- Clone
- PC61 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- IL-2Rα, Ly-43, p55, Tac
- Isotype
- Rat IgG1, λ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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CD25 is a 55 kD glycoprotein also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- IL-2-dependent cytolytic mouse T-cell clone B6.1
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
IHC-F, IP, Block, Depletion - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, in vitro blocking of IL-2 binding to low- and high-affinity receptors1-4, growth inhibition of IL-2-dependent T-cell lines1-4, in vivo depletion of CD25+CD4+ Treg cells5-8,10, and immunohistochemical staining of acetone-fixed frozen sections2. PC61 antibody recognizes a different epitope than 3C7 antibody (Cat. No. 101902). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 102040) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered.
-
Application References
(PubMed link indicates BioLegend citation) -
- Lowenthal JW, et al. 1985. Nature 315:669. (IP, Block)
- Ceredig R, et al. 1985. Nature 314:98. (IP, IHC, Block)
- Lowenthal JW, et al. 1985. J. Immunol. 135:3988. (Block)
- Moreau JL, et al. 1987. Eur. J. Immunol. 17:929. (Block)
- Takahashi T, et al. 2000. J. Exp. Med. 192:303. (Deplete)
- Onizuka S, et al. 1999. Cancer Res. 59:3128. (Deplete)
- Lei TC, et al. 2005. Blood 105:4865. (Deplete)
- Pasare C, et al. 2004. Immunity 21:733. (Deplete)
- León-Ponte M, et al. 2007. Blood 109:3139.
- Cao OW, et al. 2007. Blood doi:10.1182/blood-2007-02-073304. (Deplete)
- Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719.
- Liu F, et al. 2011. Arch Toxicol. 85:1383. PubMed
- Anguela XM, et al. 2013. Diabetes. 62:551. PubMed
- Product Citations
-
- RRID
-
AB_2813924 (BioLegend Cat. No. 102057)
AB_2813924 (BioLegend Cat. No. 102040)
AB_2813924 (BioLegend Cat. No. 102058)
AB_2813924 (BioLegend Cat. No. 102059)
AB_2813924 (BioLegend Cat. No. 102060)
AB_2813924 (BioLegend Cat. No. 102061)
Antigen Details
- Structure
- Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
- Distribution
-
Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells
- Function
- IL-2 receptor
- Ligand/Receptor
- IL-2
- Cell Type
- B cells, T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
- Taniguchi T, et al. 1993. Cell 73:5-8.
- Waldmann TA. 1991. J Biol Chem. 266:2681-4.
- Read S, et al. 2000. J Exp Med. 192:295-302.
- Lowenthal JW, et al. 1985. J Immunol. 135:3988-94.
- Gene ID
- 16184 View all products for this Gene ID
- UniProt
- View information about CD25 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD25 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-mouse CD25
Con A-stimulated (3 days)splenocytes stained with PC61 APC -
Biotin anti-mouse CD25
Con A-stimulated C57BL/6 splenocytes (3 days) stained with ... -
FITC anti-mouse CD25
Con-A Stimulated (3 days) BALB/c mouse splenocytes stained w... -
PE anti-mouse CD25
Con A-stimulated (3 days) BALB/c mouse splenocytes stained w... -
PE/Cyanine5 anti-mouse CD25
Con A-stimulated (day-3) C57BL/6 mouse splenocytes stained w... -
Purified anti-mouse CD25
C57BL/6 mouse splenocytes stained with purified PC61, follow... Fresh, frozen mouse spleen was stained with purified CD25 cl... -
PE/Cyanine7 anti-mouse CD25
Con A-stimulated (2 days) BALB/c mouse splenocytes stained w... -
Alexa Fluor® 488 anti-mouse CD25
Con A-stimulated (3 days) BALB/c mouse splenocytes stained w... -
Alexa Fluor® 647 anti-mouse CD25
Con A-stimulated (3 days) BALB/c mouse splenocytes stained w... -
Pacific Blue™ anti-mouse CD25
Con A-stimulated (3 days) C57BL/6 mouse splenocytes stained ... -
Alexa Fluor® 700 anti-mouse CD25
Con A-stimulated (day-3) C57BL/6 mouse splenocytes stained w... -
APC/Cyanine7 anti-mouse CD25
Con A-stimulated (day-3) C57BL/6 mouse splenocytes stained w... -
PerCP/Cyanine5.5 anti-mouse CD25
Con A-stimulated C57BL/7 splenocytes (Day 3) stained with PC... -
PerCP anti-mouse CD25
Con A-stimulated C57BL/7 splenocytes (Day 3) stained with P... -
Brilliant Violet 421™ anti-mouse CD25
C57BL/6 mouse splenocytes were stained with CD4 FITC and CD2... -
Brilliant Violet 605™ anti-mouse CD25
C57BL/6 mouse splenocytes were stained with CD4 PE and CD25 ... -
Brilliant Violet 650™ anti-mouse CD25
C57BL/6 mouse splenocytes were stained with CD4 FITC and CD2... -
Ultra-LEAF™ Purified anti-mouse CD25
Con A-stimulated (3 days) BALB/c mouse splenocytes stained w... -
Brilliant Violet 510™ anti-mouse CD25
Con A-stimulated (3 days) Balb/c splenocytes were stained wi... -
PE/Dazzle™ 594 anti-mouse CD25
Con A-stimulated (3 days) C57BL/6 mouse splenocytes were sta... -
Brilliant Violet 711™ anti-mouse CD25
Con A-stimulated (3 days) C57BL/6 mouse splenocytes were sta... -
Brilliant Violet 785™ anti-mouse CD25
Con A-stimulated (3 days) C57BL/6 mouse splenocytes were sta... -
Alexa Fluor® 594 anti-mouse CD25
C57BL/6 mice were intraperitoneally injected with 100 µg LEA... -
APC/Fire™ 750 anti-mouse CD25
C57BL/6 mouse splenocytes were stained with CD4 PE and CD25 ... -
TotalSeq™-A0097 anti-mouse CD25
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KIRAVIA Blue 520™ anti-mouse CD25
Con A-stimulated (3 days) C57BL/6 mouse splenocytes were sta... C57BL/6 mouse splenocytes were stained with CD4 APC and CD25... -
TotalSeq™-B0097 anti-mouse CD25
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TotalSeq™-C0097 anti-mouse CD25
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Spark NIR™ 685 anti-mouse CD25 Antibody
Con A-stimulated (day-3) C57BL/6 mouse splenocytes were stai... -
PE/Fire™ 640 anti-mouse CD25
Con A-stimulated (3 days) C57BL/6 mouse splenocytes were sta... C57BL/6 mouse splenocytes were stained with anti-mouse CD4 F... -
Spark YG™ 581 anti-mouse CD25
C57BL/6 mouse splenocytes were stained with anti-mouse CD4 P... -
APC/Fire™ 810 anti-mouse CD25
Con A-stimulated (3 days) C57BL/6 mouse splenocytes were sta... -
Brilliant Violet 750™ anti-mouse CD25
Mouse (C57BL/6) splenocytes were stained with anti-mouse CD4... -
PerCP/Fire™ 780 anti-mouse CD25
C57BL/6 mouse splenocytes were stained with anti-mouse CD4 A... -
PE/Fire™ 700 anti-mouse CD25
C57BL/6 mouse splenocytes were stained with anti-mouse CD4 (... -
Spark PLUS UV395™ anti-mouse CD25
C57BL/6 mouse splenocytes were stained with anti-mouse CD4 (... -
Spark Blue™ 574 anti-mouse CD25 (Flexi-Fluor™)
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