APC anti-mouse IgG1 Antibody

Pricing & Availability
Clone
RMG1-1 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin G1
Isotype
Rat IgG
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Product Citations
publications
RMG1-1_APC_111710
Human peripheral blood lymphocytes were stained with purified mouse IgG1 anti-human CD3 (clone UCHT1, filled histogram) or mouse IgG1, k isotype control (open histogram), followed by anti-mouse IgG1 (clone RMG1-1) APC.
  • RMG1-1_APC_111710
    Human peripheral blood lymphocytes were stained with purified mouse IgG1 anti-human CD3 (clone UCHT1, filled histogram) or mouse IgG1, k isotype control (open histogram), followed by anti-mouse IgG1 (clone RMG1-1) APC.
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Cat # Size Price Quantity Check Availability Save
406609 50 µg £78
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406610 200 µg £223
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Description

The RMG1-1 monoclonal antibody reacts with immunoglobulin G1 (IgG1) in all tested mouse haplotype (Igh-a and b). It does not react with other isotypes. The RMG1-1 monoclonal antibody may be used as a primary or secondary reagent for ELISA or immunofluorescent analysis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Rucci F, et al. 2010. P. Natl. Acad. Sci. USA 107:3024. (FC) PubMed
  2. Zhang JG, et al. 2011. Int. Immunopharmacol. 11:1685. PubMed
Product Citations
  1. Minott JA, et al. 2022. Front Immunol. 13:1038340. PubMed
  2. Lombard-Vadnais F, et al. 2023. iScience. 26:105852. PubMed
  3. Ivasko SM, et al. 2023. Front Immunol. 13:1023206. PubMed
  4. Rydyznski CE et al. 2018. Cell reports. 24(13):3367-3373 . PubMed
  5. Ehling RA, et al. 2022. Cell Rep. 38:110242. PubMed
  6. Bhattacharya Y 2014. J Exp Med. 211:841. PubMed
  7. Ishii M, et al. 2021. Pharmaceutics. 13:. PubMed
  8. Hung PJ, et al. 2018. Mol Cell. 71:332. PubMed
  9. Manresa MC, et al. 2020. Gastroenterology. 159:1778. PubMed
  10. Collin R, et al. 2014. J Immunol. 193:3503. PubMed
  11. Günal-Sadık G, et al. 2014. PLoS One. 9:111748. PubMed
  12. Duan L, et al. 2021. Immunity. 54:2256. PubMed
  13. Hare DN, et al. 2021. STAR Protoc. 2:100436. PubMed
  14. De Veirman K, et al. 2021. Oncoimmunology. 10:2000699. PubMed
  15. Gradiz R, et al. 2016. Sci Rep. 6:21648. PubMed
  16. Vergadi E, et al. 2014. J Immunol. 192:394. PubMed
  17. Kim S, et al. 2021. Animals (Basel). 11:. PubMed
  18. Clancy–Thompson E, et al. 2019. EMBO J. 38:e101260. PubMed
  19. Sanchez HN, et al. 2020. Nat Commun. 0.5. PubMed
  20. Deen D, et al. 2021. Blood Adv. 5:3002. PubMed
  21. Habets T, et al. 2016. PLoS One. 11: 0159515. PubMed
  22. Shimba A et al. 2018. Immunity. 48(2):286-298 . PubMed
  23. Stokes K, et al. 2013. J Virol. 87:10070. PubMed
  24. Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
  25. Cronin J, et al. 2016. Mucosal Immunol. 10.1038/mi.2015.131. PubMed
  26. Roberts JT, et al. 2020. Mol Cell Proteomics. 19:362. PubMed
  27. Jang A, et al. 2021. Front Immunol. 12:701066. PubMed
  28. Aguilar–Briseñ o JA, et al. 2020. Nat Commun. 2.664583333. PubMed
RRID
AB_10679040 (BioLegend Cat. No. 406609)
AB_10679040 (BioLegend Cat. No. 406610)

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Go To Top Version: 2    Revision Date: 01/29/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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