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APC/Fire™ 750 anti-human CD11c Antibody

Pricing & Availability
Clone
3.9 (See other available formats)
Regulatory Status
RUO
Workshop
III NL707
Other Names
Integrin αX subunit, CR4, p150, ITGAX
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
3dot9_APCFIre750_CD11c_Antibody_1_072519.png
Human peripheral blood monocytes were stained with APC/Fire™ 750 anti-human CD11c (clone 3.9) (filled histogram) or mouse IgG1, κ APC/Fire™ 750 isotype control (open histogram)
  • 3dot9_APCFIre750_CD11c_Antibody_1_072519.png
    Human peripheral blood monocytes were stained with APC/Fire™ 750 anti-human CD11c (clone 3.9) (filled histogram) or mouse IgG1, κ APC/Fire™ 750 isotype control (open histogram)
  • 3dot9_APCFIre750_CD11c_Antibody_2_072519.png
    Human peripheral blood lymphocytes were stained with PE anti-human CD3 and APC/Fire™ 750 anti-human CD11c (clone 3.9) (left) or mouse IgG1, κ APC/Fire™ 750 isotype control (right).
Compare all formats See APC/Fire™ 750 spectral data
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301645 25 tests £121
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301646 100 tests £262
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Description

CD11c is a 145-150 kD type I transmembrane glycoprotein also known as integrin αX and CR4. CD11c non-covalently associates with integrin β2 (CD18) and is expressed on monocytes/macrophages, dendritic cells, granulocytes, NK cells, and subsets of T and B cells. CD11c has been reported to play a role in adhesion and CTL killing through its interactions with fibrinogen, CD54, and iC3b.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Chimpanzee, Squirrel Monkey
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone 3.9 preferentially binds the activated form of CD11c, is specific for the I domain of CD11c, and is able to partially block the binding of CD11c and ICAM-4. 3.9 binding is divalent cation dependent12. While analyzing blood, it is best to use heparin as the anti-coagulant and not EDTA. Since the ability of clone 3.9 to bind to its target is divalent cation dependent, the usage of EDTA as an anti-coagulant may be detrimental to staining due to its chelating properties.

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections4, and functional assays5,6. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 301616). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 301632) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W, et al. 1989. Leucocyte Typing IV Oxford University Press. New York.
  3. McMichael A, et al. Eds. 1987. Leucocyte Typing III Oxford University Press. New York.
  4. Vainer B, et al. 2000. Am. J. Surg. Pathol. 24:1115. (IHC)
  5. Ottonello L, et al. 1999. Blood 93:3505.
  6. Metelitsa LS, et al. 2002. Blood 99:4166.
  7. Sadhu C, et al. 2007. J. Leukoc. Biol. doi:10.1189/jlb.1106680. PubMed
  8. Ihanus E, et al. 2007. Blood 109:802-810.
  9. Gurer C, et al. 2008. Blood 112:1231. PubMed
  10. Asai A, et al. 2009. J. Lipid Res. 50:95. PubMed
  11. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  12. Sadhu C, et al. 2008. J. Immunoass. Immunoch. 29:42. (FC)
RRID
AB_2814121 (BioLegend Cat. No. 301645)
AB_2814121 (BioLegend Cat. No. 301646)

Antigen Details

Structure
Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 145-150 kD
Distribution

Myeloid, dendritic cells, NK cells, B cells and T cell subsets

Function
Adhesion, CTL killing
Ligand/Receptor
CD54, fibrinogen, iC3b, ICAM-1, ICAM-4
Cell Type
B cells, Dendritic cells, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Petty H. 1996. Immunol. Today 17:209.
2. Springer T. 1994. Cell 76:301.
3. Ihanus E, et al. 2007. Blood 109:802-810.

Gene ID
3687 View all products for this Gene ID
UniProt
View information about CD11c on UniProt.org
Go To Top Version: 1    Revision Date: 07/25/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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