- Clone
- 25-D1.16 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- MHC class I molecule Kb bound to the peptide SIINFEKL (Kb-SIINFEKL) H-2Kb, OVA agonist peptide (OVAp), OVA-Kb, OVA257-264 (SIINFEKL)-H-2kb, OVA257-264-H-2kb
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![25-D1.16_PE_050411 25-D1.16_PE_050411](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/25-D1.16_PE_050411.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were pulsed with (filled histogram) or without (cyan open histogram) SIINFEKL for 2 hours and then stained with 25-D1.16 PE or mouse IgG1, κ PE isotype control (broken line open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
141603 | 25 µg | £76 | ||||
141604 | 100 µg | £247 |
This antibody has been proven to be very useful in tracking the quantity and localization of these specific antigen-presenting cells (APC) in vivo.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- SIINFEKL pulsed RMA-S cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
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Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
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The 25-D1.16 monoclonal antibody specifically reacts with ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I, but not with unbound H-2Kb or H-2Kb bound with an irrelevant peptide. Additional reported applications (for relevant formats) include: immunofluorescence microscopy2,3, immunohistochemical staining of frozen tissue sections3 , and inhibition of T cell response to H-2Kb-SIINFEKL in vitro.
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Application References
(PubMed link indicates BioLegend citation) -
- Mareeva T, et al. 2010. J. Immunol. Methods 353:78.
- Dolan BP, et al. 2010. J. Immunol. 184:1419. (IF)
- Porgador A, et al. 1997. Immunity 6:715. (IF, IHC)
- Herve J, et al. 2013. J. Immunol. 190:3163. PubMed.
- Product Citations
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- RRID
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AB_10897938 (BioLegend Cat. No. 141603)
AB_10895905 (BioLegend Cat. No. 141604)
Antigen Details
- Structure
- OVA peptide257-264 and H-2Kb complex
- Distribution
-
SIINFEKL bond H-2Kb bearing cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
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1. Mareeva T, et al. 2010. J. Immunol. Methods 353:78.
2. Mareeva T, et al. 2008. J. Biol. Chem. 283:29053.
3. Deng Y, et al. 1998. J. Immunol. 161:1677. - Gene ID
- 14972 View all products for this Gene ID
- UniProt
- View information about H-2Kb bound to SIINFEKL on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All H-2Kb bound to SIINFEKL Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
PE anti-mouse H-2Kb bound to SIINFEKL | 25-D1.16 | FC |
APC anti-mouse H-2Kb bound to SIINFEKL | 25-D1.16 | FC,ICC |
PE/Cyanine7 anti-mouse H-2Kb bound to SIINFEKL | 25-D1.16 | FC |
APC/Fire™ 750 anti-mouse H-2Kb bound to SIINFEKL | 25-D1.16 | FC |
PE/Dazzle™ 594 anti-mouse H-2Kb bound to SIINFEKL | 25-D1.16 | FC |
TotalSeq™-C0231 anti-mouse H-2Kb bound to SIINFEKL | 25-D1.16 | PG |
TotalSeq™-B0231 anti-mouse H-2Kb bound to SIINFEKL | 25-D1.16 | PG |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-mouse H-2Kb bound to SIINFEKL
C57BL/6 mouse splenocytes were pulsed with (filled histogram... -
APC anti-mouse H-2Kb bound to SIINFEKL
C57BL/6 mouse splenocytes were pulsed with or without SIINFE... CHO cells expressing inducible single chain H-2Kb... -
PE/Cyanine7 anti-mouse H-2Kb bound to SIINFEKL
C57BL/6 mouse splenocytes were pulsed with or without SIINFE... -
APC/Fire™ 750 anti-mouse H-2Kb bound to SIINFEKL
C57BL/6 mouse splenocytes were pulsed with or without SIINFE... -
PE/Dazzle™ 594 anti-mouse H-2Kb bound to SIINFEKL
C57BL/6 mouse splenocytes were pulsed with or without SIINFE... -
TotalSeq™-C0231 anti-mouse H-2Kb bound to SIINFEKL
-
TotalSeq™-B0231 anti-mouse H-2Kb bound to SIINFEKL
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