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APC anti-mouse LPAM-1 (Integrin α4β7) Antibody

Pricing & Availability
Clone
DATK32 (See other available formats)
Regulatory Status
RUO
Other Names
α4β7 Integrin, CD49d/β7, LPAM-1, ITGA4, ITGB7
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
DATK32_APC_082610
C57BL/6 splenocytes stained with DATK32 APC
  • DATK32_APC_082610
    C57BL/6 splenocytes stained with DATK32 APC
  • DATK32_APC_1_101310
    C57BL/6 mouse bone marrow cells were surface stained with B220 PE and LPAM-1 APC (clone DATK32) (top) or rat IgG2a, κ APC isotype control (bottom).
  • DATK32_APC_2_101310
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120607 25 µg £68
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120608 100 µg £204
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Description

DATK32 antibody is specific for a combinatorial determinate of integrin α4β7complex. Integrin α4β7 is composed of a 150 kD (α4 or CD49d) and a 130 kD (β7) heterodimer, also known as CD49d/β7 or LPAM-1. Belonging to the Ig superfamily, it is found on the majority of peripheral lymphocytes and subsets of thymocytes and bone marrow cells (including mast cell progenitors). Integrin α4β7 binds its ligands, VCAM-1 (CD106), MAdCAM-1 and fibronectin, and plays an important role in lymphocytes adhesion and the direction of migration of blood lymphocytes to the intestine and associated lymphoid tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
TK1 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of cell adhesion1-4, in vitro induction of TK1 cell aggregation1, immunoprecipitation4, and immunohistochemical staining of frozen sections5. It has been reported that the DATK32 mAb is able to block a4ß7 mediated lymphocyte adhesion to VCAM-1, MAdCAM-1, and fibronectin in vitro and in vivo. 

Application References

(PubMed link indicates BioLegend citation)
  1. Andrew DP, et al. 1994. J. Immunol. 153:3847. (Block)
  2. Berlin C, et al. 1993. Cell 74:185. (Block)
  3. Berlin-Rufenach C, et al. 1999. J. Exp. Med. 189:1467. (Block)
  4. Rivera-Nieves J, et al. 2005. J. Immunol. 174:2343. (Block, IP)
  5. Bogetto L, et al. 2000. Blood 95:2397. (IHC)
Product Citations
  1. Behr FM, et al. 2021. Eur J Immunol. 51:151. PubMed
  2. Kawano H, et al. 2016. Int Immunol. 28: 489 - 501. PubMed
  3. Tian Y, et al. 2016. J Immunol. 196: 2153 - 2166. PubMed
  4. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  5. Knolle MD et al. 2018. Frontiers in immunology. 1.925 . PubMed
  6. Kishore M et al. 2017. Immunity. 47(5):875-889 . PubMed
  7. Calcinotto A, et al. 2018. Nat Commun. 9:4832. PubMed
  8. Ghaedi M, et al. 2020. The Journal of Experimental Medicine. 217(3):jem. PubMed
  9. Kitamoto S, et al. 2020. Cell. 182(2):447-462.e14. PubMed
RRID
AB_10719833 (BioLegend Cat. No. 120607)
AB_10719833 (BioLegend Cat. No. 120608)

Antigen Details

Structure
Integrin family, Ig superfamily, 150 kD/130 kD heterodimer
Distribution

Majority of peripheral lymphocytes, subsets of thymocytes and bone marrow cells

Function
Lymphocyte adhesion
Ligand/Receptor
VCAM-1 (CD106), MAdCAM-1, fibronectin
Biology Area
Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Andrew DP, et al. 1994. J. Immunol. 153:3847.
2. Berlin C, et al. 1994. Cell 74:185.
3. Gurish MF, et al. 2001 J. Exp. Med. 194:1243.
4. Hamann A, et al. 1994. J. Immunol. 152:3282.

Gene ID
311144 View all products for this Gene ID 25713 View all products for this Gene ID
UniProt
View information about LPAM-1 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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