  44. Sharma M, et al. 2019. Immunometabolism. 1. PubMed
  45. Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
  46. Le TM et al. 2018. Journal of neurochemistry. 145(2):139-153 . PubMed
  47. Lee S, et al. 2021. Autophagy. 18:1062. PubMed
  48. Li X, et al. 2021. Front Cell Dev Biol. 9:647713. PubMed
  49. Scott G, et al. 2009. J Immunol. 182:7509. PubMed
  50. Tsai S, et al. 2018. Cell Metab. 28:922. PubMed
RRID
AB_2280288 (BioLegend Cat. No. 101909)
AB_2280288 (BioLegend Cat. No. 101910)

Antigen Details

Structure
Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
Distribution

Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells

Function
IL-2 receptor, signal transduction
Ligand/Receptor
IL-2
Cell Type
B cells, T cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann TA. 1991. J. Biol. Chem. 266:2681.
3. Read S, et al. 2000. J. Exp. Med. 192:295.
4. Lowenthal JW, et al. 1985. J. Immunol. 135:3988.

Gene ID
16184 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org
Go To Top Version: 2    Revision Date: 10/15/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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