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Avidin/Biotin Blocking System (Previously Covance catalog# SIG-31126)

Pricing & Availability
Regulatory Status
RUO
Other Names
Avidin/Biotin Blocking Kit
Previously
Covance Catalog# SIG-31126
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Product Citations
publications
Cat # Size Price Quantity Check Availability Save
927301 2 x 15 ml £71
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Technical Data Sheet (pdf)

Kit Contents

Kit Contents

• Avidin Solution, 15 mL
• Biotin Solution, 15 mL

Both reagents are provided ready-to-use and need no dilution

Product Details

Formulation
Blocking Kit. The Avidin and Biotin Solutions contain 0.09% sodium azide.

Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Storage & Handling
Store between 2°C and 8°C.
Application

IHC, WB
Recommended Usage

The optimal working dilution should be determined for each specific assay condition.

Blocking Procedure: 
For paraffin-embedded tissues: To be performed after the antigen retrieval and serum block but before primary antibody incubation.

1. Incubate the Avidin Solution for 10-15 minutes at room temperature.
2. Incubate with the Biotin Solution for 10-15 minutes at room temperature.

Application Notes

BioLegend's Avidin/Biotin Blocking System is intended for use in immunohistochemistry and immunoblotting procedures. The purpose is to decrease the signal caused by endogenous avidin, biotin, or biotin-binding proteins when immunostaining specimens with detection systems that rely on avidin-biotin or steptavidin-biotin chemistry. Nonspecific staining due to these factors commonly resembles a diffuse, weak, cytoplasmic pattern.

Product Citations
  1. Bednarski E, et al. 2022. MBio. 13:e0084022. PubMed
  2. Cacialli P, et al. 2022. EMBO J. 41:e108536. PubMed
  3. Deisher A, et al. 2022. STAR Protoc. 3:101663. PubMed
  4. Feldt J, et al. 2020. Osteoarthr Cartil Open. 2:100124. PubMed
  5. Olde Hanhof CJA, et al. 2023. Front Cell Dev Biol. 11:1086823. PubMed
  6. Mooney JP, et al. 2022. Wellcome Open Res. 7:134. PubMed
  7. Klarquist J, et al. 2021. Cell Rep. 36:109591. PubMed
  8. Ferrian S, et al. 2021. Cell Rep Med. 2:100419. PubMed
  9. Risom T, et al. 2022. Cell. 185:299. PubMed
  10. Scur M, et al. 2022. Nat Commun. 13:7272. PubMed
  11. Canedo EC, et al. 2021. JCI Insight. 6:. PubMed
  12. Elhanani O, et al. 2021. Methods Mol Biol. 2386:147. PubMed

Antigen Details

Gene ID
NA
Go To Top Version: 4    Revision Date: 02/08/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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