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APC/Fire™ 750 anti-mouse H-2Kb bound to SIINFEKL Antibody

Pricing & Availability
Clone
25-D1.16 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class I molecule Kb bound to the peptide SIINFEKL (Kb-SIINFEKL) H-2Kb, OVA agonist peptide (OVAp), OVA-Kb, OVA257-264 (SIINFEKL)-H-2kb, OVA257-264-H-2kb
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
25-D116_APCFire750_H-2kb_Antibody_092718
C57BL/6 mouse splenocytes were pulsed with or without SIINFEKL for 2 hours, and then stained with anti-mouse SIINFEKL bound H-2Kb (clone 25-D1.16) APC/Fire™ 750 (purple filled histogram indicates the pulsed cells and cyan open histogram indicates non-pulsed cells) or mouse IgG1, κ APC/Fire™ 750 isotype control (black open histogram).
  • 25-D116_APCFire750_H-2kb_Antibody_092718
    C57BL/6 mouse splenocytes were pulsed with or without SIINFEKL for 2 hours, and then stained with anti-mouse SIINFEKL bound H-2Kb (clone 25-D1.16) APC/Fire™ 750 (purple filled histogram indicates the pulsed cells and cyan open histogram indicates non-pulsed cells) or mouse IgG1, κ APC/Fire™ 750 isotype control (black open histogram).
Compare all formats See APC/Fire™ 750 spectral data
Cat # Size Price Quantity Check Availability Save
141613 25 µg £89
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141614 100 µg £257
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Description

This antibody has been proven to be very useful in tracking the quantity and localization of these specific antigen-presenting cells (APC) in vivo.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SIINFEKL pulsed RMA-S cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.

Excitation Laser
Red Laser (633 nm)
Application Notes

The 25-D1.16 monoclonal antibody specifically reacts with ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I, but not with unbound H-2Kb or H-2Kb bound with an irrelevant peptide.  Additional reported applications (for relevant formats) include: immunofluorescence microscopy2,3, immunohistochemical staining of frozen tissue sections3 , and inhibition of T cell response to H-2Kb-SIINFEKL in vitro.

Application References

(PubMed link indicates BioLegend citation)
  1. Mareeva T, et al. 2010. J. Immunol. Methods 353:78. 
  2. Dolan BP, et al. 2010. J. Immunol. 184:1419. (IF)
  3. Porgador A, et al. 1997. Immunity 6:715. (IF, IHC)
  4. Herve J, et al. 2013. J. Immunol. 190:3163. PubMed.
Product Citations
  1. Tjärnhage E, et al. 2023. J Virol. e0169422:97. PubMed
RRID
AB_2750523 (BioLegend Cat. No. 141613)
AB_2750523 (BioLegend Cat. No. 141614)

Antigen Details

Structure
OVA peptide257-264 and H-2Kb complex
Distribution

SIINFEKL bond H-2Kb bearing cells

Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Mareeva T, et al. 2010. J. Immunol. Methods 353:78.
2. Mareeva T, et al. 2008. J. Biol. Chem. 283:29053.
3. Deng Y, et al. 1998. J. Immunol. 161:1677.

Gene ID
14972 View all products for this Gene ID
UniProt
View information about H-2Kb bound to SIINFEKL on UniProt.org
Go To Top Version: 1    Revision Date: 09/28/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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