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Biotin anti-IP3R1 Antibody

Pricing & Availability
Clone
L24/18 (See other available formats)
Regulatory Status
RUO
Other Names
Inositol 1,4,5-trisphosphate receptor type 1, IP-3-R, IP3R 1, IP3 receptor, type 1 InsP3 receptor, inositol 1,4,5-triphosphate receptor 1, inositol 1,4,5-triphosphate receptor, type 1, type 1 inositol 1,4,5-trisphosphate receptor
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
L24slash18_Biotin_IP3R1_Antibody_1_012519
IHC staining of Biotin anti-IP3R1 antibody (Clone L24/18) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No.928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 594 Streptavidin (Cat. No. 405240) for one hour at room temperature. Nuclei were counterstained with Hoechst, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 10X objective. Scale Bar: 100 µm
  • L24slash18_Biotin_IP3R1_Antibody_1_012519
    IHC staining of Biotin anti-IP3R1 antibody (Clone L24/18) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No.928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 594 Streptavidin (Cat. No. 405240) for one hour at room temperature. Nuclei were counterstained with Hoechst, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 10X objective. Scale Bar: 100 µm
  • L24slash18_Biotin_IP3R1_Antibody_2_012519
    IHC staining of Biotin anti-IP3R1 antibody (Clone L24/18) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No.928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 594 Streptavidin (Cat. No. 405240) for one hour at room temperature. Nuclei were counterstained with Hoechst, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 10X objective. Scale Bar: 100 µm
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817705 25 µg £73
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817706 100 µg £129
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Description

Inositol 1,4,5-triphosphate (IP3) is a calcium-regulating second messenger that receptor involved in ER stress-induced apoptosis. IP3 receptor, type 1 (IP3R1) is a receptor for IP3 expressed on the ER which opens a calcium channel on the ER after IP3 bidning. IP3R1 is expressed in developing and mature neurons and its dysregulation may be involved in progression of certain neurodegenerative diseases. IP3R1 is also involved in autophagy, cellular energenetics, macrophage inflammasomes, and other cellular processes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 2680-2749 of rat IP3 receptor type 1.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is less reactive in human samples than mouse and rat sample in WB.

Application References

(PubMed link indicates BioLegend citation)
  1. Zhao G, et al. 2010. J. Gen. Physiol. 136(3):283-91 (WB)
RRID
AB_2801148 (BioLegend Cat. No. 817705)
AB_2801148 (BioLegend Cat. No. 817706)

Antigen Details

Structure
IP3R1 is a 2758 amino acid protein with an apparent molecular mass of 314 kD.
Distribution

Cellular distribution: Plasma membrane, endoplasmic reticulum, and nucleus.

Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Postsynaptic proteins
Antigen References

Tang T-S, et al. 2009. J. Neurosci. 29 (5) 1257-66.

Gene ID
25262 View all products for this Gene ID
UniProt
View information about IP3R1 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 2    Revision Date: 06/14/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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